Hussien F. Alameldin
Research Specialist - Fixed Term
East Lansing,MI
Summary
Postdoctoral Fellow with 6 years in fellowship at the DOE-Plant Research Laboratory at Michigan State University. Organized with research and presentations educating students, peers and superiors on research analysis. Published research findings through peer-reviewed articles and analyzed results tailoring to possible future applications.
I am dedicated to exploring the molecular mechanisms that underlie the resilience of plants to various stress factors, both biotic and abiotic. My background in basic biology, combined with my expertise in biotechnology, computational biology, and molecular biology, provides a strong foundation for our research. With a broad range of hands-on experience working with diverse plant species, such as Arabidopsis, Rice, Corn, Soybean, Wheat and others, I am well-versed in techniques like metabolic engineering, genotyping, high- throughput phenotyping, disease resistance screenings, and conventional breeding. Additionally, I have experience working with large-scale datasets, including genomic, transcriptomic, and metabolomic data, and am proficient in using R and Python to analyze these data. Considerate Biotechnology professional experienced in putting quality control procedures to work and lending hand with creating effective research protocols. Polished writer successful at delivering documentation compiled from research subjects and studies. Postdoctoral Fellow performing in-depth research regarding Genomics data. Managed laboratories and enforced safety procedures and proper use of personnel protective equipment. Followed and enforced all institutional regulations and federal guidelines. Orderly and focused researcher dedicated to innovative, dynamic and organized work. Successful at maintaining accuracy and productivity through long and short-term projects with proven history of efficiency.
Overview
16
16
years of professional experience
Work History
Postdoctoral Research Associate
Michigan State University, US Department of Energy
09.2016 - Current
- Developed high throughput data collection strategies and monitored data quality to study the effect of dynamic light environment on plants for optimal research results.
- Performed research into study transcriptome data coupled with high-throughput phenotypic data to increase knowledge and to provide valuable contributions.
- Collaborated with leadership team in high throughput phenotyping, Bioinformatics, and Biochemistry to identify relevant questions and determine best methods of collection.
- Directed, guided and delegated tasks to teams and developed project schedules, resource requirements and timelines to maintain productivity on goals and objectives.
- Contributed to scientific discussions with peers and management personnel.
- Determined areas of research to increase knowledge in particular field.
- Managed project risk by identifying, quantifying and monitoring potential threats.
- Generated and upload assay data and communicated results and reports to multi-disciplinary project teams to influence progression in discovery and development.Publishedli>published results in peer reviewed journals, and presented in specialized scientific meetings.
- Analyzed and interpreted patterns and trends.
- Synthesized and utilized key information from proposals, technical reports and publications to achieve objectives.
- Sorted all phenotypic, molecular, Biochemistry and RNAseq data into well-organized formats and transformed into figures.
- Achieved project objectives by troubleshooting experiments and testing practical and creative solutions.
- Interpreted data analysis results to draw inferences and conclusions.
- Utilized statistical packages to analyze data and create visualizations.
- Maintained accurate records of research findings and provided statistical analysis of data results.
- Enforced laboratory safety protocols and enabled safe work environment.
- Authored professional scientific papers for publishing in peer-reviewed journals.
- Reviewed and analyzed historical and emerging literature to develop and practice new technologies.
- Collaborated with High-Throughput imaging and Bioinformatics professionals to advance research and gain deeper understanding of topics.
- Gathered, documented, analyzed and presented findings.
- Maintained clean, organized and well-stocked laboratory environments.
- Pursued independent and complementary research interests.
Postdoctoral Research Associate
Biofuel and Biopharmaceutical Crop Genetic Engineering Laboratory, Michigan State University
01.2014 - 08.2016
- Designed research projects and alternative approaches and discussed results with professor.
- Contributed to and actively participated in research conception, design and execution to address defined problems.
- Worked to improve collective knowledge of Biotechnology. Maintained accurate records of research findings and provided statistical analysis of data results.
- Troubleshot and developed experimental tools to deal with ever-evolving technology.
- Attended research seminars and collegiate meetings to discover and discuss new technologies and research.
- Conducted independent research within inclusive environment.
- Assisted teaching and instructing students alongside other professors.
- Wrote and published peer-reviewed articles concerning findings and highlighted possible applications for findings.
Senior Research Assistant
Bioinformatics, Genomics Lab
05.2007 - 09.2013
- Projects: - Identification of biotic stress-related genes from wheat using microarray technology
- Identification of some salt stress-resistant genes on rice using microarray
- Analyzed data using limma Package
- Development of drought-tolerant bread wheat via biotechnological approaches
- Development of stored insect-free wheat via metabolic engineering
- Development of fungal-resistant bread wheat using biotechnological approaches
- Skill/techniques: Preparation of cDNA library, performed microarray hybridization, scanning slides, and analyzed data using limma package, gene cloning, tissue culture, plant transformation, Biolistic bombardment-based transformation, gene expression (RT-PCR), western blotting molecular cloning, PCR, gel electrophoresis, field and growth chamber experience, risk assessment for biosafety evaluation, and data analysis.
- Prioritized projects and assignments assigned by mentor and leadership to complete research objectives on time.
- Maintained and calibrated various types of lab equipment.
- Maintained up-to-date records of research activities and results for future reference.
- Analyzed discussion and creation of project objectives, proposals and budgets to meet funding criteria.
- Performed statistical, qualitative, and quantitative analysis.
- Recorded experimental findings in laboratory notebook to analyze and interpret experimental findings.
- Maintained up-to-date records of research activities and results for future reference
D. Visiting Scholar
Biofuel and Biopharmaceutical Crop Genetic Engineering Laboratory, Michigan State University
03.2011 - 05.2012
- Study the effect of knockdown of the PhyB gene in Japonica Rice plants
- Skill/techniques: Gene cloning, tissue culture, plant transformation, Biolistic bombardment-based and Agrobacterium transformation, gene expression (qRT-PCR), western blotting molecular cloning, PCR, gel electrophoresis.
Education
Ph.D. - Genetics
Michigan State University & Ain Shams University
East Lansing, MI12.2012
Master of Science - Genetics
Ain Shams University
Cairo, Egypt 06.2007
Bachelor of Science - Genetics
Ain Shams University
Cairo, Egypt05.2000
Skills
- Gene discovery
- isolation and purification of nucleic acids
- Molecular Genotyping techniques
- Designing, conducting, and analyzing qPCR assays
- Plant transformation, metabolic engineering, and tissue culture (Maize, Wheat, Rice, Soybean, Stevia, Sugarcane, and more)
- Experience in breeding and cross-fertilizing various plants (Arabidopsis, Wheat, Corn, Rice, and others) in field and laboratory settings
- High-throughput phenotyping and data analysis
- Gene cloning and transformation
- CRISPR-Cas9 experimental design
- Nucleic acid and protein hybridization techniques
- Gas Chromatography-Mass Spectrometry (GC-MS)
- Biochemical and enzymatic analysis
- Quantifying pigments using various methods
- Screening and analyzing plant-pathogen interactions
- Analyzing differential expression using RNAseq data
- Microarray data analysis
- Transcriptome assembly and functional annotation using different pipelines
- Genome Sequencing assembly and annotation using MAKER2
- Variant calling
- GitHub for collaborative software development
- Skilled in R and Python programming languages
- Research Protocols
- Supply Restocking
- Data Analysis
- Findings Presentations
- Genome Sequencing
- Supply Ordering
- Critical Thinking
- Gene Regulation
- Scientific Writing
- Diagnostic Solutions
- Project Coordination
- Equipment Maintenance
- DNA Cloning
- Experimental Design
- Laboratory Safety Protocols
- Campus Operations
- Library Instruction
- Grant Proposals
Work Availability
monday
tuesday
wednesday
thursday
friday
saturday
sunday
morning
afternoon
evening
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Quote
There’s no shortage of remarkable ideas, what’s missing is the will to execute them.
Seth Godin
Timeline
Postdoctoral Research Associate
Michigan State University, US Department of Energy
09.2016 - Current
Postdoctoral Research Associate
Biofuel and Biopharmaceutical Crop Genetic Engineering Laboratory, Michigan State University
01.2014 - 08.2016
D. Visiting Scholar
Biofuel and Biopharmaceutical Crop Genetic Engineering Laboratory, Michigan State University
03.2011 - 05.2012
Senior Research Assistant
Bioinformatics, Genomics Lab
05.2007 - 09.2013
Ph.D. - Genetics
Michigan State University & Ain Shams University
Master of Science - Genetics
Ain Shams University
Bachelor of Science - Genetics
Ain Shams University
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