Debojyoti Lahiri

Strong leader and problem-solver dedicated to streamlining operations to decrease costs and promote organizational efficiency. Uses independent decision-making skills and sound judgment to positively impact company success. 12 years of post-Ph.D. oncology and molecular biology experience in designing and performing cell based bioassays, monoclonal antibody discovery and development, with hands on experience on leading students and team of scientists on different projects involving protein quantitation and purification, genetic screens, electrophoretic and column chromatographic techniques in protein analysis, biochemical techniques such as qPCR, HPLC, DNA sequencing, SDS-PAGE, CE, Densitometry and various immunoassays such as ELISA, IP, IF etc. in cGMP. Experienced with inter-organizational collaboration, investigations and authoring standard operating procedures (SOP). Well-organized, Motivated, and hardworking individual with good communication skills and efficient in deviation processes.

Knowledge

· Good Laboratory Practices (GLPs), Good Documentation Practices (GDPs), Good Manufacturing Practices (GMPs)

· Validation protocols and reports, analytical test procedures, technical reports, and change controls Equipment Validation

· Cell Culture, Aseptic Technique

· Potentiometry,

· Assay Data Review

· Method qualification/validation

· Karl Fischer Titration

· GC/MS, LC/MS

· Knowledge of FDA, EPA, ICH requirements

· Read, Edit and Create Standard Operating Procedures (SOPs)

· Light Scattering, Micro-Flow Imaging

· Capillary Electrophoresis

Methods/Instrumentation

∙ ELISA/ EIA, BioTek Eon Plate Reader

∙ Fluorometric Spectroscopy

∙ DNA, RNA and Protein purification

∙ SEC-MALLS

∙ R, Origin Pro, Graph Pad Prism

∙ Protein purification

∙ Medicinal chemistry, biochemistry,Molecular biology and genetics

∙ Capillary electrophoresis Beckman Coulter System

∙ Ligand Binding Assay

∙ qPCR, RT PCR (StepOne)

∙ Waters HPLC

∙ Cellular imaging

∙ BD flow cytometer

∙ CCD X-ray diffractometer

∙ Maldi-TOF MS

∙ Immunoassays MTT, Immunoblotting, IF, IP

∙ FTIR, UV Spectroscopy

∙ NMR

∙ Cell Based Bioassay

∙ Fermenters/Bioreactors

∙ Technical reports and scientific journal publications and presentation

∙ Wet chemistry and molecular synthesis

∙ Capillary electrophoresis Beckman Coulter System

∙ DNA binding, DNA melting, viscometry, cyclic voltammetry,

Skills

∙Analysis of leachable and extractable materials from Silicone tubing and bagging ∙Wet chemistry and molecular synthesis ∙Osmolality ∙Bioconjugation chemistry ∙Ligand Binding assay ∙Scientific writing and publishing in top international peer reviewed journals ∙X-ray Crystallography ∙FT-IR ∙UV-Visible ∙Fluorescence ∙EPR ∙NMR ∙ESI ∙MALDI-TOF MS spectroscopy ∙Magnetism ∙Electrochemistry ∙Molecular modeling ∙Theoretical calculation of energy minimization ∙HPLC (reverse phase, IEC, SEC) ∙Empower 3 ∙Capillary electrophoresis ∙DNA, and RNA purification ∙Mammalian cell culture and probiotics such as Yeast ∙in vitrocytotoxicity ∙Microscopy and Cellular imaging ∙MTT assay ∙ELISA ∙MSD-ECL immunoassay ∙LASER assisted DNA cleavage ∙DNA binding ∙DNA melting ∙Viscosity studies ∙pH meter ∙Recombinant protein/mAb analysis purification and storage ∙Immunoprecipitation ∙Immunofluorescence ∙Immunoblotting ∙Agarose gel electrophoresis and SDS PAGE ∙Flow Cytometry ∙FACS ∙Monoclonal antibody purification and engineering ∙DNA manipulation and site directed mutagenesis ∙DNA sequence analysis ∙PCR amplification ∙cloning ∙RT-PCRand qPCR ∙ Antibody Development and purification ∙Circular Dichroism, ∙Fluorescence ∙Differential Scanning Calorimetry ∙ Image J and Graph Pad Prism

Awards, Affiliations and Hobbies

● 2019-2021 – 4 “Inspire” awards, 4 “Impact” awards and one “Living Our Values” and one “Superlative Service” award from Eurofins.

● 2010 - Awarded Post-doctoral fellowship from Department of Biotechnology (India).

● 2006 - Awarded Senior Research Fellowship (SRF) by Indian Institute of Science, Bangalore, India.

● 2004 - Awarded Junior Research Fellowship (JRF) by Indian Institute of Science, Bangalore, India.

● 2004 - Qualified in National Eligibility Test (NET) (CSIR), India for pursuing lecturership at the national level.

● 2004 - Qualified in the Graduate Aptitude Test in Engineering (GATE), for pursuing doctoral degree at the national level.

● Scientific reading and writing, science communication.

● Published photographer in National Geographic Society, BBC, and Discovery, Lonely planet and Outdoor Photographer.