TONY LAMARK JAMES
Washington
Summary
Postdoctoral research fellow with expertise in advanced research techniques and methodologies. Proven ability to conduct independent research projects while adhering to rigorous scientific protocols. Committed to expanding knowledge and contributing to innovative research outcomes. Looking for a new opportunity that offers growth and development.
Overview
18
18
years of professional experience
Work History
Graduate Fellow/ Post-Doctoral Fellow
National Institute of Health NIH
Bethesda
01.2019 - Current
- Identification of activation of innate responses by HERV-K/HML-2 envelope via interaction with TLR-2 in a subset of amyotrophic lateral sclerosis contributes to proinflammatory immune responses.
- National Institute of Neurological Disorders and Stroke
Research Specialist II
Johns Hopkins University School of Medicine
Baltimore
01.2016 - 01.2017
- Conducted single cell analysis of HCV/HBV viral burden in patient liver biopsies using LCM single cell isolation.
- Evaluated interferon stimulated gene (ISG) expression in HIV/HCV patients on HAART treatment.
- Department of Infectious Disease
Associate Scientist
BioReliance
Rockville
01.2015 - 01.2016
- Following SOPs and GMP I conducted test of clinical and manufactured samples for microbial and fungal contamination.
- The testing was conducted in a hydrogen peroxide class 5 Isolator sterility suite camber.
- Sterility Department
Graduate Student
Drexel University College of Medicine
Philadelphia
01.2013 - 01.2015
- Evaluated the role of Vpr variants in the process of neurocognitive impairment in HIV-1 patients.
- Department of Microbiology and Immunology, Department of Molecular Medicine and Infectious Disease
Student Researcher
Arkansas State University
State University
01.2011 - 01.2012
- Using molecular modeling techniques to design efficient drug delivery systems.
- Using molecular modeling to develop novel solar panel materials.
- Department of Chemistry and Physics
Student Researcher/ Assistant
Arkansas State University
State University
01.2008 - 01.2011
- Using molecular modeling techniques to identify molecular targets of thalidomide to obtain better understanding of biological activities.
- Specialized in Conventional and Microwave Organic (CEM) synthesis (Diels-Alder, Cyclic Imide, Thalidomide Derivatives).
- Department of Chemistry and Physics
Education
PhD. - Microbiology, Immunology, and Tropical Medicine
George Washington University
Washington D.C.08-2024
Masters of Science - Microbiology and Immunology
Drexel University College of Medicine
Philadelphia, PA05-2015
Bachelors of Arts - Chemistry
Arkansas State University
State University, AR03-2011
Skills
- Cryosectioning frozen and paraffin-embedded tissues
- Immuno-cytochemistry (ICC)
- Immunohistochemistry (IHC)
- Ficoll PBMC isolation
- Sterile technique in tissue and cell culture
- Neuronal/glial differentiation from induced pluripotent stem cells (iPSCs)
- DNA/RNA isolation from human and murine neuronal tissues
- Flow cytometry
- Quantitative polymerase chain reaction (qPCR)
- Digital droplet PCR
- DNA long-read sequencing (Nanopore)
- Protein analysis by Western blot (WB)
- Antibody titer quantification by indirect ELISA
- Proficiency in Prism
- Proficiency in R
Awards Scholarships
- Graduate Fellowship, NIH NINDS- National Institute of Health, National Institute of Neurological Disorders and Stroke, 08/01/20, 05/01/24
- Intramural AIDS Research Fellowship, OAR- Office of AIDS research, 08/01/19, 05/01/20
- John G. Rango's Fellowship in Infectious Disease, Johns Hopkins University School of Medicine, 01/01/16, 12/31/16
- Undergraduate Research and Mentoring Scholarship, URM, 01/01/10, 12/31/10
- 2nd Place Chemistry and Biochemistry category, Arkansas INBRE Conference, Fayetteville, AR, 01/01/09, 12/31/09
Publications
- Antibody response to hml -2 may be protective in amyotrophic lateral sclerosis., Garcia-Montojo, M., Simula, E. R., Fathi, S., McMahan, C., Ghosal, A., Berry, J. D., Cudkowicz, M., Elkahloun, A., Johnson, K., Norato, G., Jensen, P., James, T., Sechi, L. A., Nath, A., Annals of Neurology, 92, 5, 782-792, 2022, https://doi.org/10.1002/ana.26466
- Locus coeruleus in memory formation and Alzheimer's disease., Tony James, Bartosz Kula, Seowon Choi, Shahzad S. Khan, Lane K. Bekar, Nathan A. Smith, European Journal of Neurology, November, 2020, 1-12, 10.1111/ejn.15045
- Defining the role for Vpr in HIV-1-associated neuropathogenesis., Tony James, Michael R. Nonnemacher, Brian Wigdahl, Fred C. Krebs, Journal of Neurovirology, August, 2016, 22, 4, 403-415, 10.1007/s13365-016-0436-5
- Specific amino acids in HIV-1 Vpr are significantly associated with differences in patient neurocognitive status., Will Dampier, Gregory C. Antell, Benjamas Aiamkitsumrit, Michael R. Nonnemacher, Jeffrey M. Jacobson, Vanessa Pirrone, Wen Zhong, Katherine Kercher, Shendra Passic, Jean W. Williams, Tony James, Kathryn N. Devlin, Tania Giovannetti, David J. Libon, Zsofia Szep, Garth D. Ehrlich, Brian Wigdahl, Fred C. Krebs, Journal of Neurovirology, July, 2016, 16, 106, 1-12
Timeline
Graduate Fellow/ Post-Doctoral Fellow
National Institute of Health NIH
01.2019 - Current
Research Specialist II
Johns Hopkins University School of Medicine
01.2016 - 01.2017
Associate Scientist
BioReliance
01.2015 - 01.2016
Graduate Student
Drexel University College of Medicine
01.2013 - 01.2015
Student Researcher
Arkansas State University
01.2011 - 01.2012
Student Researcher/ Assistant
Arkansas State University
01.2008 - 01.2011
PhD. - Microbiology, Immunology, and Tropical Medicine
George Washington University
Masters of Science - Microbiology and Immunology
Drexel University College of Medicine
Bachelors of Arts - Chemistry
Arkansas State University