Isabella M. Rosa
Philadelphia,PA
Summary
Enthusiastic biochemistry Master's student preparing to graduate in the Spring. Excels in communication both written and oral, critical thinking, and working both independently and collaboratively. Skilled in biochemical techniques with a willingness to learn on the job.
Overview
7
7
years of professional experience
Work History
Biochemistry Graduate Student
Manhart Lab, Temple University
Philadelphia, USA
01.2019 - Current
- Research topic: Elucidating the binding mode of Mlh1-Pms1 to DNA in the mismatch repair pathway.
- Advisor: Dr. Carol Manhart
- Managing a project focused on characterizing the DNA-binding interaction of mismatch repair endonuclease Mlh1-Pms1.
- Performing biochemical and molecular techniques, including but not limited to column chromatography (FPLC), gel electrophoresis, in vitro enzymatic assays, PCR, centrifugation, cell culture, and spectrophotometry.
- Mentoring and training graduate and undergraduate laboratory members.
- Maintaining laboratory organization, laboratory instrumentation and equipment, and inventory upkeep.
Teaching Assistant
Department of Chemistry, Temple University
Philadelphia, USA
09.2019 - 12.2021
- Teaching biochemistry and chemistry undergraduates theoretical (Recitation) and practical (Laboratory) chemical concepts
- Research Techniques in Biochemistry Laboratory (CHEM 4496) – Spring 2025
- Biochemistry I Recitation (CHEM 4401) – Fall 2020-Fall 2021
- General Chemistry I Recitation (CHEM 1031) – Fall 2021
- Applications of Biochemistry Recitation (CHEM 3401) – Fall 2020-Spring 2021
- Techniques of Chemical Measurement Laboratory (CHEM 3105) – Spring 2020
- Techniques of Chemical Measurement, Recitation (CHEM 3103) – Fall 2019-Spring 2020
Graduate Student Volunteer
Department of Chemistry, Temple University
Philadelphia, USA
09.2024 - 05.2025
- Graduate student volunteer for the Buckets and Beakers Science Outreach Program.
- Teaching 5th-grade students chemical concepts through hands-on activities.
Chair of Biochemistry Supergroup
Department of Chemistry, Temple University
Philadelphia, USA
06.2022 - 05.2024
Co-Chair of the Council on Student Involvement (CSI)
Department of Chemistry, Temple University
Philadelphia, USA
09.2020 - 05.2022
Co-chair of Graduate Recruitment Committee Student Faction
Department of Chemistry, Temple University
Philadelphia, USA
09.2020 - 05.2022
Biochemistry Undergraduate Student
Manhart Lab, Temple University
Philadelphia, USA
01.2018 - 01.2019
- Research topic: Investigating the role of ATP activity in Mlh1-Pms1 DNA mismatch repair mechanism
- Advisor: Dr
- Carol Manhart
- Performing biochemical and molecular techniques including gel electrophoresis, in vitro enzymatic assays, and PCR
Education
Masters of Science (MS) - Chemistry
Temple University
Philadelphia, PA05-2025
Bachelor of Science (B.S.) - Biochemistry
Temple University
Philadelphia, PA01.2019
Skills
- Written and oral communication of scientific concepts
- Project management
- Biochemical and analytical chemistry techniques
- Critical thinking
- Willingness to learn
- Data management
- Teamwork
- Organization
- Leadership and mentorship
- Microsoft Office Suite
Publications
Scott J. Witte, Isabella M. Rosa, Bryce W. Collingwood, Jonathan M. Piscitelli, Carol M. Manhart, The mismatch repair endonuclease MutLα tethers duplex regions of DNA together and relieves DNA torsional tension, Nucleic Acid Research, 02/25/23
References
Available upon request
Presentations
- The endonuclease Mlh1-Pms1 uses strand capture activity to facilitate DNA mismatch repair, Rosa, I. M., Sakinejad, Y. S., Manhart, C.M., Genomic Instability: Discovery to Personalized Medicine Symposium, Philadelphia, PA, 11/03/23
- The endonuclease Mlh1-Pms1 uses strand capture activity to facilitate DNA mismatch repair, Rosa, I. M., Sakinejad, Y. S., Manhart, C.M., R3 20th Anniversary Symposium, Ithaca, NY, 06/02/23
- Investigating the Role of ATP in Mlh1-Pms1 mediated DNA mismatch repair, Rosa, I. M., Manhart, C. M., Alliance for Diversity in Science & Engineering, Young Researcher’s Conference, Philadelphia, PA, 04/27/19
- Investigating the Role of ATP in Mlh1-Pms1 mediated DNA mismatch repair, Rosa, I. M., Manhart, C. M., The Symposium for Undergraduate Research and Creativity, Philadelphia, PA, 04/11/19
References
References available upon request.
Timeline
Graduate Student Volunteer
Department of Chemistry, Temple University
09.2024 - 05.2025
Chair of Biochemistry Supergroup
Department of Chemistry, Temple University
06.2022 - 05.2024
Co-Chair of the Council on Student Involvement (CSI)
Department of Chemistry, Temple University
09.2020 - 05.2022
Co-chair of Graduate Recruitment Committee Student Faction
Department of Chemistry, Temple University
09.2020 - 05.2022
Teaching Assistant
Department of Chemistry, Temple University
09.2019 - 12.2021
Biochemistry Graduate Student
Manhart Lab, Temple University
01.2019 - Current
Biochemistry Undergraduate Student
Manhart Lab, Temple University
01.2018 - 01.2019
Masters of Science (MS) - Chemistry
Temple University
Bachelor of Science (B.S.) - Biochemistry
Temple University
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