Steven E. Schultze
Louisville,KY
Summary
Professional technician with extensive hands-on experience in troubleshooting and improving complex systems. Strong focus on team collaboration and process improvement. Reliable with adaptable skill set in diagnostics, research and quality control. Known for effective problem-solving and integrating technology into existing processes.
Overview
15
15
years of professional experience
Work History
Genetic Sequencing Technician
University of Louisville School of Medicine
10.2022 - Current
- Integrated targeted genetic sequencing data with quality control data to improve and optimize processes
- Collected and analyzed quality control data for ongoing troubleshooting and process monitoring
- Analyzed genetic sequence data in highly complex regions of the genome
- Integrated label printers and other new technologies to improve lab efficiency
- Performed unsupervised cluster analysis on genetic sequencing data
- Completed master of science while working full time
- Independently organized and planned overlapping multi-step scientific protocols
- Organized and processed up to 400 samples at a time
Research Animal and Laboratory Technician
University of Louisville School of Medicine
07.2021 - 10.2022
- Independently managed 13 genetically distinct colonies of transgenic research mice
- Routinely isolated microbial DNA from fecal samples for NanoPore DNA/RNA sequencing
- Performed genotyping PCR amplification reactions for multiple strains of gene knockout mice
- Purified and prepared samples for 16S ribosomal RNA sequencing analysis of microbiome samples
- Conducted daily lab duties in BioSafety Level (BSL) 2 environments and Animal Biosafety Level(ABSL)2
- Provided succinct and organized updates regarding status of multiple research animal colonies
- Regularly attended seminars and guest speaker lectures to further education and knowledge
- Maintained inventory and oversaw ordering of supplies for daily lab operational needs
Computer Repair Technician
Ivy Technology
04.2021 - 07.2021
- Diagnosed laptop PC hardware failures
- Documented and communicated required repairs
- Performed parts replacement and necessary repairs on laptop PCs
- Worked on a team to produce high volume output of diagnostics and device repairs
- Coached others though the diagnostic process
- Exercised best judgment for determining if needed repairs were covered by warranty standards
- Communicated with management on potential improvements in operational workflow
Senior Pharmacy Analyst
Baptist Health
08.2020 - 02.2021
- Lead project to implement new features and perform upgrades to operational software
- Performed daily maintenance of Pharmacy operations software
- Instructed users to more effectively use complex computer systems
- Diagnosed and improved complex computer software and hospital logistics
- Designed and executed project plans for upgrades and changes to the IT systems
- Built and updated medication alternatives and automated alerts to improve quality of service
- Communicate with users to effectively solve issues and make appropriate updates to system functionality
Lift Operator
Park City Mountain Resort
01.2020 - 03.2020
Technical Analyst
The Cleveland Clinic
06.2019 - 12.2019
- Implemented enterprise wide software upgrades and changes hardware deployment procedures
- Identified gaps in enterprise disaster recovery plans
- Organized a team of people to perform hardware installs and validation test scripts
- Deployed improvements to disaster recovery and business continuity access
- Reviewed Cleveland's instance specific code customizations for opportunities to return to supported standard development
- Co-Lead discovery on device integration needs for Epic Beaker install
- Communicated technical requirements with clinic/hospital management
- Identified and mitigated risks and roadblocks for project completion
- Organized and managed 3rd party contractors to ensure on time delivery of contracted services
- Monitored scope of project to prevent scope creep and on-time delivery of expected upgrades
Home Remodeling
Schultze Properties
01.2012 - 06.2019
- Managed repair and install projects for complete interior and exterior home renovations
- Installation of home utilities in including electrical and plumbing repair or replacement
- Analyzed, planned, and executed repair projects from conception to completion
Swim Instructor/Lifeguard
YMCA of Dane County
01.2018 - 05.2018
Pharmacy Systems Customizations Developer/ Account Representative
Epic
01.2016 - 10.2017
- Developed customer requested fixes to pharmacy operations database
- Resolved technical issues with EMR database software and prepared related technical documentation
- Effectively communicated technical information to hospital leadership and clinicians on a daily basis
- Wrote and executed custom database queries in M and kbSQL
- Demoed software use cases and new features to managers, users, and IT administrators
- Provided project/account management support to three unique Pharmacy implementations
- Adapted to changing demands, learned pharmacy operations and hospital logistics, and balanced multiple projects simultaneously to help customers maintain and improve EMR implementation
- Oversaw and supported customer management of server redundancy and production downtime backup data access
Undergraduate Research Lab Assistant
Stauffacher Group/Purdue University Biology
05.2014 - 08.2015
- Analyzed research articles to recreate experiment instructions
- Documented and refined step by step instructions for repeatable protein production experiments
- Prepared Isolated Protein samples for crystallization and biophysical analysis
- Designed and executed independent research project on fluorescent ATP analog binding kinetics
- Purified protein via Ni resin Chromatography in automated and manual columns
- Transformed and expressed IPTG inducible plasmids in D5α and BL21 E
- Coli cells
- Independently designed a ligation experiment to attach Green Fluorescent Protein
- Programmed robotic pumps for extended HPLC (High Pressure Liquid Chromatography) experiments
WSI Swim Instructor
Purdue Recreational Sports
01.2014 - 08.2015
Laboratory Intern
Eurofins MWG Operon
05.2013 - 08.2013
- Sequenced DNA using high throughput fluorescence labeled Sanger sequencing techniques
- Followed documented instructions to ensure correct process was used on all samples
- Organized and cataloged stored samples and DNA primers
- Performed dilution of customer provided samples to ensure proper results
- Performed PCR preparations on unfiltered and gel samples from customer
- Operated laboratory equipment including thermo-cyclers, liquid handling robots, Nanodrop spectrophotometer and ABI 3730xI DNA Analyzer
LifeGuard
YMCA/WaveTek
04.2010 - 08.2012
Education
Masters Degree - Biochemistry & Molecular Genetics
University of Louisville School of Medicine
Louisville, KY12.2024
Bachelor of Science - Biology, Chemistry(minor), Computer Science(minor)
Purdue University, School of Science
West Lafayette, IN05.2015
Skills
- R, Python, SciKitLearn, Java, C
- MIPS, X86, HTML, M(Mumps)
- Outlook, Word, Excel
- Research Animal Handling, Care, and Euthanasia
- PCR
- NGS sample preparation
- Protein Production & Isolation
- Plumbing
- Troubleshooting, and Quality Control
Timeline
Genetic Sequencing Technician
University of Louisville School of Medicine
10.2022 - Current
Research Animal and Laboratory Technician
University of Louisville School of Medicine
07.2021 - 10.2022
Computer Repair Technician
Ivy Technology
04.2021 - 07.2021
Senior Pharmacy Analyst
Baptist Health
08.2020 - 02.2021
Lift Operator
Park City Mountain Resort
01.2020 - 03.2020
Technical Analyst
The Cleveland Clinic
06.2019 - 12.2019
Swim Instructor/Lifeguard
YMCA of Dane County
01.2018 - 05.2018
Pharmacy Systems Customizations Developer/ Account Representative
Epic
01.2016 - 10.2017
Undergraduate Research Lab Assistant
Stauffacher Group/Purdue University Biology
05.2014 - 08.2015
WSI Swim Instructor
Purdue Recreational Sports
01.2014 - 08.2015
Laboratory Intern
Eurofins MWG Operon
05.2013 - 08.2013
Home Remodeling
Schultze Properties
01.2012 - 06.2019
LifeGuard
YMCA/WaveTek
04.2010 - 08.2012
Masters Degree - Biochemistry & Molecular Genetics
University of Louisville School of Medicine
Bachelor of Science - Biology, Chemistry(minor), Computer Science(minor)
Purdue University, School of Science
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