Summary
Overview
Work History
Education
Skills
Awards
Publications
Timeline
Generic

Funien Tsai

Pleasanton,CA

Summary

With over a decade of expertise in genomics research and data analysis, I am a dedicated professional leveraging a data-centric approach to tackle challenges in cell and molecular biology. I bring more than 3.5 years of experience in Product Development Projects centered on single-cell genomics, leading initiatives and contributing to published research findings.

Overview

6
6
years of professional experience

Work History

Scientist

10x Genomics
Pleasanton, CA
07.2020 - Current
  • Led assay optimization using new reverse-transcription chemistry resulting twofold increase in sensitivity
  • This breakthrough enhances our ability in analyzing genomic profiles of varies cell types which broadens therapeutic applications
  • Supported the successful launch of the 5'CRISPR product and presented key results at the 5'CRISPR launch webinar in 2022, reaching an audience of ~130 industry professionals
  • Conducted data analysis for a combinatorial drug screening at a scale over 500k single-cells which is authored a published data spotlight, highlighting the successful utilization of a high-throughput assay product, showcasing analytical expertise
  • Mentored a team of three junior scientists, providing guidance on product development and experiment execution, facilitating their growth and knowledge acquisition
  • Played a pivotal role in supporting the launch of a low-throughput single-cell assay, actively engaging in data analysis to ensure optimal results
  • Additionally, contributed to the preparation of the launch webinar in 2022, effectively presenting key findings and achievements.

Postdoctoral researcher

Broad Institute of Harvard and MIT
Cambridge
09.2017 - 07.2020
  • Conducted NGS analysis for a CRISPR-based barcode library for tracking cellular clones, including precise data filtering strategies, achieving accurate sgRNA-barcodes counts, calculated clonal fitness measurements resulting in 1/2500 false-positive rate in identifying high fitness clones
  • Developed a high-throughput barcoding system capable of handling 100,000 clones concurrently, showcasing scalability and efficiency in tracking large-scale, complex tumor system, demonstrating a remarkable 10-fold improvement in tracking drug-resistant clones compared to existing methods
  • Engineered and optimized a cutting-edge CRISPR-based methodology tailored specifically for oncology research, enabling precise tracking of clones at an unprecedented frequency as low as ~1/2,000.

Education

Postdoctoral researcher - Bioengineering

Broad Institute of Harvard and MIT
07.2020

Ph.D. - Cellular And Molecular Biology

Northwestern University
09.2017

Skills

  • Product development
  • NGS and single-cell-seq data analysis
  • Experimental design and execution

Awards

  • 10xer Award for exceptional work (2022)
  • Nominated for SALS Innovation Award (2022)
  • MWA Summer Pre-Doctoral Fellowship, American Heart Association (2016)

Publications

  • Jaffe D. B., Shahi P., Adams B. A., Chrisman A. M., Finnegan P. M., Raman N., Royall A. E., Tsai F et al. (2022) Functional antibodies exhibit light chain coherence. Nature
  • Feldman D
  • Funk L
  • Le A, Carlson R, Walton R, Leiken M, Tsai F, et al., (2021) Pooled genetic perturbation screens with image-based phenotypes. Nature Protocols (
  • Equal contributions)
  • And Tsai F
  • Et al., (2020) CloneRetriever: retrieval of rare clones from heterogeneous cell populations. BMC Biol (
  • Tsai F et al., (2017) Bim suppresses the development of SLE by limiting myeloid inflammatory responses. Journal of Experimental Medicine
  • (PCT/US2022/0l 7558) Drug screening methods (2022)
  • (WO 2019/089803) Methods and compositions for studying cell evolution (2019)
  • (PCT Application NO: PCT/US20/30286) Affinity-based multiplexing for live-cell monitoring of complex cell populations

Timeline

Scientist

10x Genomics
07.2020 - Current

Postdoctoral researcher

Broad Institute of Harvard and MIT
09.2017 - 07.2020

Postdoctoral researcher - Bioengineering

Broad Institute of Harvard and MIT

Ph.D. - Cellular And Molecular Biology

Northwestern University

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