D'Andre Conaway

• Support the Upstream Process Development team within Manufacturing Sciences, Analytics, and Technology with continuous improvment of the licensed influenza vaccine (Flublok) and a novel SAR-CoV-2 vaccine (VidPrevtyn)
• Maintenance of insect cells in suspension culture at different scales
• Transformation, Midi, and Mini prep for plasmid DNA purification
• Purification of Extracellular Viral DNA for co-transfection
• Co-transfection of insect cells, baculovirus plaque purification, infection & scale-up of baculovirus stocks
• Sampling/harvesting cell culture for preparation of samples for analytical testing and the following viral passage
• Perform molecular biology tasks such as baculovirus DNA purification, PCR, restriction digestion, DNA agarose gel electrophoresis
• Phenol/chloroform extraction of HMW DNA from viral supernatant
• Utilize Agilent Femto Pulse for HMW DNA analysis
• Performing analysis of recombinant protein expression by Western blot, SDS-PAGE, and single radial immunodiffusion techniques (SRID)
• Responsible for documentation of each task
• Maintenance of the labs and ordering of supplies
• Communicate with co-workers and present data on a regular basis during department meetings
• Accomplish all tasks within established timeframes
• Maintained professional, organized, and safe environment for employees and patrons.
• Cross-trained existing employees to maximize team agility and performance.
• Enhanced research quality by optimizing molecular biology protocols, such as PCR and DNA extraction.
• Maintained detailed records of experimental procedures and results, ensuring transparency and reproducibility in molecular biology techniques

NGS library preparation:

• Poly (A) RNA seq library construction
• Whole Genome Sequencing library construction
• In situ Chromatin Interaction Analysis Using Paired-End Tag Sequencing (ChIA-PET) - ENCODE Project
  - Cell lysis & nuclear permeabilization
  - In situ restriction digestion
  - A-tailing & Proximity ligation
  - Chromatin Immunoprecipitation (ChIP)
  - Transposase driven fragmentation (tagmentation)
  - Illumina library PCR
  - DNA Size selection AMPure XP Dual-sided, BluePippin
  

Quality/quantity evaluation:

• DNA/RNA purification
• RT-qPCR
• Qubit DNA/RNA quantification
• Agilent 2100 DNA/RNA size and quality assessment

• Handled up to 11, 000 SARS-CoV-2 specimens daily
• Utilize robotic systems for Ultra-high throughput SARS-CoV-2 extraction and PCR testing
• Process patient specimens according to priority while maintaining an overall turnaround time of approximately 24 hours or less
• Re-array 384-well specimen source plates to discard true negative samples and consolidate true positive samples for further research
• Maintain confidentiality of all patient information to remain compliant with HIPAA
• Keep close contact with A&R to ensure that failed or inconclusive samples are reworked appropriately
• Manually re-extract and repeat PCR for failed or inconclusive samples
• Perform experimental processes to facilitate the launch of JAX's PCR testing program for detection of SARS-CoV-2 in mice
• Performed SARS-CoV-2 limit of detection (LoD) experiments using hACE2 mice
• Maintain a clean laboratory environment to prevent contamination
• Keep equipment in good working order for optimal functionality and longevity

• Receive, triage, and accession SARS-CoV-2 samples in accordance with applicable SOPs
• Utilize Clarity LIMS to track samples and ensure fluid transitions through each step of the process
• Review patient documentation and file accordingly
• Evaluate patient specimens for adequacy and acceptability
• Re-stock PPE and other necessary equipment
• Prepare virucidal agents for laboratory decontamination

• Communicated with patients to collect information about prescriptions and medical conditions or arrange consultations with pharmacists
• Consulted with insurance company representatives to complete claims processing, resolve concerns and reconcile payments
• Stocked, labeled and inventoried medication to keep accurate records.
• Restocked pharmacy shelves with current merchandise to drive consistent peripheral sales
• Counted, measured and reconstituted medications following standard procedures
• Assisted pharmacist by filling prescriptions for numerous customers daily and responding to patient questions regarding prescriptions
• Answered incoming phone calls and addressed questions from customers and healthcare providers

• Processed paperwork such as patient discharge orders and transportation orders
• Reviewed patient records, paperwork and physician orders
• Interacted with all necessary partners throughout the department, including physicians, nursing staff, technicians and medical assistants.
• Completed patient charts prior to discharge, thus to maintaining an efficient and organized workflow
• Complied with HIPAA rules and regulations regarding patient confidentiality.