Summary
Overview
Work History
Education
Skills
LANGUAGES
Publications
RESEARCH INTEREST
PERSONAL STATEMENT
Timeline
Generic
Ajitanuj Rattan

Ajitanuj Rattan

Boston,USA

Summary

A versatile and enthusiastic researcher with more than 6-years of post-doctoral research experience in Immunology, autoimmunity, vaccine design and drug discovery. More than 15 years of multidisciplinary and translational research experience. Demonstrated experience of working in collaborative projects, ability to work as a team. Keen interest in translational research (Immunotherapy, vaccine design), entrepreneurship, business development and science communication.

Overview

16
16
years of professional experience

Work History

Senior Research Scientist

Boston Children's Hospital
03.2025 - Current
  • specialize in empowering scientific discovery by managing and optimizing flow cytometry resources as both a technical expert and strategic partner.
    As Senior Research Scientist, I now oversee the Flow Cytometry Core Facility, where I combine hands-on instrumentation mastery with operational leadership to elevate research outcomes across diverse disciplines.
    Lead daily operations of a high-throughput flow core, ensuring peak instrument performance (BD FACS ARIA, Cytek Aurora, ImageStream, Sony SH800S) and user compliance with biosafety protocols.
    Guide scientists and researchers in experimental design, panel configuration, and advanced applications (e.g., spectral cytometry, complex cell sorting) to accelerate project success.

Post-Doctoral Research associate

Boston Children’s Hospital, HMS
02.2020 - Current
  • Research highlights: -
  • To explicate the role of complement receptor CD21, FDC and macrophages in auto B cell tolerance and development of autoimmunity.
  • Developed an assay for apoptotic cell clearance by MΦs to elucidate the role of a newly identified complement receptor in autoimmunity and its regulation. By using in-vivo conditional knock out mouse strain of the receptor protein, we established the role of this protein in augmenting MΦs capacity to clear apoptotic cells.
  • Current project involves humanized mouse model to investigate FDC requirement and CD21 mediated signaling on B cell or the maintenance and survival of auto B cells, using RNA seq, antibody cloning, Flow cytometry and confocal microscope.

Post-Doctoral Fellow

URMC, University of Rochester
01.2017 - 03.2020
  • Research highlights: -
  • Infection and vaccination:
  • Optimized the protocol for expression and purification of viral recombinant protein used in vaccination studies. Evaluated and compared CD4 T cell specificities generated after protein vaccination and infection in different mice strains by employing a heterologous protein vaccination system and infection challenge experiments, using a reverse-engineered influenza A virus (Rattan et al, JVI, 2019).
  • Mouse model of Influenza B virus infection:
  • Established a mouse model to evaluate the kinetics, phenotype, and effector functions of CD4 T cells, antibody response to influenza B virus infection by EliSpot, ELISA and flow cytometry in comparison with influenza A virus (Rattan et al, Pathogen, 2022)
  • Virus propagation and purification by ultra-centrifugation, assay development for in-vitro virus neutralization and to quantify complement deposition on virus surface.
  • Chimeric vaccine construct:
  • In a collaborative work, contributed equally to evaluate the potential role of a novel chimeric HA protein vaccine to overcome the poor immunogenicity of avian vaccines using flow cytometry, EliSpot and ELISA (DiPiazza and Rattan et al, JI, 2019).

Ph.D.

NCCS Complex
11.2009 - 11.2016
  • Research highlights: -
  • Established the relative role of individual complement pathway(s) (classical/lectin and alternative) and their ability to instruct and enhance acquired immune responses against the pandemic influenza virus by doing comparative infection studies in different complement null mice (C3-/-, C4-/- and FB-/-) using a mouse model of pandemic influenza A(H1N1) pdm09 virus infection (Rattan et al, Plos Pathogen, 16;13(3); p1-26, 2017).

Education

Ph.D. - Biotechnology

National Center for Cell Science (NCCS)
01.2016

M.Sc. - Biotechnology

Guru Nanak Dev University
01.2009

B.Sc. - undefined

Himachal Pradesh University
01.2006

Skills

  • Skilled in flow cytometry analysis with Cytek systems
  • Single-cell and bulk RNA analysis
  • Expertise in confocal microscopy techniques
  • T/B-cell assay design
  • ELISA, ELISPOT
  • Antibody Cloning

LANGUAGES

English - Professional
Hindi - Native

Publications

  • Rattan A, Danni Z, Kristine O, Carroll MC. CD21 Blockade on B cells and FDC impede the capacity of B cell to drive generation of auto antibody secreting B cell (ASC) in mouse model of Systemic Lupus Erythematosus (SLE). (MS in preperation).
  • Rattan A, White CL, Nelson S, Eismann M, Padilla-Quirarte H, Glover MA, Dileepan T, Marathe BM, Govorkova EA, Webby RJ, Richards KA, Sant AJ (2022). Development of a Mouse Model to Explore CD4 T Cell Specificity, Phenotype and Recruitment to the Lung after Influenza B Infection. Pathogens. 11(2):251. https://doi.org/10.3390/pathogens11020251
  • Rattan A, Richards KA, Knowlden Zackery AG and Sant AJ (2019). Protein vaccination directs the CD4 T cell response towards shared protective epitopes that can be recalled after infection. J. Virol. 93 (20): e00947-19.
  • DiPiazza AT, Fan S, Rattan A, DeDiego ML, Chaves F, Neumann G, Kawaoka Y, and Sant AJ (2019). A novel vaccine strategy to overcome poor immunogenicity of avian influenza vaccines through mobilization of memory CD4 T cells established by seasonal influenza. J. Immunol. 203(6), 1502-1508. 'All authors contributed equally (first co-authors).
  • DiPiazza AT, Laniewski N, Rattan A, Topham DJ, Miller J, Sant AJ (2018). CD4 T Cell Epitope Specificity and Cytokine Potential Are Preserved as Cells Transition from the Lung Vasculature to Lung Tissue following Influenza Virus Infection. J. Virol. 92(13), e00377-18.
  • Richards KA, DiPiazza AT, Rattan A, Knowlden ZAG, Yang H, Sant AJ (2018). Diverse Epitope Specificity, Immunodominance Hierarchy, and Functional Avidity of Effector CD4 T Cells Established During Priming Is Maintained in Lung After Influenza A Virus Infection. Front Immunol. (9), 655.
  • Sant AJ, DiPiazza AT, Nayak JL, Rattan A, Richards KA. (2018). CD4 T cells in protection from influenza virus: Viral antigen specificity and functional potential. Immunol Rev. 284(1), 91-105.
  • Rattan A, Pawar SD, Nawadkar R, Kulkarni N, Lal G, Mullick J, and Arvind Sahu A. (2017). Synergy between the classical and alternative pathways of complement is essential for conferring effective protection against the pandemic influenza A (H1N1) 2009 virus infection. Plos Pathogen, 16;13(3); p1-26.

RESEARCH INTEREST

I have keen interest in translational research, specifically in drug discovery and Immunotherapy. Looking for opportunities where I can apply my knowledge and expertise to advance the clinical and preclinical research, design therapeutic tools to tackle novel emerging viruses, cancer, and autoimmunity.

PERSONAL STATEMENT

  • Looking for new opportunities to:
  • Translate my training, knowledge, and expertise in basic science to advance the clinical and preclinical research, design therapeutic tools to tackle novel emerging viruses, cancer, and autoimmunity.
  • Next step in career development together with the resource to pursue my passion for innovation and advancement of research in immunotherapy and vaccine development.

Timeline

Senior Research Scientist

Boston Children's Hospital
03.2025 - Current

Post-Doctoral Research associate

Boston Children’s Hospital, HMS
02.2020 - Current

Post-Doctoral Fellow

URMC, University of Rochester
01.2017 - 03.2020

Ph.D.

NCCS Complex
11.2009 - 11.2016

M.Sc. - Biotechnology

Guru Nanak Dev University

B.Sc. - undefined

Himachal Pradesh University

Ph.D. - Biotechnology

National Center for Cell Science (NCCS)