Edmund Carvalho

Stem cell and mitochondrial researcher with 9+ years of experience in CRISPR/Cas9 gene editing, metabolic profiling, and nanoparticle-based drug delivery. Demonstrated expertise in translational immunometabolism, cellular imaging, and iPSC differentiation, with multiple high-impact publications and a postdoctoral funded fellowship from AHA.

• Team member, ABLE BEST-DBT biotechnology-based business plan competition-2009, India (In top 5 teams out of 130+ entries)
• Awarded NCL scholarship, Department of Biochemistry, Pune University, 2004-2006

• American association of Immunology (AAI)
• American society for cell biology (ASCB)
• American heart association (AHA)

• Immunometabolism
• Mitochondrial biology
• CRISPR knockout screens
• CRISPR/Cas9 knockin/knockout models
• Nanomedicine
• Stem cell biology
• Novel Drug Delivery Systems
• Controlled drug release
• In situ gelling biomaterials
• Primitive and definitive hematopoiesis

• Immunometabolism: T cell biology, mitochondrial dynamics in T cells, Mitochondrial calcium dynamics (Fluo4/Rhod2), Imaging on Celigo HT platform.
• Flow cytometry: BD Fortessa, BD Symphony, BD Facs Melody.
• Mitochondrial Energetics: Agilent-Seahorse (XFe96), Oroboros high resolution respirometer-ATP stress test.
• Gene expression: gBLOCK preparation, verification of transcript length, purity and suitability through gel electrophoresis.
• Yeast transformation and expression: yeast preparation of competent cells, transformation and expression of Mito plasmids.
• Western blot and Immuno precipitation (IP): Protein quantification(BCA, Nanodrop), Gel electrophoresis followed by membrane transfer.
• FPLC: FLAG and HA tagged protein expression, stress treatment, protein extraction.
• HPLC: Drug solubilization from media and tissue, and estimation.
• Calcium flux measurements: Spectrophotometric analysis of mitochondrial calcium retention capacity.
• Imaging(Confocal): Sample preparation and imaging on Electron microscopy.
• Genome-wide CRISPR Knockout Library: Pooled library transfection, stress screen, colony screening and isolation.
• CRISPR/Cas9 knockin/knockout: gRNA design for Cas9 and Cas9n strategies.
• Animal experience: Breeding for Knock-out generation, animal maintenance, Surgery in mouse model.
• Pluripotent stem cell growth and culture: Growth and maintenance of embryonic and induced pluripotent stem cells.
• 3-D differentiation of pluripotent stem cells: Optimization of protocols differentiation of pluripotent stem cells.
• Nanoparticle preparation and characterization: Lipid and polymer nanoparticle preparation.
• Hydrogel preparation and characterization: Preparation and characterization of in situ gelling hydrogels.
• Mouse embryonic fibroblast: Isolation of day 12-14 mouse embryos post coitum.
• Ex Situ models: Development of Ex Situ models for hydrogel testing.
• Microbiology: Screening of microbes, Handling and preservation of microorganisms.

• Nanoparticulate in-situ gels of TPGS, gellan and PVA as vitreous humor substitutes., US 8343471, Edmund Carvalho, Rinti Banerjee
• Nanoparticulate in-situ gels as vitreous humor substitutes for ocular diseases., US 20120082730, Edmund Carvalho, Rinti Banerjee

• Definitive differentiation recapitulates fetal globin levels with a potential for generating clinically relevant RBC populations., Gordan Red Cell Conference 2025, Poster
• Mitochondria Cristae disturbance promotes optimal T cell activation., Cellicon 2023
• Mitochondria Cristae disturbance promotes optimal T cell activation., American Association of Immunology 2023, Washington DC, Poster
• MIRO-1 Determines Mitochondrial Shape Transition upon GPCR Activation and Ca2+ Stress., ASCB 2018, San Diego
• Development of Dual Triggered In-situ Gelling Scaffolds for Tissue Engineering., BIT’s 5th Annual World Gene Convention-2014, Qingdao, China, 2015-11-13 to 2015-11-15, Invited talk
• Nanoparticles for cardiac regeneration., 8th BITS conference on regenerative medicine and stem cells, Shanghai, China, 2015-11-18 to 2015-11-20, Invited talk
• Tunable hydrogels for differentiation of tissue progenitors using nanoparticles., 40th Annual Meeting & Exposition of the Controlled Release Society, 2013-01-01
• Development of dual triggered in-situ gelling scaffolds for tissue engineering., 7th International Conference on Materials for Advanced Technologies, 2013-01-01
• Development of scaffolds for cardiac differentiation of pluripotent stem cells., World Congress on Reproductive Biology, Cairns, Australia, 2011-01-01
• IMARIS training workshop, University of Delaware, 2019-12-01, Delaware, USA
• COE workshop on Biomolecular interactions., 2015-11-01, NCBS Bangalore, India
• Practical training modules in animal handling., 2011-02-01, Monash University Australia
• Paper writing., 2011-03-01, Monash University Australia
• Entrepreneurship workshop., 2009-01-01, ABLE-BEST INDIA

• Abstract and poster review, 2024-2025
• Abstract review, CHOP travel grant, 2024-2025

• Demonstrator, BCH 3031: Advanced Molecular Biology: Modern concepts and applications, Handling practical sessions, assessments and teaching concepts for a group of 10 students., Department of Biochemistry, Monash University, 2012-01-01 to 2012-07-01
• Teaching assistantship, IIT Bombay, 2009-01-01 to 2015-01-01
• Biosafety officer, Nanomedicine lab, IIT Powai, Mumbai, 2009-01-01 to 2014-01-01
• Core group, IITB-Monash student leadership, 2015-01-01 to 2016-01-01
• General Secretary of Hostel-12, Housing 500 students, 2007-01-01 to 2008-01-01

• Title: Research Associate Scientist II
• Marital Status: Married
• Residence Permit: Permanent Residency (Green card holder)
• Visa Status: Permanent Residency (Green card holder)

• Team member, ABLE BEST-DBT biotechnology-based business plan competition, 2009, India, In top 5 teams out of 130+ entries
• Awarded NCL scholarship, 2004-2006, Department of Biochemistry, Pune University

• Riley J, Deng M, Lai M, Jiang S, Kong H, Cooper V, Carvalho E, Gu M, Nardo D, Zhang C, Hanindya C., Cell-derived nanoparticles generated from cell-based artificial antigen presenting cells allow for the robust and rapid manufacturing of patient T cells., Cytotherapy, 2025-05-01, 27, 5, S199-200
• Gu M, Carvalho EJ, Read KA, Nardo DP, Riley JL., Rab5 Overcomes CAR T Cell Dysfunction Induced by Tumor-Mediated CAR Capture., bioRxiv, 2024-07-26, 10.1101/2024.07.26.605334
• Carvalho EJ, Stathopulos PB, Madesh M., Regulation of Ca2+ exchanges and signaling in mitochondria., Curr Opin Physiol, 2020-10-01, 17, 197-206
• Tomar D, Jaña F, Dong Z, Quinn WJ 3rd, Jadiya P, Breves SL, Daw CC, Srikantan S, Shanmughapriya S, Nemani N, Carvalho E, Tripathi A, Worth AM, Zhang X, Razmpour R, Seelam A, Rhode S, Mehta AV, Murray M, Slade D, Ramirez SH, Mishra P, Gerhard GS, Caplan J, Norton L, Sharma K, Rajan S, Balciunas D, Wijesinghe DS, Ahima RS, Baur JA, Madesh M., Blockade of MCU- Mediated Ca2+ Uptake Perturbs Lipid Metabolism via PP4-Dependent AMPK Dephosphorylation., Cell Rep, 2019-03-26, 26, 13, 3709-3725.e7
• Sarkar A, Carvalho E, D'souza AA, Banerjee R., Liposome-encapsulated fish oil protein-tagged gold nanoparticles for intra-articular therapy in osteoarthritis., Nanomedicine (Lond), 2019-04-01, 14, 7, 871-887
• Woods JJ, Nemani N, Shanmughapriya S, Kumar A, Zhang M, Nathan SR, Thomas M, Carvalho E Ramachandran K, Srikantan S, Stathopulos PB, Wilson JJ, Madesh M., A Selective and Cell-Permeable Mitochondrial Calcium Uniporter (MCU) Inhibitor Preserves Mitochondrial Bioenergetics after Hypoxia/Reoxygenation Injury., ACS Cent Sci, 2019-01-23, 5, 1, 153-166
• Nemani N
• Carvalho E
• Tomar D, Dong Z, Ketschek A, Breves SL, Jaña F, Worth AM, Heffler J, Palaniappan P, Tripathi A, Subbiah R, Riitano MF, Seelam A, Manfred T, Itoh K, Meng S, Sesaki H, Craigen WJ, Rajan S, Shanmughapriya S, Caplan J, Prosser BL, Gill DL, Stathopulos PB, Gallo G, Chan DC, Mishra P, Madesh M., MIRO-1 Determines Mitochondrial Shape Transition upon GPCR Activation and Ca2+ Stress., Cell Rep, 2018-04-24, 23, 4, 1005-1019
• Dong Z, Shanmughapriya S, Tomar D, Siddiqui N, Lynch S, Nemani N, Breves SL, Zhang X, Tripathi A, Palaniappan P, Riitano MF, Worth AM, Seelam A, Carvalho E, Subbiah R, Jaña F, Soboloff J, Peng Y, Cheung JY, Joseph SK, Caplan J, Rajan S, Stathopulos PB, Madesh M., Mitochondrial Ca2+ Uniporter Is a Mitochondrial Luminal Redox Sensor that Augments MCU Channel Activity., Mol Cell, 2017-03-16, 65, 6, 1014-1028.e7
• Lee SK, Shanmughapriya S, Mok MCY, Dong Z, Tomar D, Carvalho E, Rajan S, Junop MS, Madesh M, Stathopulos PB., Structural Insights into Mitochondrial Calcium Uniporter Regulation by Divalent Cations., Cell Chem Biol, 2016-09-22, 23, 9, 1157-1169
• Sehgal RR, Carvalho E, Banerjee R., Mechanically Stiff, Zinc Cross-Linked Nanocomposite Scaffolds with Improved Osteostimulation and Antibacterial Properties., ACS Appl Mater Interfaces, 2016-06-08, 8, 22, 13735-47
• E Carvalho, P Verma, K Hourigan, R Banerjee., Myocardial infarction: stem cell transplantation for cardiac regeneration., Regenerative medicine, 2015-01-01, 10, 8, 1025-1043
• Singh PK, Ghosh D, Tewari D, Mohite GM, Carvalho E, Jha NN, et al., Cytotoxic Helix-Rich Oligomer Formation by Melittin and Pancreatic Polypeptide., PLoS ONE, 2015-01-01, 10, 1, e0120346
• Ghosh D, Sahay S, Ranjan P, Salot S, Mohite GM, Singh PK, Dwivedi S, Carvalho E, Banerjee R, Kumar A et al., The newly Discovered Parkinson's Disease Associated Finnish Mutation (A53E) Attenuates α- Synuclein Aggregation and Membrane Binding., Biochemistry, 2014-01-01, 53, 41, 6419-6421
• Carvalho E, Verma P, Hourigan K, Banerjee R., Development of dual-triggered in situ gelling scaffolds for tissue engineering., Polymer International, 2014-01-01, 63, 1593-1599
• Jain D, Carvalho E, Banthia A and Banerjee R., Development of polyvinyl alcohol–gelatin membranes for antibiotic delivery in the eye., Drug Development and Industrial Pharmacy, 2011-01-01, 37, 167-177
• Jain D, Carvalho E and Banerjee R., Biodegradable hybrid polymeric membranes for ocular drug delivery., Acta Biomaterialia, 2010-01-01, 6, 1370-1379

was Awarded theAHA post-doctoral fellowship for "Genome-wide CRISPR screen identifies S100Z as a key molecular linker for calcium overload induced cell death"