Aishwarya Ramanujan
Waltham
Summary
Positive, adaptable, and analytical with strong ability to thrive in fast-paced setting.
Brings attention to detail, producing accurate and quality work.
Poised to contribute to team success, ready to tackle new challenges and advance organizational objectives with dedication and enthusiasm.
Overview
5
5
years of professional experience
Work History
Cell Manufacturing Associate
Editas Medicine
02.2023 - Current
- Manufacturing cellular products as directed by the standard operating procedures under GxP guidelines
- Working in a clean room environment using aseptic techniques and in Biosafety cabinets for open processing operations
- Operating instruments (LOVO, CliniMacs, Sepax, Maxcyte) for cell product manufacturing
- Regularly performing cell selection and electroporation processes
- Maintaining zero safety incidents and minimum operator deviations
- Completing and reviewing SOPs and batch record documentation
- Working with QA to implement CAPAs, CCs and deviations
- Actively involved with team to resolve manufacturing-related issues/challenges
Senior Research Associate
Editas Medicine
11.2021 - 01.2023
- Developed and executed in vitro cell-based potency assays to evaluate natural killer cells
- Characterized phenotype of immune cells using flow cytometry
- Analyzed and drew correlation between functional and phenotypic readouts using Prism and Tableau
- Utilized the Biomek for improved throughput of in-vitro assays
- Collaborated with the Analytical development team to transfer assays
Research Associate
Cygnal Therapeutics
01.2021 - 10.2021
- Cultured Murine and Human cancer cell lines to support in-vivo studies
- Performed lentiviral based transfection to support target ID efforts by generating stable cell
- Assessed transfection efficiency using flow cytometry
- Examined functional blockade of signaling pathways with antibodies of interest using an ELISA
- Isolated bone marrow cells from mice models for gene knock-out studies in macrophages
Laboratory Teaching Assistant
Northeastern University
08.2020 - 12.2020
- Cultured E.Coli K12: LB plate streaking and LB broth inoculation, transformation with GFP plasmid (pGLO)
- Conducted BCA assay, SDS PAGE, agarose gel electrophoresis, western blotting, DNA extraction, HIC for protein purification
- Maintained lab inventory and lab records
- Provided academic support to the students and graded paperwork
Education
Master of Science - Biotechnology; Pharmaceutical Technologies
Northeastern University
08.2021
Skills
- Mammalian & Bacterial Cell Culturing
- Co-Culturing
- Cell Killing Assays
- Editing Potency Assays
- ELISA
- MSD
- GMP documentation
- Aseptic techniques
- ISO 5,7,8 gowning
- LOVO
Timeline
Cell Manufacturing Associate
Editas Medicine
02.2023 - Current
Senior Research Associate
Editas Medicine
11.2021 - 01.2023
Research Associate
Cygnal Therapeutics
01.2021 - 10.2021
Laboratory Teaching Assistant
Northeastern University
08.2020 - 12.2020
Master of Science - Biotechnology; Pharmaceutical Technologies
Northeastern University
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