Expert translational immunologist in immunomodulatory mechanisms in atopic disorders, Co-inventor on 1 approved US Patent), vaccine development (Co-inventor on 2 filed pending patents), lupus disease and B lymphocyte development. Flexible, versatile & an independent researcher with handling de-identified human blood samples, PBMC preparation for immunophenotyping of immune cells such as NK cells and T-cells using flow cytometry and perform ex-vivo functional assays and isolate immune cells from non-human primate tissue (lymph node & blood) and perform in-vivo mouse inflammatory models. Ability to work cross-departmentally, fostering internal & external collaborations with institutions & corporate sectors and present scientific data at internal and international conferences as evidenced by international conferences presentation and travel awards.
Work History
Research Associate
Cincinnati Children’s Hospital Center
Ability to quickly assess complex situations, design, plan and execute mouse models for vaccine models, lupus disease and CAR-NK cells (cellular therapy), B-lymphocyte development as evidenced by abstracts and presentations in conferences
Ability to troubleshoot and resolve technical issues and use immunological methods such as isolation of primary immune cells such as NK cells from human blood samples and feeder-cell and feeder cell free based NK cell expansion
Proficient in isolation of NK cells from non-human primate PBMC and lymph node and expand using feeder cell system for proof-of-concept CAR NK cells, cryopreservation of expanded NK cells, transfection using Maxcyte system
Ability to write clinical regulatory documents and contribute to IACUC protocols & IBC amendments, material transfer agreements with academic institutions for research reagents & resources, and non-profit organizations while mentoring students.
Postdoctoral Fellow
Cincinnati Children’s Hospital Center
Expertise in implementing effective plans for solutions, and work with mouse models of allergic diseases such as food allergy, airway hyperresponsiveness through skin, intranasal, intraperitoneal injections leading to extra-mural grants from NIH (National Institute of Health) and DoD (Department of Defense)
Expert co-inventor, strategic planner, high-level technical, engaged in exploratory & in drug discovery projects and perform desensitization experiments in mouse models with established food allergy and compare innovative strategy to target IgE receptor, FceRIa using antibody on mast cells & other effector cells such as basophils and eosinophils leading to 1 approved US Patent # 9868786 (in commercialization phase, Fresno Therapeutics)
Achieved [Result] by completing [Task] with accuracy and efficiency.
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Achieved [Result] through effectively helping with [Task].
Grants
Department of Defense (DoD): PR120718 (Fred D Finkelman (P.I.) & Durga Krishnamurthy (Co-P.I), Project Title: Induction of food allergy in mice by allergen inhalation.
Intermountain Medical Center (University of Cincinnati) Fred D Finkelman (P.I.) & Durga Krishnamurthy (Co-P.I.), Project Title: Rapid Suppression of Food Allergy with AntiFcεRIa antibody.
Methods For Suppressing Allergic Reactions, Finkelman, Fred; Khodoun, Marat, V.; Kucuk, Zeynep, Yesim; Krishnamurthy, Durga; Strait, Richard, T. Patent No: O/2012/142286; International application No. PCT/US2012/033319; Approved on Jan 2018 (in commercialization phase, Fresno Therapeutics)
Pending Patents
Co-inventor on 2 pending patents, innovative vaccine strategies
Publications Abstracts
David E Ochayon, Stanley B DeVore, Wan-Chi Chang, Durga Krishnamurthy, Harsha Seelamneni, Brittany Grashel, Daniel Spagna, Sandra Andorf, Lisa J Martin, Jocelyn M Biagini, Stephen N Waggoner, Gurjit K Khurana Hershey. Progressive accumulation of hyperinflammatory NKG2Dlow NK cells in early childhood severe atopic dermatitis. Science Immunology, Vol 9, Issue 92, 2024.
Durga Krishnamurthy, Harsha Seelamneni, David Ochayon, John B. Harley and Stephen N. Waggoner, Conditional abrogation of Ddx3x reveals its essential role in lymphocyte development and partial compensation by Y chromosome, J Immunol May 1, 2020, 204 (1 Supplement) 223.19 (AAI Trainee Abstract Award 2020) (Manuscript in revision)
Durga Krishnamurthy, Hilal Cevik, Ayad Ali, Laura Canaday, Eshana Mukhopadhyay, William Seibel, Kimberly Risma, Michael B Jordan, Stephen N Waggoner. Inhibition of perforin enhances T cell and humoral responses after immunization. J Immunol (2022) 208 (1_Supplement): 123.04. (US Patent filed December 2020 and manuscript in preparation)
Yuting Tang, Qian Xu, Liang Hu, Xiaomei Yan, Xiaomin Feng, Asumi Yokota, Weinan Wang, Di Zhan, Durga Krishnamurthy, David E Ochayon, Lijun Wen, Li Huo, Huimin Zeng, Yingwan Luo, L Frank Huang, Mark Wunderlich, Jiwang Zhang, Eric Vivier, Jianfeng Zhou, Stephen N Waggoner, Gang Huang. Tumor Microenvironment–Derived R-spondins Enhance Antitumor Immunity to Suppress Tumor Growth and Sensitize for Immune Checkpoint Blockade Therapy. Cancer Discovery (2021) 11 (12): 3142–3157. (Isolation of tumor infiltrating immune cells & cytotoxicity assay)
Kelly M Rangel, Seth D Reighard, David E Ochayon, Durga Krishnamurthy, Wen-Hai Shao and Stephen N Waggoner Cell therapy targeting follicular T cells as an innovative approach for treating autoimmune disease, J Immunol May 1, 2020, 204 (1 Supplement) 236.9). (CAR-T cells & CAR-NK cells).
Seth D Reighard, Durga Krishnamurthy, Hilal Cevik, David E Ochayon, Ayad Ali, Harsha Seelamneni, Hermine I Brunner, Stephen N Waggoner. Immunomodulatory effects of cytokine-induced expansion of cytotoxic lymphocytes in a mouse model of lupus-like disease. Cytotherapy, Volume 23, Issue 1, Jan 2021, 37-45. (Cellular therapy).
2 AAI Travel awards, OEGAI member, Austrian Society for Allergology and Immunology, AAI Member, American Association of Immunologists, Travel, Philanthropy, Poetry, Cooking & Baking International Cuisines
Coordinator – Program Manager at Children’s for Children Early Education and Care, Cincinnati Children’s Hospital Medical CenterCoordinator – Program Manager at Children’s for Children Early Education and Care, Cincinnati Children’s Hospital Medical Center
Assistant Vice President of Operations at Cincinnati Children’s Hospital Medical CenterAssistant Vice President of Operations at Cincinnati Children’s Hospital Medical Center