Judith D. Krigman BA MAT MA
Brandon,FL
Summary
Dedicated and versatile Research Associate and Laboratory Manager with fifteen years of experience spanning multiple facets of research, animal husbandry, IACUC, laboratory, and administrative roles. IACUC and IRB experience, and have taught confocal and multiphoton microscopy. Proficient in translating complex scientific concepts into accessible content for diverse audiences. Demonstrated ability to excel in collaborative environments utilizing data analysis, graphic design, and written communication to assist project coordination. Adept at employing various technical tools and platforms to document and communicate research findings effectively.
Overview
14
14
years of professional experience
1
1
Language
Work History
Senior Research Associate
Florida Stroke Registry/ University Of Miami
10.2022 - Current
- Program Contribution : Contributed to Florida Stroke Registry (FSR) and Paul Coverdell program by attending and documenting meetings, developing reports, and designing graphics for various reports to FDOH, stakeholders, and FSR website.
- Report Compilation: Responsible for compiling and writing monthly, semi-annual, and annual reports, utilizing Adobe InDesign to prepare Annual Stakeholder reports and integrating technical writing with graphic design.
- Graphic Design and Presentation : Regularly employed Canva, Krita, PowerPoint, and Adobe Suite to advance projects.
- Website Management : Monitored publication, coalition, and education sections of FSR website, researching, installing, and utilizing WordPress to prepare and share quarterly and annual performance metrics on stakeholders' dashboards.
- Survey Distribution and Data Analysis: Managed distribution, tracking, and collection of annual REDCap Hospital Inventory survey and hospital abstraction, performing extensive data analysis to create accessible tables and dashboards.
- Data Proficiency: Proficient in using Excel with pivot tables for tracking daily activities and specialized projects, with ongoing initiative to learn Microsoft Power BI.
- Event Organization: Actively participated in organizing and preparing annual meetings sponsored by FSR, including fine-tuning speaker slides, researching venues, taking detailed notes, and interacting with stakeholders.
- Post-Event Reporting: Compiled and produced high-quality final reports for stakeholders using written and visual media post-event.
Research Biologist
Bruce W. Carter VA Medical Center
07.2022 - 10.2022
- Software Identification: Utilized TRM to identify VA-approved software.
- SOP Development: Wrote SOPs for equipment used in a PTSD sleep study and collaborated with the University of Pennsylvania team.
- Data Entry and Protocol Learning: Entered data into ERDSP and 10-250 IRB forms and initiated learning of IRB protocols and practices.
- Training and Compliance: Completed IATA training for hazardous chemicals and continued CITI training for Good Laboratory Practices.
Research Associate 2/Lab Manager
Ohio State University: Nuo Sun Lab
08.2018 - 07.2022
- Performed Manuscript and Grant Editing: Improved grammar and clarity of papers and grants, leading to high scores of 1% and 15%. Researched, wrote, and submitted a review on the endoplasmic reticulum and mitochondria relationship to Frontiers.
- Lab Setup and Managements: As the first person hired, was responsible for coordinating with vendors, procuring and purchasing equipment, and locating and managing inventory utilizing Workday.
- Laboratory Coordination: Set up equipment, organized inventory, and maintained facilities to ensure efficient operation.
- Regulatory Compliance: Worked with EHS, IACUC, the University's Control Substance Program, and other regulatory affairs groups to ensure lab compliance. Responsible for documentation and filing of regulatory paperwork.
- Mouse Colony Management: Managed a 150+ cage mouse colony with multiple genotypes, including tagging, breeding, weaning, and performing I.P. and subcutaneous injections.
- Surgical Coordination: Collaborated with surgical teams and veterinary staff on research projects, identifying animal care issues, and writing corrective action protocols.
- Imaging and Analysis: Performed and analyzed echocardiograms SAX and PSLAX using the VEVO 3100 system to track cardiac changes in adult mice and pups.
- Data Management: Created and maintained various Excel spreadsheets for mouse colony management, supply, echocardiograms, and tumor analysis with conditional formatting for early removal criteria.
- Safety and Compliance: Monitored and documented safety standards to verify compliance, maintaining EHS, chemical logs, and controlled substances.
- Team Leadership: Provided support and guidance to Ph.D. students and undergraduate volunteers and ensured they followed laboratory protocols and safety procedures.
Research Associate 1
Ohio State University: CMIF
11.2017 - 08.2018
- Microscopy: Utilized Olympus FV1000 confocal/multiphoton and Nikon A1R for small animal imaging tailored to individual research groups. Conducted SEM and TEM for small projects. Responsible for daily equipment review to ensure usability.
- Protocol Development: Researched and wrote an IACUC-approved protocol for cranial window implants in mice, acquiring skills to implant small 3mm windows in Bregma.
- Educational Resource: Created PowerPoint templates and teaching manuals for new Olympus FV1000 multiphoton and Nikon A1R users. These were used when teaching microscopy.
- Imaging Sessions: Conducted one-on-one confocal and multiphoton microscopic imaging sessions for research groups.
- Billing and Accounting: Reviewed monthly CMIF department billing synchronization in FOM software, identifying and resolving accounting issues.
Technical Customer Service Representative
LabCorp
01.2016 - 08.2017
- Call Center Operations: Averaged 96 daily calls while maintaining a high call quality, including technical knowledge, courteous communication, thoroughness, schedule adherence, and daily reporting. Ensured HIPAA compliance.
- Specimen and Test Identification: Located specimens, identified tests using a six-digit test code, and explained various testing methodologies to callers. Responsible for coordinating pick-ups and delivery for drivers, often averaging less than 30 seconds per call.
- Technical Assistance: Provided technical assistance for various medical disciplines, including blood draws, viral/bacterial/fungal cultures, PCRs, and Western Blots.
- Sample Transfer Documentation: Documented and followed sample transfers within LabCorp and other facilities, reporting to other labs, clients, and supervisors, noting changes or anomalies within company guidelines.
- First-Call Resolution: Improved first-call resolution rates by troubleshooting and diagnosing customer concerns.
- Customer Satisfaction: Enhanced customer satisfaction by promptly addressing and resolving technical issues.
- Billing Estimates and Complaint Resolution: Provided billing estimates to accounts and patients. Resolved patient, provider, and draw center complaints.
Receiving Technician
Mérieux NutriSciences
08.2015 - 01.2016
- Sample Handling and Analysis: Received processed and raw foods for biological and chemical analysis and environmental samples from manufacturers in the food industry.
- LIMS System Management: Logged several hundred samples a day into the Laboratory Information Management System (LIMS) based on test type and description.
- Issue Resolution: Resolved and located missing or unclear testing or sample information via phone call or correspondence.
- Data Accuracy: Daily proofing and documenting of errors in orders and data entry in the LIMS system with less than a 0.5% error rate..
- Sample Outsourcing Management: Managed outsourcing of samples to other testing facilities, including generating broker reports, locating samples, packing samples, and shipping 30-plus boxes using the FedEx system.
Research Associate 2
Ohio State University: Department of Radiation and Oncology/Department of Molecular Virology, Immunology, and Medical Genetics
03.2013 - 08.2015
- Cancer Research: Assisted in implantation and radiation studies for prostate, glioblastoma, and intestinal cancers. Tumors were implanted using stereotactic surgery into the brain or subcutaneously onto the hip.
- Regulatory Actions: Maintained and reported records to IACUC.
- Radiation Tracking and Analysis: Managed radiation schedules for tumors and tracked their progress over time, including recovery, measurement, and preparation for cell culture or DNA/RNA extraction post-mortem.
- Mouse Colony Management: Breeding, tagging, and genotyping a large colony of MSH2 mice (200+ cages).
- Injections: Administered subcutaneous and IP injections'
- Cost Analysis and Supply Management: Researched and developed cost analyses for the MSH2 mouse project and maintained or ordered supplies as needed.
- Lab Organization: Set up equipment, organized inventory, and maintained facilities.
Research Technician II
University of Pittsburgh: Department of Pharmacology and Chemical Biology
06.2010 - 03.2013
- Mouse Colony Management: Managed 30-cage NHERF mouse colony, including breeding logs, tagging, genotyping, obtaining tissue samples for primary cell culture, and proper documentation.
- Cell Line Maintenance: Generated and maintained multiple cell lines using transfection and antibiotic selection, confirmed via Western blot and immunofluorescence. Performed primary cell retrieval and propagation.
- Molecular Techniques: Performed PCR, gel purification, restriction/alkaline digests, and ligation. Prepared solutions for Ph.D. Students.
- Reagent Preparation: Prepared reagents and solutions following standard formulas and molarity for media, agar plates, and other needed reagents.
- Plasmid Transformation: Transformed and purified plasmids using Qiagen and alkaline lysis methods.
- Database and Inventory Management: Established Access and Excel databases for plasmids, antibodies, and supplies.
- Safety Compliance: Maintained a safe and organized lab environment, ensuring adherence to safety regulations and best practices. Engaged in regulatory compliance with EHS and IACUC protocol adherence through comprehensive inspections. Responsible for overall lab safety.
- Mentorship and Training: Mentored new Ph.D. students and trained them on techniques and equipment usage, promoting continuous learning.
Education
Certificate - Biotechnology
Saint Louis Community College
Florissant, MO05.2010
Masters of Science All But Thesis - Exercise Science
Boise State University
Boise, ID05.1995
Associate of Science - Medical Assistant
United States Air Force School of Medicine
Wichita Falls, TX10.1988
Master of Arts - K-12 Certification Art Education
Francis Marion University
Florence, SC05.1987
Masters of Art in Teaching (M.A.T.) - Art Education
University of North Carolina At Chapel Hill
Chapel Hill, NC08.1984
Bachelor of Arts - Studio Arts And Minor Art History
Connecticut College
New London, CT12.1982
Skills
- Cell Culture
- Aseptic Technique
- Molecular Calculations (Solution preparation/ Molarity
- Flow Cytometry
- Microscopy/Confocal/TEM/SEM
- Technical Writing, Editing, and SOP development
- Microsoft products
- REDCap/ Workday/ WordPress
- Adobe photoshop/ illustrator/ In Design
- Laboratory Management
- Supplies, Ordering, and Inventory
- Environmental Health Safety (EHS)
- Hazardous Waste Management
- Mouse Colony management and husbandry
- Mouse Echocardiograms
- IP and Subcutaneous injections
- IACUC & IRB
- Scientific Presentations
- Ethics and integrity
- Documentation And Reporting
- Data Analysis
- Data Visualization/ Power BI
- Curriculum Development
- Computer Skills
- Teaching Experience
- Customer Relationship Management
- Project Development & Implementation
- Budget Analysis
- Work Planning and Prioritization
Timeline
Senior Research Associate
Florida Stroke Registry/ University Of Miami
10.2022 - Current
Research Biologist
Bruce W. Carter VA Medical Center
07.2022 - 10.2022
Research Associate 2/Lab Manager
Ohio State University: Nuo Sun Lab
08.2018 - 07.2022
Research Associate 1
Ohio State University: CMIF
11.2017 - 08.2018
Technical Customer Service Representative
LabCorp
01.2016 - 08.2017
Receiving Technician
Mérieux NutriSciences
08.2015 - 01.2016
Research Associate 2
Ohio State University: Department of Radiation and Oncology/Department of Molecular Virology, Immunology, and Medical Genetics
03.2013 - 08.2015
Research Technician II
University of Pittsburgh: Department of Pharmacology and Chemical Biology
06.2010 - 03.2013
Certificate - Biotechnology
Saint Louis Community College
Masters of Science All But Thesis - Exercise Science
Boise State University
Associate of Science - Medical Assistant
United States Air Force School of Medicine
Master of Arts - K-12 Certification Art Education
Francis Marion University
Masters of Art in Teaching (M.A.T.) - Art Education
University of North Carolina At Chapel Hill
Bachelor of Arts - Studio Arts And Minor Art History
Connecticut College
Work Availability
monday
tuesday
wednesday
thursday
friday
saturday
sunday
morning
afternoon
evening
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Quote
Success demands resilience. Stay tough, hold your ground, and fight relentlessly for your dreams. Victories are won by those who refuse to give up.
Dr. Martin Ross Krigman
Publications
- Pharmacological inhibition of USP30 activates tissue-specific mitophagy, Luo, Hongke, Krigman, Judith, Zhang, Ruohan, Yang, Mingchong, Sun, Nuo, Acta Physiologica, 04/23/2021, https://doi.org/10.1111/apha.13666
- State of the Florida Stroke Coordinator: Hospital Inventory Survey Insights, Sessa, J, Gutierrez, CM, Gardener, H, Krigman, JD, Ampie, C, Hylton, T, Sobczak, V, Rundek, T, Romano, JG, Gordon-Perue, GL, 02/2024
- Ten-Year Review of Antihypertensive Prescribing Practices After Stroke and the Associated Disparities From the Florida Stroke Registry, Gordon-Perue, GL, Ying, H, Bustillo, A, Zhou, L, Gutierrez, CM, Gardener, HE, Krigman, JD, Jameson, A, Dong,C, Rundek, T, Rose, DZ, Romano, JG, Alkhachroum, A, Sacco, RL, Asdaghi, N, 12/2023
- A 10-year review of antihypertensive prescribing practices after stroke and the associated disparities from the Florida Stroke Registry, Gordon-Perue, GL, Ying, H, Bustillo, A, Zhou, L, Gutierrez, CM, Wang, K, Gardener, HE, Krigman, JD, Jameson, A, Foster, D, Dong,C, Rundek, T, Rose, DZ, Romano, JG, Alkhachroum, A, Sacco, RL, Asdaghi, N, 02/2023
- Significance of mitochondrial activity in neurogenesis and neurodegenerative diseases, Ozgen, S, Krigman, JD, Zhang , R, Sun, N, Neural Regen Research, 08/2021
- Assessment of mitophagy in human iPSC-derived cardiomyocytes, Yang, M, Fu, FD, Zou, J, Sridharan, D, Zhao, MT, Singh, H, Krigman, JD, Khan, M, Xin, G, Sun, N, 02/2022
- The Mitochondrial Deubiquitinase USP30 Regulates AKT/mTOR Signaling, Zhang, R, Ozgen, S, Luo, H, Krigman, JD, Xin, G, Zhao, Y, Sun, N, 02/2022
- Pharmacological Inhibition of USP30 activates Tissue-specific Mitophagy, Luo, H, Krigman, JD, Zhang, R, Yang, M, Sun, N, 04/2021
- A Healthy Heart, and a Healthy Brain: Looking at Mitophagy, Luo H, Zhang R, Krigman JD, McAdams A, Ozgen S, Sun N, Front. Cell Dev. Biol., 05/2020
- Mitophagy in Cardiovascular Homeostasis, Zhang, R, Krigman, JD, Hongke, L, Ozgen,S, Yang, M, Nuo, N, 04/2020
- Detection of mitophagy in mammalian cells, mice, and yeast, Calvellia H, Krigman J, Onishid M, Narendraa D, Sun N, Okamotod K, Methods in Cell Biology, 01/2019
References
- Mahmood Khan, Associate Professor, Department of Emergency Medicine, Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, Room 405C, 473 West 12th Avenue, Columbus, Ohio, 43210, 614-688-7803, mahmood.khan@osumc.edu
- Brian Kemmenoe, Senior Microscopist, 245BRT, 460 12th Avenue, Columbus, Ohio, 43231, 614-292-7406/740-366-3929, Kemmenoe.1@osu.edu
- Penny Jones, Department Administrator: Physiology and Cell Biology, Davis Heart and Lung, Room 110 Davis Heart and Lung, Columbus, Ohio, 43210, 614-685-9175, Penny.jones@osumc.edu
- Kathy Bondra, Research Associate, UT Health San Antonio, Greeley Children's Cancer Research Institute, 1558 Enchanted River Drive, Bandera, Texas, 78003, 614-572-5505, kmbondra@yahoo.com
- Britt Eubanks, IT/Storage/Disaster Recovery Specialist, International Paper, 2118 Golfway Drive, St Charles, Missouri, 63301, 314-574-6928, Britt.eubanks@gmail.com
- Luis Gonzalez, MHA, Administrative Officer, Research and Development, Miami VA Healthcare System (546/151), 305-575-3179, Luis.Gonzalez2@va.gov
- Nuo Sun, Assistant Professor: Department of Physiology and Cell Biology, Davis Heart and Lung, Room 410 Davis Heart and Lung, Columbus, Ohio, 43210, 614-685-0023, Nuo.Sun@osumc.edu
- Julie Dougherty, Research Scientist | Dr. William Smoyer Lab, Center for Clinical & Translational Research | Division of Nephrology & Hypertension, Abigail Wexner Research Institute, RB1 3rd floor, Columbus, Ohio, 43205, 440-670-0880, Julie.Dougherty@nationwidechildrens.org
- Jose Romano, M.D., Professor of Neurology, University of Miami Miller School of Medicine, 1120 NW 14th Street, Miami, Florida, 33136, jromano@med.miami.edu
- Gillian Gordon Perue MBBS, DM, Chief of Neurology and Stroke - Jackson South Hospital, University of Miami Miller School of Medicine, 1120 NW 14th St, 13th floor, Miami, Florida, 33136, ggordonperue@miami.edu
- Carolina M. Gutierrez, PhD, Research Assistant Professor, Stroke Division, University of Miami Miller School of Medicine, 1120 NW 14th Street, Suite 1370, Miami, Florida, 33136, 305-243-7850, cgutierrez2@med.miami.edu
digital painting
Experienced digital artist, realism, influenced by 16th, 18th century painters. Enjoy whimsical subjects with a realistic twist. Typically use Krita software, but exploring Photoshop, Adobe Illustrator, and InDesign.
My profile background is part of an illustration I did for a recent book-
Publications
- Pharmacological inhibition of USP30 activates tissue-specific mitophagy, Luo, Hongke, Krigman, Judith, Zhang, Ruohan, Yang, Mingchong, Sun, Nuo, Acta Physiologica, 04/23/2021, https://doi.org/10.1111/apha.13666
- State of the Florida Stroke Coordinator: Hospital Inventory Survey Insights, Sessa, J, Gutierrez, CM, Gardener, H, Krigman, JD, Ampie, C, Hylton, T, Sobczak, V, Rundek, T, Romano, JG, Gordon-Perue, GL, 02/2024
- Ten-Year Review of Antihypertensive Prescribing Practices After Stroke and the Associated Disparities From the Florida Stroke Registry, Gordon-Perue, GL, Ying, H, Bustillo, A, Zhou, L, Gutierrez, CM, Gardener, HE, Krigman, JD, Jameson, A, Dong,C, Rundek, T, Rose, DZ, Romano, JG, Alkhachroum, A, Sacco, RL, Asdaghi, N, 12/2023
- A 10-year review of antihypertensive prescribing practices after stroke and the associated disparities from the Florida Stroke Registry, Gordon-Perue, GL, Ying, H, Bustillo, A, Zhou, L, Gutierrez, CM, Wang, K, Gardener, HE, Krigman, JD, Jameson, A, Foster, D, Dong,C, Rundek, T, Rose, DZ, Romano, JG, Alkhachroum, A, Sacco, RL, Asdaghi, N, 02/2023
- Significance of mitochondrial activity in neurogenesis and neurodegenerative diseases, Ozgen, S, Krigman, JD, Zhang , R, Sun, N, Neural Regen Research, 08/2021
- Assessment of mitophagy in human iPSC-derived cardiomyocytes, Yang, M, Fu, FD, Zou, J, Sridharan, D, Zhao, MT, Singh, H, Krigman, JD, Khan, M, Xin, G, Sun, N, 02/2022
- The Mitochondrial Deubiquitinase USP30 Regulates AKT/mTOR Signaling, Zhang, R, Ozgen, S, Luo, H, Krigman, JD, Xin, G, Zhao, Y, Sun, N, 02/2022
- Pharmacological Inhibition of USP30 activates Tissue-specific Mitophagy, Luo, H, Krigman, JD, Zhang, R, Yang, M, Sun, N, 04/2021
- A Healthy Heart, and a Healthy Brain: Looking at Mitophagy, Luo H, Zhang R, Krigman JD, McAdams A, Ozgen S, Sun N, Front. Cell Dev. Biol., 05/2020
- Mitophagy in Cardiovascular Homeostasis, Zhang, R, Krigman, JD, Hongke, L, Ozgen,S, Yang, M, Nuo, N, 04/2020
- Detection of mitophagy in mammalian cells, mice, and yeast, Calvellia H, Krigman J, Onishid M, Narendraa D, Sun N, Okamotod K, Methods in Cell Biology, 01/2019
References
- Mahmood Khan, Associate Professor, Department of Emergency Medicine, Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, Room 405C, 473 West 12th Avenue, Columbus, Ohio, 43210, 614-688-7803, mahmood.khan@osumc.edu
- Brian Kemmenoe, Senior Microscopist, 245BRT, 460 12th Avenue, Columbus, Ohio, 43231, 614-292-7406/740-366-3929, Kemmenoe.1@osu.edu
- Penny Jones, Department Administrator: Physiology and Cell Biology, Davis Heart and Lung, Room 110 Davis Heart and Lung, Columbus, Ohio, 43210, 614-685-9175, Penny.jones@osumc.edu
- Kathy Bondra, Research Associate, UT Health San Antonio, Greeley Children's Cancer Research Institute, 1558 Enchanted River Drive, Bandera, Texas, 78003, 614-572-5505, kmbondra@yahoo.com
- Britt Eubanks, IT/Storage/Disaster Recovery Specialist, International Paper, 2118 Golfway Drive, St Charles, Missouri, 63301, 314-574-6928, Britt.eubanks@gmail.com
- Luis Gonzalez, MHA, Administrative Officer, Research and Development, Miami VA Healthcare System (546/151), 305-575-3179, Luis.Gonzalez2@va.gov
- Nuo Sun, Assistant Professor: Department of Physiology and Cell Biology, Davis Heart and Lung, Room 410 Davis Heart and Lung, Columbus, Ohio, 43210, 614-685-0023, Nuo.Sun@osumc.edu
- Julie Dougherty, Research Scientist | Dr. William Smoyer Lab, Center for Clinical & Translational Research | Division of Nephrology & Hypertension, Abigail Wexner Research Institute, RB1 3rd floor, Columbus, Ohio, 43205, 440-670-0880, Julie.Dougherty@nationwidechildrens.org
- Jose Romano, M.D., Professor of Neurology, University of Miami Miller School of Medicine, 1120 NW 14th Street, Miami, Florida, 33136, jromano@med.miami.edu
- Gillian Gordon Perue MBBS, DM, Chief of Neurology and Stroke - Jackson South Hospital, University of Miami Miller School of Medicine, 1120 NW 14th St, 13th floor, Miami, Florida, 33136, ggordonperue@miami.edu
digital painting
Experienced digital artist, realism, influenced by 16th, 18th century painters. Enjoy whimsical subjects with a realistic twist. Typically use Krita software, but exploring Photoshop, Adobe Illustrator, and InDesign
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