Overview
Work History
Education
Skills
Presentations
Publications In Preparation
Scholarships And Funding
Publications
Volunteer Experience
References
Training
Research
Courses Taught
Timeline
Generic

Gamila A. Rafea Bohaliga

Pullman,WA

Overview

25
25
years of professional experience

Work History

Adjunct Biology Instructor

Spokane Falls Community College
pullman, Washington
01.2023 - Current

Lab Assistant

Washington State University
Pullman, WA
01.2023 - 12.2024
  • Company Overview: Department of Veterinary Microbiology and Pathology
  • Department of Veterinary Microbiology and Pathology

Lab Assistant

Washington State University
Pullman, Washington
01.2022 - 12.2022
  • Company Overview: Paul G. Allen School for Global Animal Health
  • Paul G. Allen School for Global Animal Health

Postdoctoral Research Associate

Washington State University
Pullman, Washington
01.2020 - 12.2022
  • Company Overview: Department of Crop and Soil Sciences
  • Department of Crop and Soil Sciences

Technical Assistant

Washington State University
Pullman, Washington
01.2019 - 12.2020
  • Company Overview: Department of Crop and Soil Sciences
  • Department of Crop and Soil Sciences

Lab Assistant

University of Idaho
Moscow, Idaho
01.2019 - 12.2019
  • Company Overview: Department of Animal and Veterinary Science
  • Department of Animal and Veterinary Science

Research Assistant

Washington State University
Pullman, Washington
01.2017 - 12.2018
  • Company Overview: Department of Veterinary Microbiology and Pathology
  • Department of Veterinary Microbiology and Pathology

Head of the Zoology Department

University of Arts and Sciences in Elmarj, Libya
01.2009 - 12.2010
  • Company Overview: Department of Zoology
  • Department of Zoology

Faculty

University of Arts and Sciences in Elmarj, Libya
01.2007 - 12.2010
  • Company Overview: Department of Zoology
  • Department of Zoology

Lab Technician

University of Arts and Sciences in Elmarj, Libya
01.2001 - 12.2003
  • Company Overview: Department of Zoology
  • Department of Zoology

Department Coordinator

University of Arts and Sciences in Elmarj, Libya
01.2001 - 12.2003
  • Company Overview: Department of Zoology
  • Department of Zoology

Education

Ph.D. - Department of Veterinary Microbiology and Pathology

Washington State University
Pullman, WA
12-2018

M.S. - Department of Zoology

Garyounis University
Libya
07-2007

B. S. - Department of Zoology

Garyounis University
Libya
01.1999

Skills

  • Isolation of nucleic acids (DNA and RNA)
  • Bioinformatic analysis
  • Designing primers
  • PCR
  • Quantitative real-time PCR (Q-RT PCR)
  • Reverse Transcriptase PCR (RT-PCR)
  • Nested PCR
  • Separation of nucleic acids by agarose
  • Cloning
  • Transformation
  • Plasmid purification
  • Recombinant protein generation
  • Isolation of protein
  • Dot blot
  • Western blot analysis
  • Indirect ELISA
  • Immunofluorescence assays (IFA)
  • Immunohistochemistry
  • Wheat germination assay
  • Tick dissection
  • Hemolymph collection
  • Maintenance of Babesia parasite cultures
  • In vitro induction of Babesia bigemina sexual stage
  • Peptide injection
  • Examination and photography of Babesia parasite
  • Wild mice handling
  • Extract helminth parasites
  • Preparation of helminth parasite samples

Presentations

  • Babesia bigemina Tick Stage Specific Genes during Parasite Development within Adult Female Rhipicephalus microplus, Bohaliga, G. A., 2016, Department of Veterinary Microbiology and Pathology, Washington State University
  • Disrupting Babesia bigemina Transmission by Targeting Tick Stage Parasite Proteins, Bohaliga, G. A., 2017, Department of Veterinary Microbiology and Pathology, Washington State University
  • Babesia bigemina Gene Expression during Parasite Development within Adult Female Tick Rhipicephalus microplus, Bohaliga, G. A., Wendell C. Johnson, Naomi S. Taus, Hala E. Hussein, and Massaro W. Ueti, 2017, The 19th Annual College of Veterinary Medicine Student and PostDoctoral Research Symposium

Publications In Preparation

  • Insight to the gibberellin receptor, GID1 (GIBBERELLIN-INSENSITIVE DWARF 1) in wheat, Bohaliga, G. A. and Hauvermale, A. L.
  • Developing a new immunoassay specific for wheat alpha-amylase, Hauvermale, A. L., Matzke, C., Bohaliga, G. A., McCubbin, A., Pumphrey, M. and Steber, C. M.

Scholarships And Funding

  • Postdoctoral Research Associate, O.A. Vogel Wheat Research Fund, 01/2019, 12/2022, Two sides of the same germination coin: How the gibberellin (GA) hormone receptor regulates late maturity α-amylase (LMA) and preharvest sprouting (PHS) in wheat.
  • PhD Scholarship, Libyan Ministry of Education and Scientific Research (MOESR), 2012
  • Master Scholarship, Libyan Ministry of Education and Scientific Research (MOESR), 2003

Publications

  • Development of novel monoclonal antibodies to wheat alpha-amylases associated with grain quality problems that are increasing with climate change, Hauvermale, A. L., Matzke, C., Bohaliga, G., Pumphrey, M. O., Steber, C. M., & McCubbin, A. G., 2023, Plants, 12, 22, 3798
  • Identification of proteins expressed by Babesia bigemina kinetes, Bohaliga, G. A., Johnson, W. C., Taus, N. S., Hussein, H. E., Bastos, R. G., Suarez, C. E., ... & Ueti, M. W., 2019, Parasites & vectors, 12, 1, 1-9
  • Identification of a putative methyltransferase gene of Babesia bigemina as a novel molecular biomarker uniquely expressed in parasite tick stages, Bohaliga, G. A., Johnson, W. C., Taus, N. S., Hussein, H. E., Bastos, R. G., Suarez, C. E., ... & Ueti, M. W., 2018, Parasites & vectors, 11, 1, 1-13
  • Analysis of stage-specific protein expression during Babesia bovis development within female Rhipicephalus microplus, Johnson, W. C., Taus, N. S., Reif, K. E., Bohaliga, G. A., Kappmeyer, L. S., & Ueti, M. W., 2017, Journal of proteome research, 16, 3, 1327-1338

Volunteer Experience

  • Lab Assistant, Department of Veterinary Microbiology and Pathology, Washington State University, 01/2023, 12/2024
  • Lab Assistant, Paul G. Allen School for Global Animal Health, Washington State University, 2022
  • Lab Assistant, Department of Animal and Veterinary Science, University of Idaho, 2019

References

Upon request

Training

A training course in microbiology and parasitology, 2000, Benghazi Children's Hospital, Libya

Research

  • Adjunct Biology Instructor, Spokane Falls Community College, 01/2023, 12/2023
  • Postdoctoral Research Associate, Washington State University, Department of Crop and Soil Sciences, 01/2020, 12/2022
  • Technical Assistant, Washington State University, Department of Crop and Soil Sciences, 01/2019, 12/2020
  • Research Assistant, Washington State University, Department of Veterinary Microbiology and Pathology, 01/2017, 12/2018
  • Head of the Zoology Department, University of Arts and Sciences in Elmarj, Libya, Department of Zoology, 01/2009, 12/2010
  • Faculty, University of Arts and Sciences in Elmarj, Libya, Department of Zoology, 01/2007, 12/2010
  • Lab Technician, University of Arts and Sciences in Elmarj, Libya, Department of Zoology, 01/2001, 12/2003
  • Department Coordinator, University of Arts and Sciences in Elmarj, Libya, Department of Zoology, 01/2001, 12/2003

Courses Taught

  • General Zoology I, General Zoology II, lower invertebrate, higher invertebrate and parasitology classes (2007-2010)
  • Parasitology class (2008-2010)
  • Supervised and evaluated graduation projects for undergraduate students (2008-2009)
  • General Zoology lab (2010)

Timeline

Adjunct Biology Instructor

Spokane Falls Community College
01.2023 - Current

Lab Assistant

Washington State University
01.2023 - 12.2024

Lab Assistant

Washington State University
01.2022 - 12.2022

Postdoctoral Research Associate

Washington State University
01.2020 - 12.2022

Technical Assistant

Washington State University
01.2019 - 12.2020

Lab Assistant

University of Idaho
01.2019 - 12.2019

Research Assistant

Washington State University
01.2017 - 12.2018

Head of the Zoology Department

University of Arts and Sciences in Elmarj, Libya
01.2009 - 12.2010

Faculty

University of Arts and Sciences in Elmarj, Libya
01.2007 - 12.2010

Lab Technician

University of Arts and Sciences in Elmarj, Libya
01.2001 - 12.2003

Department Coordinator

University of Arts and Sciences in Elmarj, Libya
01.2001 - 12.2003

Ph.D. - Department of Veterinary Microbiology and Pathology

Washington State University

M.S. - Department of Zoology

Garyounis University

B. S. - Department of Zoology

Garyounis University
Gamila A. Rafea Bohaliga