Molecular Biology: Polymerase chain reaction (PCR), gene cloning, protein expression and purification, DNA/RNA purification, DNA gene analysis, Bacterial strain construction by gene manipulation/deletion and some cell culture
Biochemistry: Analysis of bacterial lipids by Matrix-Assisted Laser/Desorption Ionization (MALDI) mass spectrophotometry
BECC-engineered live-attenuated Shigella vaccine candidates display reduced endotoxicity with robust immunogenicity in mice.
Sherman ME, Michalski J, Das S, Yang H, Chandrasekaran L, O'Meara TR, Dowling DJ, Levy O, Barnoy S, Venkatesan M, Ernst RK. Vaccine. 2025 doi:10.1016j.vaccine.2025.126779.
Genomic diversity of non-diarrheagenic fecal Escherichia coli from children in sub-Saharan Africa and south Asia and their relatedness to diarrheagenic E. coli.
Hazen TH, Michalski JM, Tennant SM, Rasko DA. Nat Commun. 2023 doi: 10.1038/s41467-023-36337-y.
H-NST induces LEE expression and the formation of attaching and effacing lesions in enterohemorrhagic Escherichia coli.
Levine JA, Hansen AM, Michalski JM, Hazen TH, Rasko DA, Kaper JB. PLoS One. 2014 Jan 21;9(1):e86618. doi: 10.1371/jo