Chakri Srungarapu
Ellicott City
Summary
Biotechnology Scientist with expertise in isolating novel bioactive compounds and leading research teams. Isolated Streptomyces levis var. nov. SAROJA I8 from marine sediments, achieving 100% sequence similarity to known strains while exhibiting unique phenotypic traits. Developed Azaanthrocene-5,10-diol, demonstrating significant cytotoxicity against various cancer cell lines and antibacterial properties. Skilled in spectroscopic techniques and fermentation process optimization, dedicated to advancing impactful research initiatives.
Overview
10
10
years of professional experience
1
1
Certification
Work History
Scientist –Biotechnology
V.V Biotech Pvt Ltd
Bihar
10.2020 - 10.2021
- Led the isolation of microbial strains and studied soil aggregation through EPS production by Bacillus spp., contributing to soil health and biofilm research.
- Responsible for isolating and screening potential probiotic strains, optimizing culture media, and cultivating them for various applications.
- Utilized Solid State Fermentation (SSF) and Submerged Fermentation (SMF) techniques to study strains like Bacillus, E. coli, and S. aureus, optimizing growth and production conditions for specific bioactive compounds.
- Identified and isolated strains responsible for biofilm formation, utilizing the pour plate method.
- Conducted comprehensive biofilm assays and tested antibiotic susceptibility to evaluate bacterial resistance patterns.
- Analyzed infrared spectra and chemical composition of biofilm assays, ensuring accurate profiling of microbial interactions and metabolic products.
- Conducted bacterial adhesion assays on a wide range of strains, including Pseudomonas aeruginosa, S. aureus, S. epidermidis, E. faecalis, and actinomycetes, to understand microbial colonization and pathogenicity mechanisms.
- Skilled in analyzing experimental data, including DNA sequencing, PCR assays, and antibiotic susceptibility profiles.
- Prepared detailed reports and presentations for internal and external stakeholders.
- Adept at maintaining laboratory inventory, ensuring smooth operations, and providing guidance to junior scientists on experimental protocols, data analysis, and troubleshooting techniques.
- Evaluated potential safety risks, adhered to regulatory requirements, and maintained accurate records of all research activities in compliance with established standards.
Research Consultant
Jawaharlal Nehru Tropical Botanic Garden and Research Institute
Kerala
09.2018 - 01.2020
- Resourceful and results-driven Research Consultant with a strong background in experimental design, data analysis, and bioactive compound research.
- Collaborated with cross-functional teams to design and modify research experiments.
- Conducted in-depth research and analysis to generate valuable insights and reports.
- Proficient in isolating and characterizing bioactive compounds from marine sources, with expertise in optimizing fermentation parameters to enhance production.
- Skilled in utilizing advanced analytical techniques such as IR, H NMR, fermentation, and mass spectral analysis.
- Proven ability to evaluate compounds for their antimicrobial and cytotoxic activities.
- Actively contributed to the design and enhancement of curriculum as a member of the college curriculum committee.
- Analyzed experimental data, provided detailed reports, and supported supervisory teams in research project execution and documentation.
- Organized and maintained lab supplies, equipment, and inventories, ensuring smooth operation and compliance with safety standards.
- Developed and implemented innovative methods for collecting, analyzing, and interpreting data from surveys, interviews, and focus groups.
Asst. Professor
BAHIR DAR UNIVERSITY ETHIOPIA
BAHIR DAR
10.2017 - 08.2018
- Deliver engaging lectures and facilitate interactive discussions across diverse subjects such as Biochemistry, Molecular Biology, and Biotechnology.
- Mentor and guide Ph.D., MTech, and B. Tech students, supporting their academic and professional development.
- Organize and manage laboratory activities, including cell culture, insect cell expansion, and maintenance of lab inventories.
- Analyze and present experimental data, collaborate with supervisors, and assist in report writing and editing.
- Contribute to curriculum development and revisions as part of the academic committee.
- Foster international collaboration through MOUs with global institutions, enhancing educational and research opportunities.
- Organize seminars and workshops to share expertise and facilitate knowledge exchange.
- Provide career support for students, including internship and job search guidance.
- Maintain meticulous records, ensure compliance with lab protocols, and promote a positive learning environment.
Principal Investigator
WOMEN SCIENTIST SCHEME-A (WOS-A), under DST
Visakhapatnam
02.2012 - 02.2017
- Led the investigation of marine sediment samples from the Visakhapatnam coast (Bay of Bengal, India) to isolate actinomycetes to produce novel bioactive compounds, with a focus on antibacterial and cytotoxic activities.
- Successfully isolated and characterized a bioactive strain (Streptomyces levis var. nov SAROJA I8) using polyphasic taxonomic methods, including 16S rDNA sequencing (GenBank accession: KP890929).
- Optimized cultural and production conditions for the strain, leading to the isolation of Azaanthrocene-5,10-diol, a novel cytotoxic and antibacterial compound.
- Demonstrated potent anticancer activity (HeLa, MCF-7, U87MG) and antibacterial activity against both Gram-positive and Gram-negative bacteria.
- Authored the first report of Streptomyces levis isolated from a marine environment in India, contributing to the discovery of a new class of bioactive compounds with therapeutic potential.
- Developed and implemented research protocols for studies.
- Analyzed data to identify trends and patterns in research findings.
- Lead project teams of up to 10 researchers on multi-site projects.
- Provided direction and guidance to staff members regarding study design, data collection, analysis, and reporting of results.
- Managed the development of research databases and analysis tools.
Education
Ph.D. - Biotechnology
Andhra University
Visakhapatnam, Andhra Pradesh, India09.2017
M.Tech. - Biotechnology
Andhra University
Visakhapatnam, Andhra Pradesh, India01.2011
B.Tech. - Biotechnology
Al-Ameer College of Engineering and Information Tech
Visakhapatnam, Andhra Pradesh, India05.2008
Skills
- Isolation of novel bioactive compounds
- Proficiency in working with microorganisms
- Screening and analyzing microorganisms
- Conducting antibiotic susceptibility assays
- Downstream processing of macromolecules
- Development and execution of purification protocols
- Interaction with analytical and upstream functional areas
- Design and execute studies
- Standard operating procedures
- Data management
- Documentation and communication of results
- Sample and inventory management
- Optimization and development of fermentation processes
- Development of drug products
- Thin-layer chromatography
- Spectroscopic techniques
- Evaluating bioactive compounds
- Leading and mentoring research teams
- Organizing and prioritizing research assignments
- Quality control of suspensions
- Ensuring quality control
- Proper storage of microbiology samples
- Quality-related studies
- Preventative maintenance and calibration
- Production process monitoring
- Investigation of customer complaints
- Documentation for compliance
- Teaching various subjects in Biotechnology
- Mentoring students
- Strong communication skills
- Designing and planning research studies
- Analyzing research data
- Ensuring compliance with safety regulations
- Participation in training courses
Training
- Optimizing Fermentation Conditions for Producing Protease with Mangrove Actinomycete Isolate, BEST-2013 International Conference on Bioenergy, Environmental, and Sustainable Technologies, 01/27/13 - 01/30/13
- Production of Alkaline and Thermostable Polygalacturonase from a new strain of Bacillus subtilis C4, 100th Indian Science Congress, 01/03/13 - 01/07/13
Publications
Medium Optimization Studies Using Response Surface Methodology to Produce Cellulase from Banana Fruit Stalk Using Celluomonas uda NCIM 2353, 2010, The International Journal of Biological Science and Engineering
Patents
- 202241016808, A method of using hydrotropes in soap flotation for high recovery from low-grade ores, 03/24/22, 04/15/22
- 202241016805, A novel method for batch grinding laterite ore using statistical design, 03/24/22, 04/15/22
Accomplishments
Isolation of Streptomyces levis var. nov SAROJA I8 — 2012 – 2017
Isolated a new actinomycete strain from marine sediments, demonstrating distinct phenotypic characteristics and significant antibacterial and cytotoxic activities.
Affiliations
Training Periods:
· Fermentation and Bio Separation Methods Training Course Department of Microbiology, School of Life Sciences, Pondicherry University Duration: January 2nd, 2015, to January 17th, 2015, Sponsored by the Department of Biotechnology (DBT).
· Comprehensive Training in Pharmaceutical Processes AUROBINDO PHARMA LTD, Duration: November 27th to December 12th, 2006.
Certification
Patents:
· 202241016808, A method of using hydrotropes in soap flotation for high recovery from low-grade ores, 03/24/22, 04/15/22.
· 202241016805, A novel method for batch grinding laterite ore using statistical design, 03/24/22, 04/15/22.
References
References available upon request.
Timeline
Scientist –Biotechnology
V.V Biotech Pvt Ltd
10.2020 - 10.2021
Research Consultant
Jawaharlal Nehru Tropical Botanic Garden and Research Institute
09.2018 - 01.2020
Asst. Professor
BAHIR DAR UNIVERSITY ETHIOPIA
10.2017 - 08.2018
Principal Investigator
WOMEN SCIENTIST SCHEME-A (WOS-A), under DST
02.2012 - 02.2017
Ph.D. - Biotechnology
Andhra University
M.Tech. - Biotechnology
Andhra University
B.Tech. - Biotechnology
Al-Ameer College of Engineering and Information Tech
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