Tyler Earp
Somerville,MA
Summary
Determined and adaptable leader with 8+ years of experience as a Scientist. Specializes in Next Generation Sequencing and Genetic Characterization. Analyzes and interprets complex data sets with a sharp and focused approach, leading to accurate and insightful conclusions. Passionate about staying updated on the latest advancements in the field to remain at the forefront of innovation.
Overview
8
8
years of professional experience
Work History
Cell Line Development Scientist III/ Group Leader
Eurofins PSS
07.2022 - Current
- Orchestrate full Genetic Characterizations of transient cell lines in CHO and compile reports for IND filings
- Perform complex troubleshooting and optimization of Next Generation Sequencing protocols and analysis of data on both the Illumina and Nanopore technologies to improve testing standards
- Utilize various molecular biology techniques to assess gross DNA and RNA structure
- Manage several complex projects simultaneously while meeting all pertinent milestones and overseeing projects of direct reports
- Train new employees on areas of technical expertise and ensure compliance with lab procedures and safety protocols
Research Technician III/Lab Manager
The University of Georgia
02.2019 - 05.2021
- Prepared libraries using multiple techniques for Next Generation Sequencing of DNA and RNA on the Illumina platform
- Organized large data files and conducted bioinformatics analysis based on the sample types and needs of the client
- Delegated tasks and trained junior team members in complex laboratory techniques and operation of equipment
- Maintained lab inventory and properly sanitized equipment to ensure an efficient work environment
Research Technician I/Mosquito Colony Manager
Pennsylvania State University
05.2017 - 05.2018
- Collaborated with the forensics department to determine efficacy of performing genetic testing of blood from the stomaches of mosquitoes at various time points
- Performed daily feeding, weekly blood meals, and brood collection of mosquito colony
- Conducted in vivo infections of mice and performing cardiac puncture blood collections for in vitro studies
- Designed plasmid cloning strategies and prepared large scale nucleic acid extractions for general lab use
Student Lab Assistant
Pennsylvania State University
08.2016 - 05.2017
- Arranged live capture traps, collected various rodents, and collected field samples
- Demonstrated skills and safety procedures to colleagues
- Prepared samples for analysis by performing complex sample preparation tasks while in the field
- Euthanized and dissected mice and organized the storage system for multiple tissue samples
Education
Bachelor of Science - Geographical Information Sciences
Arizona State University
Tempe, AZ05.2022
Bachelor of Science - Biology
The Pennsylvania State University
State College, PA05.2017
High School Diploma -
New Oxford High School
New Oxford, PA05.2012
Skills
- Next Generation Sequencing (Nanopore and Illumina)
- Transcript Analysis (RT-PCR, RT-ddPCR, and Northern Blotting)
- Bioinformatics (Python, Bash and R)
- Gene Detection (Southern Blotting)
- Copy Number Analysis (ddPCR)
- Cell Culture (E coli and CHO)
- Nucleic Acid Extraction (DNA, RNA, small RNA)
- Notebook Record Keeping (Electronic and Paper)
- Mouse Handling Techniques
- Management of Direct Reports
- Tableau and Advanced Excel Techniques
- Lab Maintenance and Procurement
Publications
Gray SL, Tiedge TM, Butkus JM, Earp TJ, Lindner SE, Roy R. Determination of human identity from Anopheles stephensi mosquito blood meals using direct amplification and massively parallel sequencing. Forensic Sci Int Genet. 2020 Sep;48:102347. doi: 10.1016/j.fsigen.2020.102347. Epub 2020 Jul 11. PMID: 32683318; PMCID: PMC7487007.
Timeline
Cell Line Development Scientist III/ Group Leader
Eurofins PSS
07.2022 - Current
Research Technician III/Lab Manager
The University of Georgia
02.2019 - 05.2021
Research Technician I/Mosquito Colony Manager
Pennsylvania State University
05.2017 - 05.2018
Student Lab Assistant
Pennsylvania State University
08.2016 - 05.2017
Bachelor of Science - Geographical Information Sciences
Arizona State University
Bachelor of Science - Biology
The Pennsylvania State University
High School Diploma -
New Oxford High School
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