Yohannes Abere Ambaw
Bridgeport
Summary
Over10 years of research experience in bio-analytics, with a special focus on method development, validation, and implementation for bioanalysis. Trained in cutting edged LC-MS/MS, and shotgun-based analysis of small molecule and large molecule, as well as molecular biology and biochemical techniques. Experienced in leading analytical unit, establishing automated pipelines, maintaining rigorous quality control processes, and conducting clinical research. Experienced in biomarker discovery and translation, precision medicine, and clinical trial planning. A detail-oriented scientist with exceptional task prioritization and execution skills.
Overview
5
5
years of professional experience
Work History
POSTDOCTORAL RESEARCH ASSOCIATE
Memorial Sloan Kettering Cancer Center, Sloan Kettering Institute, Cell Biology Department
12.2022 - 09.2024
• Developed, validated, and implemented various LC-MS/MS methods for lipids, oligonucleotides, and peptides.
• Identified and structurally characterized new analytes.
• Build an open-source web application for data analysis.
• Analysis, interpretation, and communication of pre-clinical and clinical datasets
• Maintained rigorous quality control (QC) and quality assurance.
• Managed the operation and maintenance of instruments.
• Led projects focused on identifying potential plasma biomarkers and therapeutic targets for neurodegenerative diseases (FTD, AD) and cancer using multi-omics analysis.
POSTDOCTORAL RESEARCH FELLOW
Harvard T. Chan School of Public Health & Harvard Medical School
01.2020 - 11.2022
- • Established various LCMS methods for small molecules.
• Established an automated sample preparation workflow.
• Characterized and defined human plasma lipidome in cardiometabolic diseases.
• Identified lipid/sphingolipid abnormalities in the lysosomes as an underlying disease-causing factor in frontotemporal dementia (FTD)
Education
Ph.D. - Analytical chemistry and Biochemistry
Yong Loo Lin School of Medicine, National University of Singapore
01.2019
MSc - Medical Biochemistry
Addis Ababa University
01.2011
BSc - Applied Chemistry
University of Gondar
01.2007
Skills
- Bioanalysis, Lipidomic, HPLC, Mass spectrometry, Metabolomics, Oligonucleotides, Proteomics analysis, LC-MS/MS, shotgun, GCMS, NMR, FTIR, UV-VIS, SPE and LLE sample processing, Biomarker discovery, Data analysis, Clinical research, Derivatization, Chiral separation, Metabolic tracing, Radiolabeling, Protein precipitation, Size exclusion chromatography, Cell culture, Western blots, ELISA, qPCR
- Well experienced in operating various mass spectrometry and HPLC systems (Thermo-Orbitrap (QE, QE-HF, OE240, OE480, IDX), Agilent (6491,6495 QQQ, TOF), Waters, Bruker, and Sciex (AB Sciex, QTrap) and its software
- Proficient in using office software, Word, Excel, PowerPoint, Prism, illustrator, RStudio
Awards
2023 Bluefield postdoctoral fellowship award for curing Frontotemporal dementia (FTD), Fellowship Singapore international national graduate award (SINGA)
Timeline
POSTDOCTORAL RESEARCH ASSOCIATE
Memorial Sloan Kettering Cancer Center, Sloan Kettering Institute, Cell Biology Department
12.2022 - 09.2024
POSTDOCTORAL RESEARCH FELLOW
Harvard T. Chan School of Public Health & Harvard Medical School
01.2020 - 11.2022
MSc - Medical Biochemistry
Addis Ababa University
BSc - Applied Chemistry
University of Gondar
Ph.D. - Analytical chemistry and Biochemistry
Yong Loo Lin School of Medicine, National University of Singapore
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