SUGANYA D

Position Overview: Key Responsibilities: Skills and Qualifications: Learning Outcomes:

Location: Boston, Massachusetts, United States (On-site)
Department: Renal
Duration: December 2024 – Present (4 months, Full-Time)

The Internship Trainee working in the Renal Department at Brigham and Women's Hospital, gaining hands-on experience in clinical and research settings. The role involves supporting ongoing research projects focused on renal physiology and pathology, utilizing a range of advanced molecular and cellular techniques.

• Laboratory Techniques & Analysis:
  Perform SDS-PAGE for protein separation.
  Execute Western Blotting to analyze protein expression.
  Conduct qPCR (Real-Time Polymerase Chain Reaction) assays for gene expression analysis.
  Carry out RNA Isolation procedures to prepare samples for downstream applications.
  Run ELISA assays to quantify proteins and other biomolecules.
• Cell Culture & Molecular Biology:
  Maintain Mammalian Cell Culture environments ensuring cell viability and consistency.
  Perform Transfection experiments to introduce nucleic acids into cells.
  Conduct Seeding procedures to prepare cells for various assays.
• Imaging & Data Analysis:
  Use Confocal Microscopy for high-resolution imaging of cellular structures.
  Employ ImageJ software to analyze and quantify image data.
• Technical Proficiency: Familiarity with molecular biology techniques, immunoassays, and cell culture practices.
• Attention to Detail: Ability to follow precise protocols and accurately record experimental results.
• Analytical Skills: Strong problem-solving capabilities and proficiency in data analysis.
• Team Collaboration: Excellent communication skills and a willingness to collaborate within a multidisciplinary team.
• Learning Orientation: A proactive approach to learning new techniques and applying them to renal research.
• Gain practical expertise in the application of advanced molecular biology and biochemical techniques.
• Develop a comprehensive understanding of renal physiology and the molecular mechanisms underlying kidney diseases.
• Enhance skills in experimental design, data collection, and analysis within a high-caliber research environment.
• Collaborated with team members for successful completion of research projects on time and within budget.
• Completed research, compiled data, and assisted in timely reporting.
• Conducted literature reviews to identify relevant research articles and support project objectives.
• Completed research, compiled data, updated spreadsheets, and produced timely reports.
• Maintained detailed records of experimental procedures, providing transparency and replicability of results upon request by supervisors or collaborators.
• Attended meetings and presentations to learn project details.

• Investigated molecular mechanisms of cervical cancer caused by high-risk human papillomavirus (HPV) strains to understand disease progression
• Performed network analysis using bioinformatics tools to identify critical pathways and gene clusters involved in cancer development
• Conducted differential gene expression studies to pinpoint genes with significant expression changes, focusing on potential therapeutic targets
• Proposed novel drug development targets, contributing to advancements in personalized medicine and early detection strategies

• Gained comprehensive knowledge in Quality Assurance (QA) processes, including creating and reviewing standard operating procedures (SOPs)
• Hands-on experience in Quality Control (QC) with analytical testing techniques to ensure compliance with pharmaceutical standards
• Actively contributed to Production Management, overseeing manufacturing processes and ensuring adherence to Good Manufacturing Practices (GMP)
• Developed skills in regulatory compliance by assisting with the documentation required for audits and inspections

• Conducted a network analysis to explore genetic regulators associated with Crohn's Disease, focusing on identifying novel therapeutic targets
• Investigated the complex interplay of genetic, immune, and environmental factors contributing to the disease's multifactorial etiology
• Presented findings on key hub genes and their role in Crohn's Disease progression, emphasizing their potential for therapeutic interventions