Shruti Sharma
Overland Park
Summary
Flow Cytometry Scientist specializing in assay development and immune profiling. Expertise in high-parameter panel design, project management, and instrument maintenance. Experienced in driving data quality and consistency through method optimization and training, contributing to successful translational research initiatives.
Overview
10
10
years of professional experience
Work History
Volunteer
Sarah Cannon Cancer Center
03.2026 - Current
- Overland Park Regional Medical Center/HCA Healthcare
- Assisted patients with transportation to treatment appointments.
- Organized and maintained volunteer schedules and assignments.
- Provided emotional support to patients and families during treatment.
Flow Cytometry Core Manager
Saliogen Therapeutics
09.2024 - 10.2024
- Led end-to-end cytometry operations, including experimental design, assay execution, and data delivery across multiple programs.
- Coordinated sample intake, assay scheduling, and stakeholder communication to deliver timely, high-quality results.
- Developed and optimized high-parameter flow panels for immune profiling and potency assays, enhancing assay precision.
- Oversaw instrument QC, maintenance, and troubleshooting (Cytek Aurora, BD Aria, S6 sorter).
- Trained scientists on cytometry workflows, increasing data consistency and success rates in experiments.
- (Company shutdown)
Principal Research Associate
Sanofi
04.2021 - 08.2023
- Led development and qualification of ligand-binding and cell-based potency assays (ELISA, MSD, flow cytometry) supporting translational and regulatory programs.
- Designed and executed immune cell–based assays (TDCC, cytokine release) using primary human T cells.
- Managed multiple concurrent projects, aligning stakeholders, timelines, and deliverables in a matrixed environment.
- Drove assay optimization, troubleshooting, and method transfer under GxP conditions.
- Authored SOPs, validation reports, and regulatory documentation.
Senior Research Associate
Biocytogen Boston Corp
07.2018 - 04.2021
- Designed and executed 18–22 color flow cytometry panels for immune profiling and mechanistic studies.
- Performed ADCC, cytotoxicity, receptor occupancy, ELISA, and MSD assays.
- Supported in vivo studies with tissue processing, cell isolation, and FACS sorting.
- Contributed to assay transfer and cross-functional collaboration across discovery programs.
Research Technician
Dana-Farber Cancer Institute
10.2016 - 04.2018
- Conducted CRISPR/siRNA gene editing and functional assays in 3D cancer models.
- Performed flow cytometry, qPCR, and Western blot for mechanistic studies.
Education
M.S. - Chemical Engineering
IIT Bombay
M.S. - Biotechnology
Kurukshetra University
B.S. - Biotechnology
Kurukshetra University
Skills
- Flow Cytometry (Spectral & Conventional)
- FACS Sorting
- High-Parameter Panel Design (18–22 colors)
- Immune Profiling (PBMCs, TILs, primary cells)
- Cell-Based Assays (ADCC, TDCC, Cytokine Release)
- Assay Development
- Optimization & Troubleshooting
- Data Analysis (Flow Jo, OMIQ, Cyto bank, Prism)
- Project Management
- Cross-Functional Collaboration
- GxP/GLP/GMP Compliance
- SOPs
- Method Validation
Publications
- Sharma S. Cancer Cell, 2020
- Sharma S. Cancer Research, 2020
- Sharma S. AACR, 2020
- Contributions to PLOS Biology, Genesis
Timeline
Volunteer
Sarah Cannon Cancer Center
03.2026 - Current
Flow Cytometry Core Manager
Saliogen Therapeutics
09.2024 - 10.2024
Principal Research Associate
Sanofi
04.2021 - 08.2023
Senior Research Associate
Biocytogen Boston Corp
07.2018 - 04.2021
Research Technician
Dana-Farber Cancer Institute
10.2016 - 04.2018
M.S. - Chemical Engineering
IIT Bombay
M.S. - Biotechnology
Kurukshetra University
B.S. - Biotechnology
Kurukshetra University