Meticulous and analytical Researcher with four plus years of educational and hands-on experience in Biopharma. Adaptive team player with in-depth knowledge of mammalian cell culture, maintaining and differentiating hESCs/iPSCs. Specializing in cell-based assays, such as flow cytometry, CX5 High-Content Screening (HCS) Platform, fluorescent microscopy, ICC staining, IHC/IF assays, RT-qPCR, and DNA/RNA purification. Excellent communication skills and complying with SOPs. Ready to apply knowledge and experience to meet company goals.
Hi, I’m
JOAN N
Science Researcher
Oakland,CA
Summary
Overview
4
years of professional experience
1
Language
Work History
Neurona Therapeutics
Research Associate II
08.2020 - 02.2023
Job overview
- Cultured and maintained various cell lines, including hESCs, K562, NK cells, and HEK-293T
- Cultured hESCs which we differentiated into cortical interneurons (cINs)
- Cultured the hESCs at different scales (flasks, bottles, stacks)
- Verified cell composition using molecular marker analysis (flow cytometry, CX5 High-Content Screening (HCS) Platform, fluorescent microscopy, ICC staining, RT-qPCR, DNA/RNA purification) for in-process QC
- Derived modified, pure, stable human pluripotent stem cell lines by single cell subcloning protocols
- Developed new assays for measuring cell cytotoxicity using flow cytometry
- Maintained accurate records of all methods and research conducted and wrote in-depth reports
- Maintained laboratory inventories for antibodies, cells, reagents, and consumables
- Performed routine histology such as preparing and sectioning tissues and developed IHC/IF assays
- Performed complex troubleshooting and assisted in leading initiatives to solve challenging scientific problems with assays or instrumentation
Amgen
CIRM Bridges Scholar
09.2019 - 08.2020
Job overview
- Cultured induced pluripotent stem cells (iPSCs) for further differentiation into cardiomyocytes to develop an integrated platform to study atrial fibrillation (Afib)
- Carried out sample processing workflows to characterize the cardiomyocytes including western blotting, RT-qPCR, DNA/RNA purification, ICC staining, and developed IHC/IF assays
- Developed a siRNA transfection protocol of our ESCs
- Performed flow cytometry to run various assays as well as characterize and sort cells
- Developed, optimized, and carried out various qPCR assays
- Set up equipment, organized inventory and maintained facilities
- Gathered, arranged, and corrected research data (using PowerPoint, FlowJo, and Excel) to create representative graphs and charts highlighting results for presentations
- Conducted extensive literature reviews to inform research direction and stay current on emerging trends in the field
Education
San Jose State UniversitySan Jose, CA
Master of Science from Stem Cells and Biotechnology
05.2020
University Overview
Kennesaw State UniversityKennesaw, GA
Bachelor of Science from Biochemistry
12.2017
University Overview
Skills
- Aseptic cell culture
- Microscopy
- Multicolor flow cytometry
- Gene editing (CRISPR, AAV)
- Quality control (QC)
- Lab Operations
- Research presentation
- Histotechnology
- FlowJo
- GraphPad Prism
- SnapGene
- Jupyter Notebook
- Data Interpretation
- Research and analysis
- Quantitative Research
- Biostatistics
- MS Office
- Google Suite
- Slack
INTERESTS
INTERESTS
- Hiking, Reading, Jogging, Movies, Cooking
Quote
Far and away the best prize that life offers is the chance to work hard at work worth doing.
Timeline
Research Associate II
Neurona Therapeutics
08.2020 - 02.2023
CIRM Bridges Scholar
Amgen
09.2019 - 08.2020
San Jose State University
Master of Science from Stem Cells and Biotechnology
Kennesaw State University
Bachelor of Science from Biochemistry
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