Summary
Overview
Work History
Education
Skills
Publications
Timeline
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Sreeja Edara

Lansdale,USA

Summary

Scientist I with expertise in continuous column-free chromatography and purification of AAV, mRNA, Lentivirus, and mAbs. Skilled in integrating process and analytical tools (Kascade, SEC-MALS, HPLC) for real-time monitoring. Proficient in SEC, IEX, Affinity Chromatography and analytical methods including BLI, ELISA, qPCR, and mass photometry. MIT-certified in Downstream Processing with a focus on method development and process optimization.

Overview

6
6
years of professional experience

Work History

Scientist I

ChromaTan Inc
Ambler, PA
06.2025 - Current
  • Lead analytical and process development for purification of complex biologics (AAV, mRNA, Lentivirus, mAbs).
  • Integrated Kascade continuous chromatography with SEC-MALS and Shimadzu HPLC for real-time process monitoring.
  • Designed scalable purification protocols using SEC, IEX, and Affinity Chromatography.
  • Developed analytical methods across mass photometry, ELISA, qPCR, dPCR, HPLC, and BLI to ensure product consistency.

Senior Associate Scientist II

ChromaTan Inc
Ambler, PA
11.2024 - 06.2025
  • Optimized purification workflows and enhanced analytical methods (SEC-MALS, mass photometry, ELISA, qPCR).
  • Developed mRNA quantification using Oligo-dT HPLC, enabling high sensitivity and throughput.
  • Established Lentivirus titering using custom BLI probes, improving process monitoring accuracy.

Senior Associate Scientist I

ChromaTan Inc
Ambler, PA
01.2023 - 10.2024
  • Led purification of AAV, mRNA, Lentivirus, and mAbs using continuous column-free chromatography.
  • Applied SEC, IEX, and Affinity Chromatography for process optimization.
  • Conducted binding, wash, and elution studies to improve yield and efficiency.
  • Developed purification protocols using AKTA Pure and CaptureSelect Resins.

Graduate Research Assistant

Albany College Of Pharmacy
Albany, NY
01.2021 - 12.2022
  • Expressed and purified Cytochrome P450*14 variants using mutagenesis, affinity, and ion exchange chromatography.
  • Solved 3D structure of P450*14–Losartan complex via X-ray crystallography.
  • Performed vapor diffusion crystallization, PyMOL structural analysis, and enzymatic assays.
  • Developed improved protocols for protein expression and purification.

Clinical Research Intern

Malla Reddy Hospital & St. Theresa Hospital
Hyderabad, India
08.2019 - 07.2020
  • Educated 50+ patients daily on medication use, side effects, and interactions.
  • Counseled on new prescriptions, OTC products, and Type 2 diabetes care.
  • Performed BP and glucose testing; collected medical histories for safe medication use.
  • Ensured accurate and timely prescription processing in collaboration with healthcare providers.

Education

Professional Certification Course - Downstream Processing

Massachusetts Institute of Technology
Boston, MA
07.2023

Master's - Pharmaceutical Sciences

Albany College of Pharmacy And Health Sciences
Albany, NY
12-2022

Doctor of Pharmacy/Pharm-D -

Osmania University
Hyderabad, India
08.2020

Skills

  • HPLC, Mass Photometry, ELISA
  • Western Blot, SDS PAGE, Digital/Quantitative PCR
  • Vapor Diffusion, X-Ray Crystallography, COOT, PyMOL, Enzymatic Assays
  • SEC-MALS, HPLC-FLD, Dynamic Light Scattering (DLS)
  • Chromatography (Size Exclusion, Affinity, Ion Exchange)
  • Purification of different serotypes of AAV and Lenti Virus
  • Biolayer Interferometry Technology, Nanodrop
  • Continuous process development via bench-top experiments
  • Purification of mABs and mRNA

Publications

●Structural and Biophysical Analysis of Cytochrome P450 2C9*14 and *27 Variants in Complex with Losartan (05/31/2024) - Journal of Inorganic Biochemistry

● A Prospective Observational Study on Drug Utilization Patterns and Potency of Antihypertensives and Oral Hypoglycaemics(03/30/2020) - International Journal of Pharmacy and Pharmaceutical Sciences

Timeline

Scientist I

ChromaTan Inc
06.2025 - Current

Senior Associate Scientist II

ChromaTan Inc
11.2024 - 06.2025

Senior Associate Scientist I

ChromaTan Inc
01.2023 - 10.2024

Graduate Research Assistant

Albany College Of Pharmacy
01.2021 - 12.2022

Clinical Research Intern

Malla Reddy Hospital & St. Theresa Hospital
08.2019 - 07.2020

Professional Certification Course - Downstream Processing

Massachusetts Institute of Technology

Master's - Pharmaceutical Sciences

Albany College of Pharmacy And Health Sciences

Doctor of Pharmacy/Pharm-D -

Osmania University

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Sreeja Edara