Steven E. Schultze

• Analyzed research articles to recreate experiment instructions
• Documented and refined step by step instructions for repeatable protein production experiments
• Prepared Isolated Protein samples for crystallization and biophysical analysis
• Designed and executed independent research project on fluorescent ATP analog binding kinetics
• Purified protein via Ni resin Chromatography in automated and manual columns
• Transformed and expressed IPTG inducible plasmids in D5α and BL21 E
• Coli cells
• Independently designed a ligation experiment to attach Green Fluorescent Protein
• Programmed robotic pumps for extended HPLC (High Pressure Liquid Chromatography) experiments

● Implemented and executed workflow improvements for end-to-end testing of infectious disease samples in a CLIA and CAP certified clinical diagnostic environment utilizing multiplexed PCR technology
● Used semi-automated liquid handling robots and high-throughput lab instruments including KingFisher Flex, Accufill, VeritiPro, and QuantStudio 12K
● Instructed and onboarded other instrument users in workflow best practices in a CAP and CLIA certified environment
● Adhered to laboratory quality control policies and SOPs and monitored and instructed new hires on SOP adherence
● Reviewed all SOPs and workflow updates in a timely manner and ensured all SOP updates were implemented in real time for workflow processes
● Identified problems that may adversely affect testing performance or reporting of test results and communicated with appropriate stakeholders

• Integrated targeted genetic sequencing data with quality control data to improve and optimize processes
• Collected and analyzed quality control data for ongoing troubleshooting and process monitoring
• Analyzed genetic sequence data in highly complex regions of the genome
• Integrated label printers and other new technologies to improve lab efficiency
• Performed unsupervised cluster analysis on genetic sequencing data
• Completed master of science while working full time
• Independently organized and planned overlapping multi-step scientific protocols
• Organized and processed up to 400 samples at a time

• Independently managed 13 genetically distinct colonies of transgenic research mice
• Routinely isolated microbial DNA from fecal samples for NanoPore DNA/RNA sequencing
• Performed genotyping PCR amplification reactions for multiple strains of gene knockout mice
• Purified and prepared samples for 16S ribosomal RNA sequencing analysis of microbiome samples
• Conducted daily lab duties in BioSafety Level (BSL) 2 environments and Animal Biosafety Level(ABSL)2
• Provided succinct and organized updates regarding status of multiple research animal colonies
• Regularly attended seminars and guest speaker lectures to further education and knowledge
• Maintained inventory and oversaw ordering of supplies for daily lab operational needs

• Diagnosed laptop PC hardware failures
• Documented and communicated required repairs
• Performed parts replacement and necessary repairs on laptop PCs
• Worked on a team to produce high volume output of diagnostics and device repairs
• Coached others though the diagnostic process
• Exercised best judgment for determining if needed repairs were covered by warranty standards
• Communicated with management on potential improvements in operational workflow

• Lead project to implement new features and perform upgrades to operational software
• Performed daily maintenance of Pharmacy operations software
• Instructed users to more effectively use complex computer systems
• Diagnosed and improved complex computer software and hospital logistics
• Designed and executed project plans for upgrades and changes to the IT systems
• Built and updated medication alternatives and automated alerts to improve quality of service
• Communicate with users to effectively solve issues and make appropriate updates to system functionality

• Implemented enterprise wide software upgrades and changes hardware deployment procedures
• Identified gaps in enterprise disaster recovery plans
• Organized a team of people to perform hardware installs and validation test scripts
• Deployed improvements to disaster recovery and business continuity access
• Reviewed Cleveland's instance specific code customizations for opportunities to return to supported standard development
• Co-Lead discovery on device integration needs for Epic Beaker install
• Communicated technical requirements with clinic/hospital management
• Identified and mitigated risks and roadblocks for project completion
• Organized and managed 3rd party contractors to ensure on time delivery of contracted services
• Monitored scope of project to prevent scope creep and on-time delivery of expected upgrades

• Managed repair and install projects for complete interior and exterior home renovations
• Installation of home utilities in including electrical and plumbing repair or replacement
• Analyzed, planned, and executed repair projects from conception to completion

• Developed customer requested fixes to pharmacy operations database
• Resolved technical issues with EMR database software and prepared related technical documentation
• Effectively communicated technical information to hospital leadership and clinicians on a daily basis
• Wrote and executed custom database queries in M and kbSQL
• Demoed software use cases and new features to managers, users, and IT administrators
• Provided project/account management support to three unique Pharmacy implementations
• Adapted to changing demands, learned pharmacy operations and hospital logistics, and balanced multiple projects simultaneously to help customers maintain and improve EMR implementation
• Oversaw and supported customer management of server redundancy and production downtime backup data access

• Sequenced DNA using high throughput fluorescence labeled Sanger sequencing techniques
• Followed documented instructions to ensure correct process was used on all samples
• Organized and cataloged stored samples and DNA primers
• Performed dilution of customer provided samples to ensure proper results
• Performed PCR preparations on unfiltered and gel samples from customer
• Operated laboratory equipment including thermo-cyclers, liquid handling robots, Nanodrop spectrophotometer and ABI 3730xI DNA Analyzer