Company Overview: Department of Veterinary Microbiology and Pathology
Department of Veterinary Microbiology and Pathology
Lab Assistant
Washington State University
Pullman, Washington
01.2022 - 12.2022
Company Overview: Paul G. Allen School for Global Animal Health
Paul G. Allen School for Global Animal Health
Postdoctoral Research Associate
Washington State University
Pullman, Washington
01.2020 - 12.2022
Company Overview: Department of Crop and Soil Sciences
Department of Crop and Soil Sciences
Technical Assistant
Washington State University
Pullman, Washington
01.2019 - 12.2020
Company Overview: Department of Crop and Soil Sciences
Department of Crop and Soil Sciences
Lab Assistant
University of Idaho
Moscow, Idaho
01.2019 - 12.2019
Company Overview: Department of Animal and Veterinary Science
Department of Animal and Veterinary Science
Research Assistant
Washington State University
Pullman, Washington
01.2017 - 12.2018
Company Overview: Department of Veterinary Microbiology and Pathology
Department of Veterinary Microbiology and Pathology
Head of the Zoology Department
University of Arts and Sciences in Elmarj, Libya
01.2009 - 12.2010
Company Overview: Department of Zoology
Department of Zoology
Faculty
University of Arts and Sciences in Elmarj, Libya
01.2007 - 12.2010
Company Overview: Department of Zoology
Department of Zoology
Lab Technician
University of Arts and Sciences in Elmarj, Libya
01.2001 - 12.2003
Company Overview: Department of Zoology
Department of Zoology
Department Coordinator
University of Arts and Sciences in Elmarj, Libya
01.2001 - 12.2003
Company Overview: Department of Zoology
Department of Zoology
Education
Ph.D. - Department of Veterinary Microbiology and Pathology
Washington State University
Pullman, WA
12-2018
M.S. - Department of Zoology
Garyounis University
Libya
07-2007
B. S. - Department of Zoology
Garyounis University
Libya
01.1999
Skills
Isolation of nucleic acids (DNA and RNA)
Bioinformatic analysis
Designing primers
PCR
Quantitative real-time PCR (Q-RT PCR)
Reverse Transcriptase PCR (RT-PCR)
Nested PCR
Separation of nucleic acids by agarose
Cloning
Transformation
Plasmid purification
Recombinant protein generation
Isolation of protein
Dot blot
Western blot analysis
Indirect ELISA
Immunofluorescence assays (IFA)
Immunohistochemistry
Wheat germination assay
Tick dissection
Hemolymph collection
Maintenance of Babesia parasite cultures
In vitro induction of Babesia bigemina sexual stage
Peptide injection
Examination and photography of Babesia parasite
Wild mice handling
Extract helminth parasites
Preparation of helminth parasite samples
Presentations
Babesia bigemina Tick Stage Specific Genes during Parasite Development within Adult Female Rhipicephalus microplus, Bohaliga, G. A., 2016, Department of Veterinary Microbiology and Pathology, Washington State University
Disrupting Babesia bigemina Transmission by Targeting Tick Stage Parasite Proteins, Bohaliga, G. A., 2017, Department of Veterinary Microbiology and Pathology, Washington State University
Babesia bigemina Gene Expression during Parasite Development within Adult Female Tick Rhipicephalus microplus, Bohaliga, G. A., Wendell C. Johnson, Naomi S. Taus, Hala E. Hussein, and Massaro W. Ueti, 2017, The 19th Annual College of Veterinary Medicine Student and PostDoctoral Research Symposium
Publications In Preparation
Insight to the gibberellin receptor, GID1 (GIBBERELLIN-INSENSITIVE DWARF 1) in wheat, Bohaliga, G. A. and Hauvermale, A. L.
Developing a new immunoassay specific for wheat alpha-amylase, Hauvermale, A. L., Matzke, C., Bohaliga, G. A., McCubbin, A., Pumphrey, M. and Steber, C. M.
Scholarships And Funding
Postdoctoral Research Associate, O.A. Vogel Wheat Research Fund, 01/2019, 12/2022, Two sides of the same germination coin: How the gibberellin (GA) hormone receptor regulates late maturity α-amylase (LMA) and preharvest sprouting (PHS) in wheat.
PhD Scholarship, Libyan Ministry of Education and Scientific Research (MOESR), 2012
Master Scholarship, Libyan Ministry of Education and Scientific Research (MOESR), 2003
Publications
Development of novel monoclonal antibodies to wheat alpha-amylases associated with grain quality problems that are increasing with climate change, Hauvermale, A. L., Matzke, C., Bohaliga, G., Pumphrey, M. O., Steber, C. M., & McCubbin, A. G., 2023, Plants, 12, 22, 3798
Identification of proteins expressed by Babesia bigemina kinetes, Bohaliga, G. A., Johnson, W. C., Taus, N. S., Hussein, H. E., Bastos, R. G., Suarez, C. E., ... & Ueti, M. W., 2019, Parasites & vectors, 12, 1, 1-9
Identification of a putative methyltransferase gene of Babesia bigemina as a novel molecular biomarker uniquely expressed in parasite tick stages, Bohaliga, G. A., Johnson, W. C., Taus, N. S., Hussein, H. E., Bastos, R. G., Suarez, C. E., ... & Ueti, M. W., 2018, Parasites & vectors, 11, 1, 1-13
Analysis of stage-specific protein expression during Babesia bovis development within female Rhipicephalus microplus, Johnson, W. C., Taus, N. S., Reif, K. E., Bohaliga, G. A., Kappmeyer, L. S., & Ueti, M. W., 2017, Journal of proteome research, 16, 3, 1327-1338
Volunteer Experience
Lab Assistant, Department of Veterinary Microbiology and Pathology, Washington State University, 01/2023, 12/2024
Lab Assistant, Paul G. Allen School for Global Animal Health, Washington State University, 2022
Lab Assistant, Department of Animal and Veterinary Science, University of Idaho, 2019
References
Upon request
Training
A training course in microbiology and parasitology, 2000, Benghazi Children's Hospital, Libya
Research
Adjunct Biology Instructor, Spokane Falls Community College, 01/2023, 12/2023
Postdoctoral Research Associate, Washington State University, Department of Crop and Soil Sciences, 01/2020, 12/2022
Technical Assistant, Washington State University, Department of Crop and Soil Sciences, 01/2019, 12/2020
Research Assistant, Washington State University, Department of Veterinary Microbiology and Pathology, 01/2017, 12/2018
Head of the Zoology Department, University of Arts and Sciences in Elmarj, Libya, Department of Zoology, 01/2009, 12/2010
Faculty, University of Arts and Sciences in Elmarj, Libya, Department of Zoology, 01/2007, 12/2010
Lab Technician, University of Arts and Sciences in Elmarj, Libya, Department of Zoology, 01/2001, 12/2003
Department Coordinator, University of Arts and Sciences in Elmarj, Libya, Department of Zoology, 01/2001, 12/2003
Courses Taught
General Zoology I, General Zoology II, lower invertebrate, higher invertebrate and parasitology classes (2007-2010)
Parasitology class (2008-2010)
Supervised and evaluated graduation projects for undergraduate students (2008-2009)
General Zoology lab (2010)
Timeline
Adjunct Biology Instructor
Spokane Falls Community College
01.2023 - Current
Lab Assistant
Washington State University
01.2023 - 12.2024
Lab Assistant
Washington State University
01.2022 - 12.2022
Postdoctoral Research Associate
Washington State University
01.2020 - 12.2022
Technical Assistant
Washington State University
01.2019 - 12.2020
Lab Assistant
University of Idaho
01.2019 - 12.2019
Research Assistant
Washington State University
01.2017 - 12.2018
Head of the Zoology Department
University of Arts and Sciences in Elmarj, Libya
01.2009 - 12.2010
Faculty
University of Arts and Sciences in Elmarj, Libya
01.2007 - 12.2010
Lab Technician
University of Arts and Sciences in Elmarj, Libya
01.2001 - 12.2003
Department Coordinator
University of Arts and Sciences in Elmarj, Libya
01.2001 - 12.2003
Ph.D. - Department of Veterinary Microbiology and Pathology