Thomas E.J. Noonan
Raleigh,NC
Summary
Accomplished Senior Research Associate at Tessera Therapeutics with expertise in gene editing and automation development. Proven track record in enhancing screening throughput by 300% and implementing high-throughput assays. Skilled in data analysis and process improvement, fostering collaboration and training within teams to drive successful project outcomes.
Overview
7
7
years of professional experience
Work History
Senior Research Associate
Tessera Therapeutics
Somerville, MA
05.2023 - 01.2026
- Experienced with experimental design and execution including RNA plate production, nucleofection, culture, and gDNA extraction of many cell types to produce reliable and reproducible gene editing results in vitro
- Cultured and edited immortalized and primary cells (T cells, hepatocytes, HSCs)
- Designed polypeptides, spacers, and template guide RNAs for gene editing experiments
- Lentiviral transfection of 293T HEK cells to create stable landing pad cell lines for new targets
- Significant experience handling in vivo samples (mouse/NHP) to isolate desired cells for sorting and analysis
- Developed and onboarded automation protocols tripling screening throughput
- Experienced using liquid handling, imaging and flow cytometry automation (Hamilton, Agilent, TECAN, Biomek, SONY Sorter, iQue3, Novocyte, Cellario, Formulatrix, Celigo)
Clinical Process Development Associate I - CLIA #22D2055652
Broad Institute of MIT and Harvard
Cambridge, MA
06.2020 - 08.2021
- Implemented and scaled new COVID-19 diagnostic platform from 100 tests/day to at least 100,000 tests/day
- Executed bulk RNA sequencing and 10X single cell plating, library construction, and Illumina NGS to support advanced genomic analysis
- Conducted high-throughput assays with 96 and 384 well formats using developed automation workflows to enhance testing capabilities
- Leveraged liquid handling automation tools, including Hamilton STAR, Agilent Bravos, and Tempest, to optimize laboratory workflows
Research Assistant
Brigham and Women's Hospital, Harvey-Cushing Laboratory
Boston, MA
06.2019 - 05.2020
- Executed in vivo (IM, SC dosing) and ex vivo oncological studies with necropsy and analysis
- Generated lentiviral and AAV vectors, creating stable transfection of cells to support gene therapy research
- Prepared qualitative analysis assays via gel electrophoresis, Western blots, Northern blots, qPCR, ELISA
- Conducted immunological and histological staining assays on various tissue types to aid in disease characterization
- Facilitated onboarding of six new lab members to ensure effective workflow
Education
Master of Engineering - Biomedical Engineering
BOSTON UNIVERSITY COLLEGE OF ENGINEERING
Boston, MA05.2023
Bachelor of Science - Biology Major, Chemistry and Neuroscience Minor
WAKE FOREST UNIVERSITY
Winston-Salem, NC05.2019
Skills
- GraphPad Prism
- Gene Editing
- High Throughput Screening
- Cell Sorting
- FlowJo
- MATLAB
- Benchling/Jira
- ELN Design
- Cell Sorting and Flow
- Guide RNA Design
- Process Improvement
- Automation Development
- qPCR
- Viral Transfection
- Cell Culture
- NGS
- Electroporation
- gDNA extractions
- Data Analysis
- SolidWorks
Projects
3D mapping ICD lead: Designed a removable sheath for ICD leads to visualize them without fluoroscopy. Experience with device design and manufacturing, ISO/EU MDR, V&V testing, documentation.
Timeline
Senior Research Associate
Tessera Therapeutics
05.2023 - 01.2026
Clinical Process Development Associate I - CLIA #22D2055652
Broad Institute of MIT and Harvard
06.2020 - 08.2021
Research Assistant
Brigham and Women's Hospital, Harvey-Cushing Laboratory
06.2019 - 05.2020
Master of Engineering - Biomedical Engineering
BOSTON UNIVERSITY COLLEGE OF ENGINEERING
Bachelor of Science - Biology Major, Chemistry and Neuroscience Minor
WAKE FOREST UNIVERSITY