Breakfast
Joseph Flounlacker
Associate Scientist
Philadelphia,Pennsylvania
Summary
Associate Scientist proficient in training GMP associates and effectively communicating with client and multiple departments. Talented individual educated in safety procedures, downstream viral vector, cell therapy and gene therapy processes. Leverages depth of laboratory experience to perform complex assays and testing.
Overview
19
19
years of professional experience
Work History
Associate Scientist
Wuxi Advanced Therapies
06.2018 - Current
- Downstream processing specialist on client AAV and Lenti processes involving client scale up to GMP processing for viral vectors.
- Supported 5-7 client projects per year by generating scalable data for clarification, chromatography, TFF, Ultracentrifugation, and sterile filtration from 100mL to 200L processing parameters.
- Organized feasibility studies, verification runs, and pilot production. Supported GMP processing for Manufacturing phase
- Provided analytics such as weekly flow cytometry for Lenti and AAVx ELISA data (2/month) for AAV for all clients in Process Development.
- Familiar with ddPCR, qPCR, SEC, and DLS for rapid titer analytics
- Wrote Batch Records for GMP use for all downstream operations
- Material and Ordering specialist in my group with SAP B1 and Ariba buying. Procurement and system trainer within Process Development
Manufacturing Associate 2
Wuxi Advanced Therapies
01.2015 - 06.2018
- Subject Matter Expert in multiple projects related(JCAR, Cell Therapies involving allogenic and autogenic donation) to human tissue donor, tissue sorting, and stem cell platform technology.
- Cross-trained on projects in commercial Gene Therapeutics wing
- Leadership role in growing biotechnology company with client-focused approach
- Equipment team member and NCE compliance officer
- Updated and generated batch records and SOPs for GMP processing
- Wrote client reports for 10 cell banks a year
Laboratory Technician Supervisor
TAG Pharmacy
10.2011 - 08.2014
- Started Philadelphia branch of California-based sinus medication conglomerate
- Supervised 4 laboratory technicians in compounding and dispensing specialized sinus medication
- Transformed normal pharmacy cleanroom into 797 compliant sterile cleanroom
- Handled approximately 100 orders per day in addition to compounding specialized medications to fill orders
- Strict adherence to GMP/GLP guidelines regarding 797 compounding pharmacies
Research Technician 3
Children's Hospital Of Pittsburgh
09.2004 - 06.2011
- Managed care and maintenance for over 300 separate cages housing population of 1,000 mice
- Studied central tolerance theory using tissue-specific deletion of insulin gene in thymus
- Skilled with PCR and qRT-PCR; bred, weaned, and typed over 10,000 multiple strain transgenic rodents
- Designed and implemented experiments using mice, such as intraperitoneal glucose tolerance test (IPGTT) and insulin tolerance test (ITT)
- Responsible for managing lab inventory and requisitioning supplies (~ $3,000/week)
- Interdepartmental Liaison to UMPC internal regulation group, DLAR
Education
Bachelor of Science - Chemistry
University of Pittsburgh
Pittsburgh, PA 05.2001 -
Skills
Training Expert
Assay Development
Material and Ordering Specialist(SAP B1 and Ariba)
Sample Transfer Systems(LIMS)
Interests
Dogs
Timeline
Associate Scientist
Wuxi Advanced Therapies
06.2018 - Current
Manufacturing Associate 2
Wuxi Advanced Therapies
01.2015 - 06.2018
Laboratory Technician Supervisor
TAG Pharmacy
10.2011 - 08.2014
Research Technician 3
Children's Hospital Of Pittsburgh
09.2004 - 06.2011
Bachelor of Science - Chemistry
University of Pittsburgh
05.2001 -