Stuart H. Cole
Brighton
Summary
Scientist with extensive biotechnology experience, specializing in in vivo pharmacology and molecular biology. Proven track record in managing complex studies and mentoring teams, with expertise in in vivo experiments and ex vivo processing. Focus on innovative solutions in genetic and regenerative medicine, contributing significantly to pre-clinical research and fostering effective cross-functional collaboration.
Overview
10
10
years of professional experience
Work History
Scientist I
Aera Therapeutics
Boston
06.2023 - Current
- Leading efforts in biodistribution/tropism in genetic medicine platforms
- Coordinating in vivo studies across different cross-functional groups
- Leading studies with bench-top molecular ex-vivo workflows: processing tissues for flow cytometry and qPCR
- Strategizing on capturing PD effect with timing and tools: qPCR, ELISA, histology, flow cytometry, and IVIS
- Teaching/mentoring scientists
- Setting up new vivarium: conducted needs assessment for supplies, tables, engineering controls, colony management software, SOPs on study execution: dosing and tissue/blood collection
- IACUC protocol amendment submissions
- Managing in-vivo team: study design, coordinating across departments from DMPK and formulation groups for implementation of studies
Tecnico Superior 4: In vivo pharmacology team lead
Faes Farma
Leioa
09.2021 - 05.2023
- Managing and advising all in vivo pharmacology needs
- Scheduling experiments
- Instructing staff on in vivo techniques as needed
- Working on multiple projects in tandem: model development and performing experiments
- Providing needs assessments for all laboratory needs: vivarium remodel, equipment, etc
- Helping with molecular biology workflow if needed
- Consulting with CROs when project needs exceed the pharmacology team’s ability
- Hiring board for IDi (R&D) biology department
Senior Scientist I/Associate Scientist II
Novartis Institute of BioMedical Research (NIBR San Diego)
San Diego
03.2020 - 08.2021
- Company Overview: Genomics Institute of the Novartis Research Foundation (GNF)
- Managed and conducted in-vivo experiments for PK/PD screenings for exploratory efforts and efficacy studies in disease areas of peripheral arterial disease and chronic kidney disease
- Training scientists: in-vivo techniques and equipment (Digigait, Laser Doppler Radar, Treadmill)
- Authored and managed amendments for IACUC approval
- Advised on the hiring board for potential post-doctoral candidates and scientists
- Assisted pre-clinical projects through safety studies
- Established different animal models in connective tissue injury, renal, and cardiovascular pathology
- Passaged Embryonic Stem Cells, HK2, and HEK cells to assist in-vitro biologists
- Awards & Prestigious Projects
- 3R’s award for “Refinement” (2017)
- Collaborated with NASA and others in “In-vivo data and tissue collection for Rodent Research-6 (RR-6).” Exploring muscle loss/maintenance in space, and efficacy of implanted pump in microgravity
Intern
Novartis Institute of BioMedical Research (NIBR San Diego)
San Diego
11.2014 - 01.2015
- Company Overview: Genomics Institute of the Novartis Research Foundation (GNF)
- Supported the histology department by performing full necropsies on mice and rats for control samples.
- Embedding tissue (paraffin and frozen). Sectioning for mounting.
- Uploaded scanned slides into digital media.
- Assisted with track project in dosing, preparing rodents for surgery, and running PD studies.
- Genomics Institute of the Novartis Research Foundation (GNF)
Education
Bachelor of Science - Exercise Science
Southern Oregon University
06-2011
Skills
In vivo
- Survival surgeries
- Rodent models
- Murine Kidney disease models
- Functional Readouts
- Murine Hindlimb Ischemia
- Murine Bleomycin Induced skin-fibrosis
- Urticaria models
- Eosinophilic Esophagitis
- Rat Spinal Disc injury models
- Rat Achilles tendon injury
- In-vivo functional readouts/equipment operator
- Rodent gait analysis
- Rodent exercise treadmill
- Laser Doppler radar
- Live imaging
- Dosing
- Blood retrieval
- Bone marrow extraction
- Mouse handling
- Rat handling
- Hamster handling
- Full body perfusion
In Vitro, Ex Vivo, and Molecular Biology
- Flow cytometry (multi-color)
- Mammalian cell culture
- Lentivirus production
- Automated lab equipment
- Histology: parafinization, embedding, microtome
- RNA isolation for qPCR
- QC RNA: Stunner plate, Tapestation
- Protein assays: ELISA, MSD, Luminex
- Luminescence assays: One-Glo, Nano-Glo
Publications
Pharmacological inhibition of porcupine induces regression of experimental skin fibrosis by targeting Wnt signaling., Chen CW, Beyer C, Liu J, et al., Ann Rheum Dis., 2017, 76, 4, 773-778, 10.1136/annrheumdis-2016-210294
In Vivo Skills
- Survival surgeries, administered anesthesia, analgesics, and assessed animals for pain,
- Rodent models implemented: hCD34+ mice (NSG and NSG), Nalm6 in humanized mice (PBMC engrafted),
- Murine Kidney disease models: UUO, Ischemia Reperfusion, novel renal model, anti-Glomerular Basement Membrane induced nephritis, Functional Readouts: clinical urine and blood markers, tGFR (transdermal glomerular filtration rate), and Inulin GFR.,
- Fibrosis models: murine hindlimb ischemia: a peripheral arterial disease model, murine bleomycin induced skin-fibrosis (Scleroderma mouse model), Rat Spinal Disc injury models: Lumbar Injury: puncture and enzymatic; tail disc injury, and lateral tail compression, Rat Achilles tendon injury: partial tenotomy model
- Immune stimulating disease models: urticaria models: c48/80, Passive anaphylaxis model, Eosinophilic Esophagitis: Peanut and dust mite models, ,
- In-vivo functional readouts/equipment operator, Rodent gait analysis (Digigait), Rodent exercise treadmill (PanLab), Laser Doppler radar: functional readout for blood flow (flux%), Live imaging: fluorescence and x-ray/CT scan
- Basic in vivo skills: Dosing: intravenous (tail IV and RO), subcutaneous (SQ), intraperitoneal (IP), oral gavage (PO), intradermal (ID), Blood retrieval: cardiac puncture, tail (distal/lateral tail vein, tail snip), submandibular, saphenous vein, retro-orbital, vena cava, Bone marrow extraction (syringe plunger method and centrifugation) mouse, rat, and hamster handling, full body perfusion via cardiac
Affiliations
- California Junior College All-American wrestler
- Brazilian Jiu-Jitsu Black belt
- Play bass guitar, guitar, mandolin, and ukulele
In vitro, ex vivo, and molecular skills
- Mammalian cell culture & sterile technique, Lentivirus production, Operated automated lab equipment: Labcyte Echo, HTRF, and MSD, Histology: PPFE processing and tissue embedding,
- Molecular skills: RNA isolation for qPCR: column-based methods and automated (Kingfisher), ELISA and MSD, Flow Cytometry: splenocyte isolation wand running assay for immune cell profiling and measuring PD effect (Attune and Novocyte)
Timeline
Scientist I
Aera Therapeutics
06.2023 - Current
Tecnico Superior 4: In vivo pharmacology team lead
Faes Farma
09.2021 - 05.2023
Senior Scientist I/Associate Scientist II
Novartis Institute of BioMedical Research (NIBR San Diego)
03.2020 - 08.2021
Intern
Novartis Institute of BioMedical Research (NIBR San Diego)
11.2014 - 01.2015
Bachelor of Science - Exercise Science
Southern Oregon University
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