I am a detail-oriented Graduate Research Assistant with hands-on experience performing agronomy and laboratory work to advance research goals. Highly proficient in conducting comprehensive literature reviews, providing computational research support and preparing samples and specimens for testing. Currently I am pursuing a doctorate degree in Entomology and Plant Pathology with a concentration in Sustainable Disease and Integrated Pest Management Systems. I look forward to completing my degree and work in the agriculture industry and get to practice and utilize skills learnt, and help transform the sector.
I am currently working on two organic projects where one we seek to improve indigenous soil microflora, through use of anaerobic soil disinfestation.in strawberry fields. The other project we working on a system that is farmer friendly and involves grain rotations in organic fields in a way that optimizes agronomic, economic, and ecological outcomes in Tennessee
I was involved in sampling genetically engineered plant tissue for DNA and protein testing in search of desired traits, monitoring and maintenance of cotton trait introgression production plants.
I worked in a lab where I was involved in tissue culturing, mentoring undergraduates and high school students who worked in the lab. I worked on several projects such as Improving bioenergy lignocellulosic feedstock through CRISPR-Cas9 technology in switchgrass (Panicum virgatum L.) and also Improving seed size in soybean
I was responsible in visual diagnosis of insect pests and diseases. Prescription of Pesticides against target pests, supervision of pesticide mixing as well as sprays . Keeping records of all pesticide applications
I was actively involved in the formulation and implementation of policies governing sustainable development of the floriculture sector in Kenya. Attended 12 audits in different flower farms in Kenya and conducted audits on good agricultural practice, human resource management and workers welfare, health & safety, environmental protection & conservation, and post-harvest management
Was involved in growing of crops for the children's home farm, fencing of the home as well as landscaping processes
Lab Skills- Tissue culture, PCR, Gel electrophoresis, Cloning, Transformation and Regeneration of plants
Data analysis with SAS,R, SPSS, Graphpad Instat, JMPin softwares
Computer skills: Databases, HTML,JavaScript, Ms Office, MySQL, FAOStat Locclim, Aquastat
Lab Management
Publications
1. Christine A. Ondzighi-Assoume, Jonathan D. Willis, Wilson K. Ouma, Sara M. Allen, Zachary King, Wayne A. Parrott, Wusheng Liu, Jason N. Burris, Scott C. Lenaghan, and C. Neal Stewart, Jr. (2019). Embryogenic Cell Suspensions for High Capacity Genetic Transformation and Regeneration of Switchgrass (Panicum virgatum L.) (Submitted to Biotechnology for Biofuels)
2. Wilson K. Ouma, Margaret T. Mmbaga, C. Korsi Dumenyo, Matthew Blair, and Christine A. Ondzighi-Assoume. (2020). Mutagenesis of Peroxidase Genes through CRISPR-Cas9 Technology Reveals a Decrease in Lignin Contents in Switchgrass (Panicum virgatum L.). (In preparation)
Commercial Pesticide Applicator C03 – Ornamental and Turf
Gardening, Playing soccer and chess, visiting new places
1.Dr. Bonnie H. Ownley,
Professor/ Research Plant Pathologist
Assistant Department Head/ Director of Graduate Studies
Department of Entomology and Plant Pathology The University of Tennessee
370 Plant Biotechnology, 2505 E.J. Chapman Drive
Tel: 865-974-0219
bownley@utk.edu
2. Bharat Pokharel
Assistant Professor of Applied Statistics and Graduate Coordinator
Department of Agricultural and Environmental Sciences College of Agriculture Tennessee State University Nashville, TN 37209
Tel: (615) 963-6054
bpokhare@TNState.edu
3. Dr. Christine A. Ondzighi-Assoume,
Department of Agricultural & Environmental Sciences
College of Agriculture Tennessee State University 3500 John A. Merritt Blvd.
Nashville, Tennessee 37209, USA Tel: 615-963-6334
condzigh@tnstate.edu