Summary
Overview
Work History
Education
Skills
Websites
Timeline
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TYLER J. HINTHORN

Joliet,IL

Summary

Biomedical scientist specializing in molecular biology, immunology, and high-dimensional immune profiling (CyTOF/flow cytometry). Published in cardiovascular and immunology research with expertise in biomarker discovery, protein/RNA analysis, and translational assay development. Proficient in FlowJo, R/Python, and GraphPad within GLP/CLIA-regulated environments. Known for delivering accurate, reproducible datasets and streamlining complex lab workflows to accelerate diagnostics and therapeutic development.

Overview

3
3
years of professional experience

Work History

Graduate Research Intern

Human Immune Monitoring Shared Resource
07.2024 - 12.2024
  • Mapped immune activation profiles in PBMC, T cell, and monocyte subsets from post-9/11 veterans via CyTOF, uncovering biomarkers (CD14+, CD11b+, HLA-DR+) associated with burn pit–linked respiratory disease.
  • Applied FlowJo and CyTOF XT analytics to quantify immune marker expression and cytokine networks, streamlining QC and processing workflows to boost multi-batch data accuracy.
  • Integrated multi-omics datasets with clinical metadata to inform biomarker validation and support translational research in respiratory and immune-mediated disorders.

Laboratory Science Professional

University of Colorado Anschutz Medical Campus
01.2023 - 08.2024
  • Mapped smooth muscle and adventitial progenitor cell (AdvSca1-SM) reprogramming in murine vascular injury models, identifying therapeutic targets for ischemic revascularization.
  • Defined PTEN-regulated pathways influencing SMC differentiation and atherosclerosis progression, guiding experimental design in vascular biology.
  • Delivered high-quality preclinical datasets by optimizing PCR, Western blotting, genotyping, breeding, and fate-mapping workflows, boosting data throughput by ~20%.

Laboratory Intern

City of Joliet Wastewater and Drinking Water Laboratory
05.2022 - 10.2022
  • Performed EPA-compliant testing for ammonia, BOD, fecal coliform, phosphorus, and TSS to ensure regulatory compliance.
  • Conducted field sampling and water quality analysis, enhancing data accuracy and reporting efficiency.

Education

Master of Science - Biomedical Sciences and Biotechnology

University of Colorado Anschutz Medical Campus
Aurora, CO
12.2024

Bachelor of Science - Biology

University of St. Francis
Joliet, Illinois
12.2022

N/A - Exercise Science And Physiology (Direct Admit PT)

Saint Louis University
St. Louis, MO

Skills

  • Flow Cytometry & Mass Cytometry (CyTOF)
  • Immunohistochemistry (IHC)
  • Protein & RNA Analysis
  • Biomarker Discovery & Validation
  • PCR / qPCR
  • Western Blotting
  • Next-Generation Sequencing (NGS) & Bioinformatics
  • Disease Modeling (Cardiovascular, Oncology, Immunology)
  • LC/MS Analysis
  • Histology & Tissue Processing
  • GLP / CLIA Compliance
  • Data Analysis (FlowJo, R, Python, GraphPad Prism)

Timeline

Graduate Research Intern

Human Immune Monitoring Shared Resource
07.2024 - 12.2024

Laboratory Science Professional

University of Colorado Anschutz Medical Campus
01.2023 - 08.2024

Laboratory Intern

City of Joliet Wastewater and Drinking Water Laboratory
05.2022 - 10.2022

Master of Science - Biomedical Sciences and Biotechnology

University of Colorado Anschutz Medical Campus

Bachelor of Science - Biology

University of St. Francis

N/A - Exercise Science And Physiology (Direct Admit PT)

Saint Louis University

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