CHELSA SUSAN SUNNY

Proficient in conducting laboratory and field experiments while informing new hires on research methods and data collection procedures. Hardworking and driven with superior organizational and analytical skills, combined with unparalleled dedication to research excellence. Skilled in conceptualization, design, and implementation of microbiology, biochemistry, and molecular biology techniques to execute complex research projects in high-volume settings.

• Adelphi University, Garden City, NY, Graduate Student Researcher, 2024-01-01, 2025-05-01, Cloning of B-type response regulators in Physcomitrium patens, Investigate the role of cytokines, a class of plant hormones, in regulating plant growth and development, focusing on type-B transcription factors controlled by cytokine signaling., Utilize Physcomitrium patens as the model organism due to its genome sequencing and homologous recombination, aiming to clone the five type-B response regulators (RRB) for evolutionary signaling system analysis., Employ experimental techniques to isolate RNA from highly expressed tissues identified through the EFP browser, followed by cDNA synthesis and amplification using reverse transcriptase PCR, and subsequent cloning of transcription factors into the gateway system for further analysis., Utilized bioinformatics tools for sequence analysis, gene expression studies, and evolutionary pathway mapping., Conducted fluorescence microscopy for cellular and molecular imaging, including detection of gene expression markers., Performed RT-PCR for gene expression analysis, optimizing reaction conditions for accurate quantification.
• Mahatma Gandhi University, India, Student Researcher, 2023-04-01, 2023-04-30, PanGenome based approach to predict the AMR genes and virulence genes in E. coli, Conducted whole genome sequencing and comparative genome analysis with strains isolated from animals and humans., Performed PanGenome analysis, identification of AMR genes, detection of virulent genes, plasma gene analysis and phylogenetic tree construction based on the data., Used software tools ROARY, ABRicate, VFDB, Plasmid finder, MEGA.
• PGM College MG University, India, Researcher, 2017-12-01, 2017-12-31, Isolation of Biofilm-Producing Bacteria from Toothbrush, Followed a systematic protocol to sample toothbrush bristles or rinse them with a sterile solution to collect surface bacteria., Transferred collected samples onto suitable growth media for bacterial culture and ensured optimal growth conditions by incubating agar plates at 37C (98.6F) for 24 - 48 hours., Identified potential biofilm-producing bacteria colonies based on characteristics observed post-incubation.

• Cloning and characterization of B-type transcription factors in Physcometrium patens to unveil the evolutionary dynamics of signalling pathways, 2024-04-01, 21st AU Scholarship and Creative Works Conference
• Cloning and characterization of B-type transcription factors in Physcometrium patens to unveil the evolutionary dynamics of signalling pathways, 2025-04-01, 22nd AU Scholarship and Creative Works Conference