DAPHNE THERESE ALBERT
Germantown,MD
Summary
Detail-oriented team player with strong organizational skills and the ability to handle multiple projects simultaneously with a high degree of accuracy. Sharp and focused professional offering skills in Immunology and cell biology.
Overview
3
3
years of professional experience
1
1
Certification
Work History
Scientist
B-Cell Technologies
01.2023 - Current
- Establish novel immunization strategies for complex membrane protein targets
- Generate monoclonal antibodies against different therapeutic targets and optimize High Through Put (HTP) cell-based screening platforms for antibody discovery.
- Performed complex troubleshooting and assisted in leading initiatives to solve challenging scientific problems with assays or instrumentation.
Co-op
Cell Assay, EMD Serono
01.2022 - 06.2022
- Assisted in antibody hit generation, screening, and target binding
- Harvest tissues and isolate lymphocytes from murine specimens and perform in-vitro cell functional assays
- Large scale mammalian cell and tissue culture and cryopreservation, developing and performing biochemical and immunological assays such as FACS, and ELISA
- Trained in PCR techniques, electrophoresis gel runs, and B-cell sorting using flow cytometer.
Document Assistant
UMass Lowell
09.2021 - 12.2021
Starbucks barista
UMass Lowell
01.2021 - 09.2021
Education
Master of Science - Biomedical Engineering and Biotechnology
University of Massachusetts Lowell
Lowell, MA12.2022
Bachelor of Technology - Biotechnology
DY Patil School of Biotechnology And Bioinformatics
Navi Mumbai, India07.2019
Skills
- Laboratory Skills
- Proficient in procedures of ELISA, FACS, hybridoma fusion & screening, limited dilution control (LDC) and single-cell sorting to obtain monoclonal populations, murine tissue collection, PBMC isolation, cell culture, cryopreservation, BSL2 handling, DNA isolation, PCR, Gel Electrophoresis, SDS-PAGE, Western Blotting, recombinant protein expression techniques involving cell transfection, selection and purification via chromatography and dialysis
- Handling of UV spectrophotometer, flame photometer and colorimeter
- Software, Technical and Data Analytical Skills
- Proficient with NCBI, EMBL databases and programs ie: Swiss-Model, Genescan, TCoffee, Clustal Omega, dbSNP, Ensembl
- Proficient with software ie: Prism Graphpad, GeneData, RasMol, SPDBV, Arguslab, ChemSketch, HyperChem, Hex protein docking, and IGemDock
Accomplishments
- B
- Tech Dissertation Nov 2018 – May 2019
- Location: Indian Institute of Technology, Powai, Bombay – Dept
- Of Biosciences and Bioengineering
- Title: ‘Characterization of Globular Glial Endopathy Causing K317N Mutation of 4R2N Tau'
- Objective: To incorporate a single mutation K317N into the full-length tau protein by PCR-based site-directed mutagenesis on its respective MAPT gene in order to study the molecular mechanism of its pathogenicity
- Mentor: Dr
- Dulal Panda, Chair Professor
- Individual Role- To isolate and incorporate a single mutation (K317N) in the DNA via PCR-based site-directed mutagenesis
- Induce protein expression in transformed cells followed by protein isolation and concentration
- Biochemical assays-thioflavin S
- Fluorescence and tubulin polymerization to study the aggregation propensity of the single mutant with respect to the wild type
- Bioinformatics Mini Project Jan 2018 – Feb 2018
- Location: D.Y
- Patil School of Biotechnology and Bioinformatics, India
- Title- Diabetes in Pregnancy: Gestational Diabetes
- Website Link-https://diabetes-in-pregnancy.000webhostapp.com/
- Objective: To raise awareness among young mothers and providing the viewers with diet plans and the precautionary measures they are to take during their pregnancy
- Individual Role- To research well on the cause, effect and treatment related to gestational diabetes and create a self-explanatory website.
Certification
• Instrumentation training | Varni labs, Mumbai Dec 2017
• Immunology and Virology workshop | Haffkines Institute, Mumbai June 2017
• Quantitative Methods for Biology – MATLAB | Harvard University(EdX) Nov 2020
• The Science of Stem Cells | American Museum of Natural History(Coursera) Nov 2020
• Human Subjects Research Training | CITI program Sept 2021
EXTRACURRICULAR ACTIVITIES AND INTERESTS
• Participated in college level and inter-school debate competitions.
• Active member of the church choir and a Sunday school catechism teacher
• Enjoy reading mystery or thriller-based novels.
• Learning to play the ukulele.
Languages
English
Full Professional
Hindi
Native or Bilingual
Tamil
Native or Bilingual
Timeline
Scientist
B-Cell Technologies
01.2023 - Current
Co-op
Cell Assay, EMD Serono
01.2022 - 06.2022
Document Assistant
UMass Lowell
09.2021 - 12.2021
Starbucks barista
UMass Lowell
01.2021 - 09.2021
Master of Science - Biomedical Engineering and Biotechnology
University of Massachusetts Lowell
Bachelor of Technology - Biotechnology
DY Patil School of Biotechnology And Bioinformatics
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