Ashwani Kumar, Ph.D.
Galveston,TX
Summary
Highly motivated to work in the field of Structural biology and Protein chemistry using different tools and Techniques. Works diligently with teams to develop new technologies, improve processes and inspire innovation.
Overview
14
14
years of professional experience
Work History
Post Doctoral Fellow
UTMB Health
07.2020 - Current
- Solved the crystal structure of DFosB-JunD in a reduced state and in complex with a redox-switch-targeting compound at 1.94 and 3.21 Å respectively. DFosB belongs to AP1 transcription factor and has the ability to bind with other AP1 transcription factors e.g. JunD form Heterodimer. Published in Nucleic Acids Res (https:// doi .org/ 10.1093 / nar / gkac710).
- X-ray diffraction data of DFosB-JunD crystals were highly anisotropic and data were processed with different strategies to a resolution cutoff to get a better Electron density map.
- Expressed and Purified DFosB-JunD from Bacterial expression system and insect cells infected with recombinant baculovirus.
- Screening of pharmacological compounds interacting with DFosB or DFosB-JunD.
PhD (Graduate) Student
National Centre For Cell Science, Pune
02.2012 - 12.2019
- Solved the crystal structure of Rv0081 is a transcription factor that is transcription factor that is a central hub of the regulatory network of M. tuberculosis at 3.1 Å and characterized Rv0081 at molecular level using Biochemical and Biophysical techniques (CD, SEC-MALS, SPR).
- Wrote and published research article for Ph.D. project work. published in FEBS Letter https://doi.org/10.1002/1873-3468.13375
- During this tenure, had worked independently as well as in collaboration with other laboratories in India
Pre-doctoral Fellow; JRF
IIT Bombay
09.2009 - 07.2011
- Expressed and Purified Tau isoforms and Synucleins protein from tBacterial Expression system
- Purified tubulin from Goat brain.
- Interaction studies of tubulin and Tau isoforms using techniques Fluorescence (light scattering) CD, EM, and AFM
Education
Ph.D. - Biotechnology, Structural Biology
National Centre For Cell Science
Pune, Maharashtra, India 12.2019
Master of Science - Biotechnology
IIT Bombay
Mumbai, Maharashtra, India05.2009
Bachelor of Science - Chemistry
Banaras Hindi University
Varanasi, Uttar Pradesh, India08.2004
Skills
- Protein Expression (bacterial expression system, Mammalian, and Insect cells infected with recombinant baculovirus)
- Protein Purification (Affinity chromatography, Ion exchange, SEC)
- Protein Crystallization (vapour diffusion method- hanging, sitting drops)
- Structure determination (CCP4, Phenix, Coot)
- Biophysics (CD, Fluorescence)
- Biochemistry (SPR, SEC-MALS)
- EM (Negative staining EM grid Preparation, AFM grid Preparation)
Affiliations
- Ashwani Kumar, Galina Aglyamova, Yun Young Yim, Aaron O Bailey, Haley M Lynch, Reid T Powell, Nghi D Nguyen, Zachary Rosenthal, Wen-Ning Zhao, Yi Li, Jianping Chen, Shanghua Fan, Hubert Lee, William K Russell, Clifford Stephan, Alfred J Robison, Stephen J Haggarty, Eric J Nestler, Jia Zhou, Mischa Machius, Gabby Rudenko*:Chemically targeting the redox switch in AP1 transcription factor ΔFOSB (Nucleic Acids Res 2022 Sep 9;50(16):9548-9567. doi: 10.1093/nar/gkac710)
- Ashwani Kumar, Swastik Phulera, Arshad Rizvi, Parshuram J. Sonawane, Hemendra Singh Panwar, Sharmistha Banerjee, Arvind Sahu and Shekhar C. Mande*: Structural basis of hypoxic gene regulation by the Rv0081 transcription factor of M. tuberculosis (FEBS Letter Vol. 593, May 2019, Issue 9, Pages 982-995, https://doi.org/10.1002/1873-3468.13375)
- Ashwani Kumar, Swastik Phulera, Arshad Rizvi, Parshuram J. Sonawane, Hemendra Singh Panwar, Sharmistha Banerjee, Arvind Sahu and Shekhar C. Mande*: Deciphering the role hypoxia protein Rv0081 in latent M. tuberculosis. Journal of Proteins and Proteomics, Vol. 9, November 4, 2018, ISSN: 0975-8151
Work Availability
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Timeline
Post Doctoral Fellow
UTMB Health
07.2020 - Current
PhD (Graduate) Student
National Centre For Cell Science, Pune
02.2012 - 12.2019
Pre-doctoral Fellow; JRF
IIT Bombay
09.2009 - 07.2011
Ph.D. - Biotechnology, Structural Biology
National Centre For Cell Science
Master of Science - Biotechnology
IIT Bombay
Bachelor of Science - Chemistry
Banaras Hindi University