Kathryn Sheldon
Seattle,WA
Summary
Scientist with advanced experience in research methodologies, data analysis, and driving projects. Proficient in laboratory techniques, analysis software, and experimental design. Known for reliability, problem-solving skills, and effective communication in collaborative environments.
Overview
14
14
years of professional experience
Work History
Research Scientist 2
Department of Medicine, University of Washington
08.2022 - Current
- Cell culture, transfection, transduction, and flow analysis related to novel Deep Mutational Scanning projects
- PCR recreation of specific genetic mutations via IVA cloning
- Physical and digital maintenance of a large human sample biorepository of over 9,000 patients
- Phenotypic and functional analysis of protein mutations using multi-parameter flow cytometry
Research Technologist II (Johnsen Lab)
Bloodworks Northwest
03.2019 - 08.2022
- Operation and regular upkeep of a QIAsymphony instrument for high-throughput RNA extractions of patient samples in a molecular clean room environment
- Analysis of RNA sample quantity and quality, patient sample workflow tracking, and data entry using Progeny Genetics LIMS software
- Optimization of membrane-based assays for accurate and quantitative detection of human blood-clotting proteins
Research Technologist I (Hudson Lab)
Bloodworks Northwest
08.2017 - 03.2019
- Maintaining a colony of approximately 20 distinct congenic and transgenic mutant murine strains through careful selective breeding and basic animal husbandry
- Regular phenotyping and genotyping of individual weanlings, using red and white blood cell screening techniques and PCR
- Investigating the mechanisms of red blood cell alloimmunization through isolating specific immune cells by type and function, staining cells with fluorescent lipophilic dyes, and transfusing to track immune responses over time
Lab Assistant
Evergreen Sciences
06.2015 - 08.2017
- Responsible for reagent preparation, manufacturing QC, general lab upkeep, and waste management
- Developed and manufactured ELISA test products to specific parameters
- Used Ridasoft and Excel for data analysis, and Quartzy for inventory control and SDS management
- Trained and supervised new employees
- Maintained lab by calibrating and repairing equipment and lab instruments
Barista
Starbucks
03.2015 - 06.2015
- Prepared beverages with great attention to small details to suit customers' tastes and diets
- Handled all food and drink items according to strict HACCP and food safety guidelines
- Responded to and anticipated needs with clear communication to avoid and repair errors
- Maintained the cafe by washing dishes and machines, and preparing beverage ingredients
Team Member
Jo-Ann Stores, LLC.
06.2014 - 06.2015
- Engineered project plans and solutions suited to customers' ideas or problems
- Used previous art experience and chemical knowledge to recommend the most suitable materials and adhesives for varying projects
- Maintained store organization and approachable demeanor while multitasking heavily and helping with queries
Language Mentor
ACE Language Institute
03.2011 - 12.2014
- Facilitated international students' adjustment to school and life in the U.S.
- Engaged students actively while tutoring to improve classwork performance
- Assisted several students in preparing for final IELTS exams, with excellent results
- Acted as a liaison, bridging cultures and fostering mutual understanding
Education
Bachelor of Science - Biochemistry
Seattle Pacific University
Seattle, WA06-2014
Skills
- Data analysis
- Technical troubleshooting
- Experiment design
- Cell Culture
- Molecular Biology techniques
- Sample Biorepository and LIMS stewardship
Languages
English (Native or Bilingual)
Spanish (Professional Working)
Limited Working
Timeline
Research Scientist 2
Department of Medicine, University of Washington
08.2022 - Current
Research Technologist II (Johnsen Lab)
Bloodworks Northwest
03.2019 - 08.2022
Research Technologist I (Hudson Lab)
Bloodworks Northwest
08.2017 - 03.2019
Lab Assistant
Evergreen Sciences
06.2015 - 08.2017
Barista
Starbucks
03.2015 - 06.2015
Team Member
Jo-Ann Stores, LLC.
06.2014 - 06.2015
Language Mentor
ACE Language Institute
03.2011 - 12.2014
Bachelor of Science - Biochemistry
Seattle Pacific University
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