Amalea Misse
Rockville,MD
Summary
Experienced Research Associate with 15+ years of data collection, analysis, and interpretation expertise. Known for strong analytical skills that drive project success. Proficient in designing research methodologies, conducting experiments, and presenting findings to enhance strategic decision-making. Made significant contributions to improving operational efficiency through comprehensive research efforts.
Overview
16
16
years of professional experience
Work History
Senior Research Associate
PRECIGEN Inc
Germantown
11.2018 - Current
Research Associate
Department of Neurology & Pharmacology (GWU)
01.2017 - 01.2018
- Therapeutics Assessment and monitoring
- ELISA
- Immunohistochemistry (Fluorescence) and Histology
- Data analysis via confocal microscopy
- Cell Culture
- Animal experiments (Rats and Mice)
Department of Critical Care Medicine- (The Safar Center)
01.2011 - 01.2017
- Adult rat Asphyxial (ACA) and Ventricular Fibrillation
- (VF) Cardiac Arrest models
- Placement of electrocardiographic leads to the limbs and endotracheal intubation
- Cannulation of femoral vein and artery
- Blood gas analysis
- Intravenous injections (Vecuronium, Sodium Bicarbonate, Epinephrine, normal saline)
- Mechanical ventilation and chest compression, after asphyxia cardiac arrest
- Implantation of Mini mitter( E-mitter) and monitoring of core body temperature and activity
- Craniotomy
- Measure of the cerebral blood flow (with Laser spectral and MRI) and cardiac output
- Neurobehavioral testing (Fear conditioning, Neuro Deficit Score…)
- Treatments such Nitrite or others drugs Pre and post resuscitation or intra-CPR
- Animal sacrifice by perfusion (using Para-formaldehyde, Saline and PFA)
- Tissue dissection and collection, mostly brain (Hippocampus, Cortex, Cerebellum…), heart and plasma collection
- For Western Blotting
- For Histology
- For Enzyme activity assays: Amplex Red, Aconitase, Caspase, Calpain
- Mitochondrial isolation from Brain/Heart and Mitochondrial respiration assay with Oxygraph
- Primary Culture of Neurons (Cortex) from embryonic rats
- Oxygen and Glucose Deprivation
- Using Live cell imaging microscope with cells pre-labelled with MitoSox and JC-1
- Using Hypoxia chamber
- Using treatments such different concentrations of Nitrite or others compounds (GNSO, NONOATE,…)
- RNA/DNA transfection
Department of Pathology (University of Pittsburgh)
01.2009 - 01.2011
- Tumor cell culture: Culture of human hepatoma cell lines (HepG2, HUH7, Hep3B, SNU449) and mouse liver cancer cell line (Hepa1-6); Also Hek293
- Western blotting, Immunohistochemistry, Tunel, Thymidine incorporation
- Intraperitoneal injections, tissue collection
- RNA/DNA transfections, Luciferase assay, DNA transformation
- Animal husbandry: Breeding, weaning, pup identification, replacement of breeders, create/maintaining new strains, monitoring special diets and genotyping
Education
Master of Science Degree - Pharmaceutics and Drug Design
University of Lille 2- Institute of Pharmaceutical Chemistry Albert Lespagnol
France01.2008
Bachelor’s Degree - Biochemistry and Molecular Biology
University of Sciences and Technologies of Lille 1
France01.2006
Skills
Techniques in Biochemistry & Immunology: Proficient in ELISA, ELISPOT, multi-color flow cytometry (FACS), and CD4 purification
Cell Culture & T Cell Expertise: Skilled in cell culture, immune cell isolation, activation, expansion, transfection, and transduction
Experimental Design & Data Analysis: Experienced in dose/response assays, data troubleshooting, and using GraphPad Prism, FlowJo, and LabChart View
Quality Control & Compliance: Knowledgeable in VEEVA systems and quality control protocols in research settings
Leadership & Communication: Strong organizational, mentoring, fluent in French and communication skills, with experience in team collaboration and presentations
Microsoft Office: Competent in Microsoft Office suite for documentation and research data presentation
Publications
- Mechanistic characterization of nitrite-mediated neuroprotection after experimental cardiac arrest., Cameron Dezfulian, Elizabeth Kenny, Andrew Lamade, Amalea Misse, Nicholas Krehel, Claudette St. Croix, Eric E. Kelley, Travis C. Jackson, Thomas Uray, Justin Rackley, Patrick M. Kochanek, Robert S.B. Clark, Hulya Bayir, J Neurochem., 11/01/16, 139, 3, 419–431
- P65: Nitrite after cardiac arrest has antioxidant effects reducing reperfusion cerebral hyperemia and subsequent caspase activation., Cameron Dezfulian, Amalea Misse, Jason P. Stezoski, Patrick M. Kochanek, Hulya Bayir, Robert S. Clark, Resuscitation, 05/01/14, 85, S93–S94
- Abstract 16029: Increased Brain Injury After Asphyxial Cardiac Arrest vs. Ventricular Fibrillation is Associated With Greater Brain Antioxidant Depletion., Andrew M Lamade, Thomas Uray, Elizabeth Kenny, Amalea Misse, Jason Stezoski, Tomas Drabek, Patrick Kochanek, Cameron Dezfulian, Circulation., 11/10/15, 132, Suppl 3x
Independentprojects
2011, Mouse model of iron-induced fibrosis and carcinogenesis- The effect of iron overload in the Beta-catenin liver knock out., TCPOBOP-induced cell proliferation is partially beta-catenin-dependent., Impact of Beta-Catenin inhibition on human hepatoma cells and mice; using beta-catenin antisense oligonucluotides.
Timeline
Senior Research Associate
PRECIGEN Inc
11.2018 - Current
Research Associate
Department of Neurology & Pharmacology (GWU)
01.2017 - 01.2018
Department of Critical Care Medicine- (The Safar Center)
01.2011 - 01.2017
Department of Pathology (University of Pittsburgh)
01.2009 - 01.2011
Master of Science Degree - Pharmaceutics and Drug Design
University of Lille 2- Institute of Pharmaceutical Chemistry Albert Lespagnol
Bachelor’s Degree - Biochemistry and Molecular Biology
University of Sciences and Technologies of Lille 1