SANJAY MISHRA
RESEARCH SCIENTIST
Columbus,OH
Summary
As a skilled cellular and molecular biologist with 13 years of experience in Cancer Biology research, I have successfully managed my lab at the Ohio State University, mentored Post-doc & graduate students, and demonstrated strong leadership skills as a scientific leader. I have achieved success in securing federal grants, including DoD and NIH grants, with exceptional grant-writing skills that scored well. I have also published and reviewed several articles and progress reports, presented scientific data at 20+ national and international conferences, and designed and executed innovative research projects using creative approaches and techniques to achieve research outcomes. My excellent written and verbal communication skills have enabled me to effectively present data and collaborate with fellow researchers.
Overview
13
13
years of professional experience
Work History
Research Scientist
The Ohio State Wexner Medical Center
Columbus, OH
06.2022 - Current
- Led the cross-collaborative research studies and mentored the researchers focused on identifying the role of Slit2 and S100A7 in breast tumorigenesis through modulating tumor microenvironment.
- Establish the novel role of Slit2 protein in breast tumor growth and metastasis by modulating the recruitment and plasticity of tumor-associated macrophages. The procedure involved: MMTV-PyMT and Xenograft mouse models, metabolomics, flow cytometry, cell-based assays, and ELISA. Achievements: These studies were published in Cancer Res. 2021, Front Immunol. 2021 and Mol Onc. 2022.
- Evaluating the therapeutic effects of different inhibitors or therapeutic compounds such as FPS-ZM1, Azeliragon, Reboxetine, ASB-14780, and Tasquinimod in inhibiting the breast and lung tumorigenesis using cell-based assays, biochemical assays, in-vitro and in-vivo mouse models. Achievements: Wrote manuscript as a co-corresponding author.
- Wrote and submitted revised federal and non-federal grants as a Co-I which scored well in the last submissions. Achievements: Awarded bridge funding, OSU for analyzing the role of S100A7 in obesity-associated TNBC.
- Wrote, compiled and edited assigned portions of proposals and project reports to achieve grant writing and publication objectives.
- Managed laboratory personnel and monitored team performance and work quality.
Postdoctoral Research Fellow
Ohio State University Wexner Medical Center
Columbus, OH
05.2019 - 06.2022
- Led the collaborative studies and mentored the researchers focused on identifying the novel role of S100A7 in regulating obesity-associated TNBC (Major Achievement: Filed US Patent for this study: 63/429,664; 2022).
- Generated a novel S100A7 whole-body knock-out transgenic mouse model and performed a series of in vivo experiments to test the hypothesis that whole-body deletion of S100A7 protects from high-fat diet-induced obesity. Procedures involved analysis of food intake study, body weight change, echoMRI, insulin/glucose tolerance test, CLAMS assay, RNA-seq, 16S NGS, Adipokine array, ELISA, and histopathology.
- Determined the therapeutic effects of our developed novel human S100A7 neutralizing antibody against TNBC using multiple different approaches: 3D-Organoid culture, Orthotopic Xenograft mouse model, migration/invasion assay.
- Mentored a graduate student during her research project, which uncovered a functional role of miR-1 in cisplatin resistivity associated with aggressiveness of non-small cell lung cancer. The experimental design included: In vitro combination analysis, integrated OMICs approach, in vivo models, and In-situ hybridization.
- Identify the Serum Amyloid A2 (SAA2) as a potential liquid biopsy-based protein biomarker for early detection and prognosis of African American TNBC using the plasma proteomics approach. Led to the nomination by The Ohio State University for submission of a full-length application for funding at the V Foundation Cancer Research.
- Developed and filed patent invention disclosure for generating S100A7 neutralizing antibodies and S100A7 whole body knock-out mice model for treatment of obesity-associated metabolic diseases, especially breast cancer.
- Identify the functional role of S100A9 in enhancing the small cell lung cancer growth using RNA-seq, spheroid culture, drug screening, Western Blot, S100A9 whole body knock out, and SCLC PDX mouse models.
Pelotonia Post Doctoral Fellow
Ohio State University Wexner Medical Center
Columbus, OH
04.2016 - 04.2019
- Initiated a completely new project in Ganju’s lab to study the functional role of S100A7 in regulating invasive breast cancer growth and metastasis through generating an immunosuppressive tumor microenvironment (TME).
- Established humanized patient-derived xenograft (PDX) mouse model of breast cancer and evaluated the anti-cancer effect of anti-inflammatory drugs using various cell-based assays including flow cytometry and immunocytochemistry.
- Decipher the breast TME using cutting-edge tools such as Co-Detection by Indexing (CODEX) and CRISPR-Cas9.
- Developed MMTV-rtTA;TetO-mS100a7a15-C3-TAG triple-negative breast cancer (TNBC) mouse model and evaluated the impact of S100A7 overexpression on TNBC growth and metastasis. Procedures involved transgenic mice colony maintenance, tumor harvesting, and immune cell profiling using flow cytometry and CODEX.
- Uncovered preliminary evidence for a novel role of S100A7 in breast tumor microbiota. Served as the basis for one institutional (PIIO) funded grant. Achievements: Published a partial part of this study as a co-corresponding author in Molecular Oncology journal.
- Mentored a graduate student, including guidance on literature search, brainstorming hypotheses, editing the thesis report, and publishing this study in the Molecular Oncology journal. Thesis titled: “Tumor Commensal Microbiota Activates an S100A7-TLR4-STAT-3 Signaling to Induce Chronic Inflammation and Consequent Growth and Metastasis of Breast Cancer.”
- Designed and performed a series of in vitro and in vivo experiments to test the hypothesis that cPLA2 blockade attenuates S100A7-mediated breast tumorigenicity by inhibiting the immunosuppressive TME. Achievements: Mishra et al, J Exp Clin Cancer Res, 2022 (share first and co-corresponding authorship).
- Maintained field equipment and performed troubleshooting techniques to keep tools fully operational.
Graduate Researcher
Council Of Scientific And Industrial Research
Lucknow, UP, India
07.2010 - 04.2016
- Initiated a new project to decipher the role of miRNAs in breast cancer. A cross-collaborative study with the King George's Medical University, Lucknow, and CSIR- Institute of Genomics and Integrative Biology, New Delhi, India.
- Identified the potential diagnostic as well as prognostic significance of circulating miRNAs in breast cancer through miRNA profiling of breast cancer patient samples using TaqMan MicroRNA Assays. Led to a publication in Cancer Lett. 2015.
- Investigated the therapeutic effects of different phytochemicals on cancer chemoprevention using different in-vitro and in-vivo systems; Combination index and synergy quantification for drug treatment were calculated using the Chou-Talalay method (Compusyn software).
- Collaborated with other lab members which led to the development of novel protein-based biomarkers and auto-antibodies for breast cancer prognosis. Led to a publication in J Proteomics. 2016 and Proteomics Clin Appl. 2016.
- Analyze the genotoxicity of different commercial compounds using different in vitro and in vivo toxicity assays such as micronuclei assay, chromosomal aberration analysis, and DNA damage response.
- Collaborated with other researchers to evaluate the therapeutic potential of nano drug delivery system using ascitic and chemically induced breast cancer mouse models. This led to a publication in many peers reviewed journals.
Education
Ph.D. - Biological Science
CSIR-Indian Institute of Toxicology Research
Lucknow, India04.2016
Master of Science - Zoology
University of Calcutta
Kolkata, India09.2009
Bachelor of Science - Zoology
University of Calcutta
Kolkata, India07.2007
Skills
- BIOCHEMISTRY: SDS-PAGE, Western Blot, Protein array analysis, ELISA, Lyophilization, 2D-PAGE, protein enrichment, Protein Quantification, Enzyme assays (Fluorescence, Colorimetric and Bioluminescence), Immunoprecipitation, Flow Cytometry, Fluorescence microscopy, CODEX microscopy, ICC, Immunohistochemistry, Senescence (eg β gal assay) and Apoptotic assays
- MOLECULAR BIOLOGY: Transformation, transfection, PCR, q-PCR, RT-PCR, miRNA extraction and amplification, miRNA profiling, Nucleic acid (DNA/RNA) extraction for RNA-Seq or 16S NGS study, Nucleic acid quantification, Agarose gel electrophoresis, Site directed mutagenesis, Colony picking & screening, Cloning, Gel extraction, Plasmid Preparation, mouse genotyping and developing PCR strategy for maintaining transgenic animals
- CELL CULTURE: Animal and human cell lines culture, 3D-organoid or spheroid culture, primary cells culture, toxicity assays such as micronuclei and chromosomal aberration assays, cytotoxicity assay, drugs screening, combination index analysis, transwell migration or invasion assays, proliferating assays, E coli (DH5-alpha) culture, Transient transfection in different mammalian cell lines, Cell passaging, Generation of stable clones for miRNA or gene overexpression or downregulation, CRISPR Cas9 for gene editing, Drug resistant clones generation
- IN-VIVO WORK: Engraftment of PDX in humanized mice model, generation and maintenance of different syngeneic and xenograft mice models, maintenance of spontaneous cancer models such as C3(1)-Tag, MMTV-PyMT and MMTV-Erbb2-Cre transgenic strains, generation and maintenance of whole body and organ specific knock out or overexpression transgenic mice model
- SOFTWARE: Microsoft Office, Bioinformatics (BLAST, CLUSTAL W, Expasy, Oligo Analyser, EndNote, miRNA target identification, gene expression analysis using online tools (eg Oncomine, Gent2 and cBioPortal genome), Survival analysis using online tools and SPSS, Gen 5 for Synergy H4 Microplate Reader, Graph Pad Prism, Flowjo for flow cytometry data analysis, Compusyn for drug combination index analysis, LI-COR Image Studio, CODEX and IHC data analysis using Image J platform
- SOFT: Supervising different research projects, mentoring undergraduate and graduate students, writing and submitting NIH and DoD grants, Quantitative data analysis, Exceptional communication, Strong interpersonal, time management and organizational skills, Curiosity, Excellent problem-solving abilities, Professional Integrity, and collaborative work
- CERTIFICATIONS: Project Management Basics, BSL2 certification, OSU ULAR Mouse Training, and HIPAA Training
Accomplishments
- Pelotonia Postdoctoral Fellowship, The Ohio State University, Columbus, OH (May 2017)
- Research Fellowship Award, University Grant Commission, Government of India (December 2009)
Selected Publications
- Mishra S*, Charan M, Shukla RK, Agarwal P, Misri M, Verma AK, Ahirwar DK, Siddiqui J, Kaul K, Sahu N, Vyas K, Garg AA, Khan A, Miles WO, Song JW, Bhutani N, Ganju RK*. cPLA2 blockade attenuates S100A7-mediated breast tumorigenicity by inhibiting the immunosuppressive tumor microenvironment. J Exp Clin Cancer Res. (2022) In press. DOI: 10.1186/s13046-021-02221-0. (*correspondence). IF: 12.6
- Ahirwar DK, Charan M, Mishra S, Verma AK, Shilo K, Ramaswamy B, Ganju RK. Slit2 Inhibits Breast Cancer Metastasis by Activating M1-Like Phagocytic and Antifibrotic Macrophages. Cancer Res. 2021 Oct 15;81(20):5255-5267. IF: 13.3
- Wilkie T, Verma AK, Zhao H, Charan M, Ahirwar DK, Kant S, Pancholi V, Mishra S*, Ganju RK*. Lipopolysaccharide from the commensal microbiota of the breast enhances cancer growth: role of S100A7 and TLR4. Mol Oncol. 2021 May 9;. doi: 10.1002/1878-0261.12975. (*correspondence). IF: 7.4
- Charan M#, Das S#, Mishra S#, Chatterjee N, Varikuti S, Kaul K, Misri S, Ahirwar DK, Satoskar AR, Ganju RK. Macrophage migration inhibitory factor inhibition as a novel therapeutic approach against triple-negative breast cancer. Cell Death Dis. 2020 Sep 17;11(9):774. (#Equal contribution). IF: 9.6
- Mishra S, Srivastava AK, Suman S, Kumar V, Shukla Y. Circulating miRNAs revealed as surrogate molecular signatures for the early detection of breast cancer. Cancer Lett. 2015 Dec 1;369(1):67-75. IF: 9.7
- Google scholar: https://scholar.google.ca/citations?user=XYT53k8AAAAJ&hl=en
Selected Presentations
- Platform presentation at “Center for Cancer Metabolism Seminar Series” at OSU, Columbus December 2022
- Platform presentation at “Pelotonia Institute for Immuno-Oncology (PIIO)” at OSU, Columbus January 2021
Career & Professional Development
- Guest Associate Editor, Frontier in Cell and Developmental Biology January 2021-Present
- Grant Reviewer, The Ohio State Chapter of Sigma Xi January 2018-2019
- Served as Reviewers for Peer-reviewed Journals (e.g., Cancer Letters, PLOS One, Frontiers group, IJC, JECCR). January 2020-Present
- Provided Assistance as a Rider to establish a fundraising program for Cancer Research at PIIO May 2017- May 2019
- Associate Member of American Association for Cancer Research December 2018-Present
Languages
English
Full Professional
Hindi
Native/ Bilingual
https://www.linkedin.com/in/sanjay-mishra-ph-d-40a65b26
Timeline
Research Scientist
The Ohio State Wexner Medical Center
06.2022 - Current
Postdoctoral Research Fellow
Ohio State University Wexner Medical Center
05.2019 - 06.2022
Pelotonia Post Doctoral Fellow
Ohio State University Wexner Medical Center
04.2016 - 04.2019
Graduate Researcher
Council Of Scientific And Industrial Research
07.2010 - 04.2016
Ph.D. - Biological Science
CSIR-Indian Institute of Toxicology Research
Master of Science - Zoology
University of Calcutta
Bachelor of Science - Zoology
University of Calcutta
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