Shruthi Seetharaman
CAMBRIDGE,MA
Summary
Research investigator with a 4 year experience in the field of cell therapies. Demonstrated expertise in developing multi-parameter flow cytometer assays and executing cell based immunoassays which including potency and molecular assays. I have worked majorly on T cell and NK cell therapy platforms. Expertise in operating various cytometers including BD FACSLyric, Canto. Collaborated across cross functional teams and ability to work under pressure.
Overview
10
10
years of professional experience
Work History
Research Investigator /Associate Scientist
Takeda Pharmaceuticals
CAMBRIDGE, MA
05.2021 - Current
- Designed & developed high parameter immunophenotype panels on BD FACSlyric for characterization of starting material and DP for (Chimeric Antigen Receptor) CARNK and Gamma delta cell therapies platform.
- Optimized BD Lyric by performing voltage titration for immunophenotype assay development.
- Prequalified immunophenotype assay to establish the robustness, precision and specificity for DP release and collaborated with cross functional teams for tech transfer and routine testing.
- Optimized and validated the immunophenotype assays and performed fit for purpose assay validatation.
- Designed Flourescne minus multiple (FMM) in place of FMO controls for feasabiility of tech transfer.
- SME for flow cytometry in the AD department.
- Executed infectious titer assays to assess transduction efficiency and to quantitate CAR expression.
- Executed cytotoxicity assays to assess the potency of CARNK DP.
- Conducted proliferation assays aimed at assessing the proliferation rate of NK cells.
- Executed surface vs intracellular staining to compare the CAR expression in DP vs UTD.
- SME for critical reagent testing which included in house generated anti CAR antibody clones to identify best clone for CAR detection.
- Analyzed the data using analytical tools Flowjo, Graphpad Prism, FACSuite, excel
- Performed cell culture to maintain cell lines utilized in cytotoxicity studies. Banked cells like raji, K562, daudi cells and made Master Cell Bank (MCB) and Working Cell Bank (WCB) using Cryomed freezer.
- SME for stability testing for determining the stability of DP overtime by testing at different timepoints for its appearance, extraction volume, cell count and viability, immunophenotype.
Research Associate
RUBIUS THERAPEUTICS
CAMBRIDGE, MA
11.2020 - 05.2021
- Gained insights into cell stability, collaborated with formulation group, mastered buffer preparation, and executed cryopreservation studies with Cryomed freezers.
- Stimulated innate and adaptive immune responses by utilzing engineered Red Blood Cells (RCTs).
- Performed assays like Counts & Purity, CD233, Viability by Calcein, Complete Blood Count (Sysmex), determine Potassium Concentration, Free Hemoglobin, ATP.
- Utilized microplate readers for ELISA and other Cell based assays.
- Performed survival/fitness studies to determine the performance of cells in physiological conditions.
- Utilized Benchling Notebook and Spotfire for thorough data analysis and reporting, effectively communicating findings to larger audiences during team meetings.
Research Intern
CELGENE
NJ
06.2019 - 08.2019
- Explored optimization of ELISA process by doing Sandwich ELISA and Direct ELISA and learnt several skills including serial dilution, forward & reverse pipetting
- Conducted various experiments to determine the most efficient ELISA protocol to create our ELISA plates.
- Used different concentrations of nickel to optimize plate coating for product specific ELISA.
- Increased the efficiency of the assay by reducing the total time for each plate.
University Research
RUTGERS UNIVERSITY
NJ
01.2014 - 01.2018
- Worked on a research paper with my professor on oncolytic virotherapy focusing on clinical developments using techniques for minimal sequestration in liver & spleen.
- Focused on methods to hide oncolytic viruses using mesenchymal stem cells to deliver viruses to tumor site.
- Research focused on melanoma, breast cancer & pancreatic cancer and specific viruses that treat them.
- Performed DNA Isolations, PCR and Gel Electrophoresis.
- Participated in Animal Study which dealt with study of alcohol consumption of pregnant female mice to analyze the resultant possibility of Breast Cancer.
- Worked in an Animal Science Lab. Learnt how to Gavage mice and learnt their life cycle.
- Used insects in an Entomology lab to study their patterned behaviors using Light Study while preparing field studies and managing insect cages periodically.
Education
MS Biotechnology - MTDD (Molecular Targets and Drug Discovery) & Enterprise
JOHNS HOPKINS UNIVERSITY
05.2020
BS Biological Sciences -
RUTGERS UNIVERSITY
05.2018
Skills
- Flow Cytometry : Surface and Intracellular staining, FlowJo, FACSuite, GraphPad Prism
- Cytotoxicity assay
- Immunoassays : ELISA, Chemiluminescent
- Infectious titer
- Cell culture
- Molecular Assays : DNA Isolations, PCR, Gel Electrophoresis
- R Studio
- MS Office : Excel, PowerPoint, Word
- Adaptability and Flexibility
- Presentation and Pitching
Coursework And Specialization
- Chemical Libraries & Diversity (Lipinski’s Rules, in silico drug design, molecular modeling, PK/PD)
- High-Throughput Screening Lab
- Bioinformatics, Biostatistics
- Translational Biotechnology – IP to Licensing, Ethical and Legal aspects of Biotechnology
Awards
- Dean’s List – Rutgers University, 2013-2017
- Nominated for George. H Cook Scholars Program, 2016, 2017
- Immunology Course – Harvard University, 2023
Timeline
Research Investigator /Associate Scientist
Takeda Pharmaceuticals
05.2021 - Current
Research Associate
RUBIUS THERAPEUTICS
11.2020 - 05.2021
Research Intern
CELGENE
06.2019 - 08.2019
University Research
RUTGERS UNIVERSITY
01.2014 - 01.2018
MS Biotechnology - MTDD (Molecular Targets and Drug Discovery) & Enterprise
JOHNS HOPKINS UNIVERSITY
BS Biological Sciences -
RUTGERS UNIVERSITY
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