Shrushti Shah
Rockford,USA
Summary
Passionate Scientist seeking opportunities in the field of Biomedical Sciences.
Overview
5
5
years of professional experience
Work History
Teaching Assistant
University Of Illinois College Of Medicine
01.2024 - Current
- Headed team of three TAs in managing Flow Cytometry course which included teaching operation of FACSymphony A1 analyzer to 18 students.
- Helped students transplant immune-compromised C57BL/6 Rag -/- mice by adoptive transfer of lymphocytes from C57BL/6 FK immunocompetent mice.
- Offered constructive feedback on student's weekly laboratory reports , helping them refine their writing skills and data representation.
Instrument Specialist
University Of Illinois College Of Medicine
08.2023 - Current
- In charge of the core flow cytometry facility at University of Illinois, College of Medicine at Rockford.
- Maintenance and operation of two flow cytometry machines; BD FACSymphony A1 analyzer and BD FACS Melody i.e. cell sorter.
- Provided technical support and guidance to fellow research scientists for operation of Flow Cytometry machines.
Laboratory Assistant
University Of Illinois College Of Medicine
10.2022 - 07.2023
- To maintain teaching laboratory for experiments and assist TAs with laboratory requirements.
Laboratory Assistant
Plazma Institute
06.2021 - 05.2022
- To maintain the high-school education laboratory for biological experiments and teach basic experimental skills to students.
Research Associate Internship
BioLim's Science and technology hub
05.2021 - 06.2021
- To understand day-to-day responsibilities and complete daily tasks of a Research Associate.
Trainee
Biological and Life Sciences, Ahmedabad University
12.2018 - 02.2019
- Learned to operate Scanning Electron Microscope (SEM) with energy dispersive X-ray Spectroscopy (EDS) (Model: JEOL JSM6010LA).
- This led to my selection as instructor of Electron microscopy workshop in tech-fest called CHIASMA in 2019 and workshop in charge and trainer of instructors in the same workshop in CHIASMA 2020.
Education
Master of Science - Medical Biotechnology
University of Illinois College of Medicine
Rockford, IL06.2024
Bachelor of Science - Life Sciences
Ahmedabad University
Ahmedabad, India05.2021
Skills
- Flow cytometry: Operation of BD FACSymphony A1 analyzer, and cell sorting using BD FACSMelody
- Cell culture techniques: Media preparation, cell line revival, passaging, worked with A549 lung carcinoma cell line, BPH, DU145, and Myc-Cap ie, PCa cell lines
- In vitro cell assays: cell viability assay, clonogenic assay, wound-healing assay, spheroid assay, trans-well migration assay, and flow cytometry assays like apoptosis assay, cell cycle analysis, and Reactive Oxygen Species (ROS) assay
- In vivo techniques: Subcutaneous and IP injections, tail snipping, and spleen extraction Worked with FVB/NJ immunocompetent mice which included subcutaneous introduction of tumor followed by drug treatments via IP and tumor-immunological analysis using flow cytometry
- Microscopy/Imaging: Compound light microscope, confocal fluorescent microscope (Olympus and Keyance), and scanning electron microscope (model: JEOL JSM6010LA)
- MS Office: Word, PowerPoint, and Excel
- Software Skills: BD FACS Diva, BD FACS Chorus, Flow Jo, GraphPad Prism, and Minitab statistical software
Research Experience
Master's Thesis Project: Investigating the Anti-cancer Effects and Underlying Cellular Mechanisms of Synergistic Drug Combination NCX 4040 and Napabucasin on Human and Murine Prostate Cancer. (Aug 2023- June 2024)
Successfully conducted in vitro research on two human PCa cell lines; BPH-cd, DU145, and one murine PCa cell line Myc-Cap followed by in vivo research on FVB/NJ immunocompetent mice to establish the combination therapy of two drugs NCX 4040 and Napabucasin. The project has significant implications for the development of a novel chemo-immunotherapeutic strategy of combination drug treatment for advanced prostate cancer.
Academic research project of Flow cytometry: (Jan- Apr 2022)
Performed the adoptive transfer of splenocytes from immunocompetent BALB/c FoxP3 mice to immunocompromised C57BL/6 Rag-/- mice and analyzed the reconstitution of T cells and B cells by weekly flow cytometry analysis.
Undergraduate Research Project: Impact of phthalate microplastics (DEP, DBP, and DEHP) on early zebrafish development. (Jan- Apr 2020)
Successfully conducted toxicity screening of different microplastics; Diethyl phthalate (DEP), Dibutyl phthalate (DBP), and Diethyl-hexyl phthalate (DEHP) on zebrafish (Danio rerio) embryos by observing the malformation at early development stages. This project contributed to understanding the environmental toxicity caused by microplastics.
Publications
Shah, S., & Kumar, A. (2022). Bioavailability, Bioconcentration, and Biomagnification of Pollutants. In Environmental Toxicology and Ecosystem (pp. 179-214). CRC Press.
Volunteering
Elected as a Treasurer of the Medical Biotechnology (MBT) Student and Association Council in 2023.
Member of Social Services Forum at Ahmedabad University, Ahmedabad, India, from 2018 to 2020.
Timeline
Teaching Assistant
University Of Illinois College Of Medicine
01.2024 - Current
Instrument Specialist
University Of Illinois College Of Medicine
08.2023 - Current
Laboratory Assistant
University Of Illinois College Of Medicine
10.2022 - 07.2023
Laboratory Assistant
Plazma Institute
06.2021 - 05.2022
Research Associate Internship
BioLim's Science and technology hub
05.2021 - 06.2021
Trainee
Biological and Life Sciences, Ahmedabad University
12.2018 - 02.2019
Master of Science - Medical Biotechnology
University of Illinois College of Medicine
Bachelor of Science - Life Sciences
Ahmedabad University
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