Highly motivated candidate who has expertize in tissue culture, cancer biology and RNA biology.
Characterization of CD20 expression loss as a mechanism of resistance to mosunetuzumab in patients with relapsed/refractory B-cell non-Hodgkin lymphomas
Generated CD20 mutant clones using CRISPR-Cas9, Validated the expression of CD20 mutant expression using qPCR and Immunohistochemistry, Tested the mechanism of resistance to mosunetuzumab in these CD20 mutant clones using killing assay and flow cytometry.
Validate the binding affinity of novel antibodies against immune check point proteins
Duties include maintenance and processing of cell cultures, transient expression of immune check point markers in vitro cancer cell lines, validating their expression using techniques such as flow cytometry, ELISA, Study the binding affinity of antibodies designed against these check point proteins.
Human cell line development and process development, Bio-production of proteins using fed-batch bioreactors.
Reggiardo, R.E., Maroli, S.V., Peddu, V. et al. Profiling of repetitive RNA sequences in the blood plasma of patients with cancer. Nat. Biomed. Eng (2023)
Roman E. Reggiardo, Sreelakshmi Velandi Maroli, et al. Mutant KRAS regulates transposable element RNA and innate immunity via KRAB zinc-finger genes,Cell Reports, Volume 40, Issue 3, 2022, 111104, ISSN 2211-1247)
Sabapathy V, Sundaram B, VM S, Mankuzhy P, Kumar S (2014) Human Wharton’s Jelly Mesenchymal Stem Cells Plasticity Augments Scar-Free Skin Wound Healing with Hair Growth. PLOS ONE 9(4): e93726
Khojah, R, Reggiardo, R.E., Ozen, M, Maroli, S.V. et al. Extracellular RNA signatures of mutant KRAS(G12C) lung adenocarcinoma cells (Pre-print)
Influence of KRAS on transposable element RNA during stem cell differentiation (Thesis project)
Used embryoid body model to identify how KRAS regulate transposable element derived non coding RNAs during exit stem cell differentiation. Used a combination of RNA-seq, nanopore-native RNA-seq, and single cell RNA-seq platform to interrogate the transcriptional consequences of the lack of KRAS signaling in human induced pluripotent stem cells during pluripotency and differentiation.
Profiling of repetitive RNA sequences in the blood plasma of patients with cancer
Reggiardo, R.E., Maroli, S.V., Peddu, V. et al. Profiling of repetitive RNA sequences in the blood plasma of patients with cancer. Nat. Biomed. Eng (2023).
Here we showed that RNAs derived from transposable elements and other repeat elements are enriched in the cell-free transcriptome of patients with cancer, and that they serve as signatures for the accurate classification of the disease. My role involved the isolation and characterization of extra cellular vesicle derived RNA from clinical cancer patients, ultra-low input RNA-seq library preparation and sequencing.
Mutant KRAS regulates transposable element RNA and innate immunity via KRAB zinc-finger genes
Roman E. Reggiardo, Sreelakshmi Velandi Maroli, et al. Mutant KRAS regulates transposable element RNA and innate immunity via KRAB zinc-finger genes,Cell Reports, Volume 40, Issue 3, 2022, 111104,
ISSN 2211-1247)
Here we show that mutant KRAS regulates the RNA landscape by silencing KRAB zinc-finger genes that normally repress transposable elements non coding RNAs, which are preferentially released from mutant KRAS cells in extracellular vesicles. My role involved characterization of the ISG signaling in mutant KRAS cancer cell lines, isolation of extracellular and intracellular RNA, bulk RNA-seq library prep and ATAC-seq library prep.
Human Wharton’s jelly mesenchymal stem cells plasticity augments scar-free skin wound healing with hair growth
Sabapathy V, Sundaram B, VM S, Mankuzhy P, Kumar S (2014) Human Wharton’s Jelly Mesenchymal Stem Cells Plasticity Augments Scar-Free Skin Wound Healing with Hair Growth. PLOS ONE 9(4): e93726
WJ-MSCs seeded on decellularized amniotic membrane scaffold transplantation on the skin injury of SCID mice model exhibited significantly better wound-healing capabilities, having reduced scar formation with hair growth and improved biomechanical properties of regenerated skin. My role in this project involved the entire characterization of WJ-MSCs including flow cytometry, differentiation potential, cell cycle analysis and in vivo imaging using IVIS imaging station.
Extracellular RNA signatures of mutant KRAS (G12C) lung adenocarcinoma cells (Pre-print)
Here we used both affinity- and nanofiltration-based extracellular vesicle RNA (EV RNA) isolation approaches to show that mutant KRAS(G12C) signaling results in the secretion of specific lncRNAs, transposable element RNAs (TE RNA), and mRNAs, some of which are prognostic for lung adenocarcinoma (LUAD) patient survival. We found that inhibition of KRAS(G12C) signaling broadly reprograms the noncoding transcriptome, as evidenced by a substantial increase in TE RNA secretion. KRAS(G12C) inhibition also increased the abundance of secreted lncRNAs and retained intron-containing transcripts, while decreasing the mRNA content of EVs. My role invovled the tissue culure of lung adenocarcinoma cells, optimization of KRASG12C inhibitor concentration to inhibit mutant KRAS and library prep of the total RNA isolated from EV RNA.