Juliete Macauley
Germantown,MD
Summary
Results-driven Molecular Biology Technician with extensive hands-on expertise in DNA/RNA workflows, cell-based assays, and laboratory instrumentation. Proven experience as a GMP Coordinator enhances the ability to support compliant, audit-ready operations through meticulous documentation, comprehensive training, and stringent quality oversight. Demonstrates exceptional proficiency in rodent handling and in vivo study support, ensuring precise, ethical, and well-documented experimental execution in both research and regulated laboratory settings. Committed to advancing scientific inquiry while upholding the highest standards of integrity and compliance.
Overview
10
10
years of professional experience
3
3
Certification
Work History
Senior Research Associate/Lab Manager
Immunomic Therapeutics
01.2015 - 01.2026
- Generated antigen - specific ELISA for triple Negative breast cancer study. Data used for IND submission cleared by FDA
- Performed validation of various plasmids by transient transfection and Western Blots used for IND submissions cleared by FDA
- Performed Elispot to identify T-Cell immune Response for various Oncology and Allergy studies
- Generated nucleic acid potency number by flow cytometry
- Determined encapsulated LNP concentration by Quant Ribo Green Assay
- Validated mRNA integrity by Agarose gel and optimized protocol
- Performed Stability testing on DNA Drug products batch Records for different time periods.
- Performed BCA Assays, Miniprep, transfections, SDS-PAGE to support studies
- Optimized Western Blots for many studies and implemented the iBind western system for quick results
- Generated, reviewed and optimized SOPs
- Developed, passages, cryo preserved and maintained cell bank for 293T, BHK1 cell lines using Aseptic Techniques
- Inoculated mice SC or IM, ID, with various plasmids using needle free Biojector, performed animal husbandry and related animal studies
- Created lab equipment inventory, assigned asset tag and log books for various equipment
- Generated SOPs for monitoring and recording of controlled Temperature units and trained staff
- Coordinated equipment calibrations, certifications, maintenance and procurement
- Ensured proper documentation of experiments, results and protocols
- Negotiated with vendors and evaluating cost-effective solutions
- Evaluated new technologies, methods and equipment that could advance lab capabilities
- Conducted pre-audit inspections and kept lab audit ready
- Ordered and prepared reagents, organized storage room and lab
- Good knowledge of Federal Agency Regulations, OSHA/GLPs/GMPs/GDocPs
ORISE Fellow
Food and Drug Administration FDA/CBER
01.2008 - 01.2014
- Performed DNA extraction from mouse tail clipping, performed breeding, weaning, maintaining mouse colony, tattooing mice and updating mice records.
- Injected mice by IP/SQ with DNA or tumorigenic cell lines, weighed, monitored and reported health status of animals. Administered drugs IP, IV, oral gavage and topical.
- Assist in Pre/post- surgical care. Assist in testing DNA oncogenicity and tumorigenicity in different strains of mice, hamsters and rats.
- Performed cell culture and molecular biological techniques including: DNA/RNA isolation, gel electrophoresis, enzyme digestion, ELISA, viral titration, plaque assays, cell migration and invasion assays, PCR genotyping of mice, western blots, cloning, transfection.
- Animal technician experience including:
- Establishment of cell lines from mouse tissues/tumors Collect animal fluids and tissues, mouse postmortem, dissection, tumor measurement, p necropsy, handling and processing mouse tissues/tumors for histopathology
Lab Animal Technician
Priority One Services
01.2006 - 01.2008
- Performed various animal husbandry tasks such as feeding, cage change, animal room.
- Sanitized, sterilized and organized animal supplies, monitored animals for any health concerns, and reported to Investigators involved in experiment.
- Received and documented animals.
- Monitored room temperatures and air conditions in animal rooms and made suggestions to management to improve overall animal well-being.
- Administered treatments according to Vet’s orders and euthanized animals using proper euthanasia techniques.
Education
Pre-Nursing - Biology, Chemistry, Anatomy
2007
Bachelor of Science - International Business
01-2005
Skills
- Western Blots
- Transfection
- Elispot
- ELISA
- DNA/RNA Extraction
- RiboGreen/RNA quantification
- Agarose gel electrophoresis
- PCR/SDS Page
- Cell and Tissue culture
- Aseptic Technique
- Animal Technician
- Laboratory management
- Federal Regulations, OSHA, GLP,GMP, QA, GDocP
- Effective Communication
- Critical Thinking and Time management
Certification
- FOUNDATION FOR ADVANCE EDUCATION IS SCIENCE (FAES)
- Tools and Technology for quantification and functional Analysis in Mammalian Cells
- DNA Methodology
- AMERICAN ASSOCIATION FOR LABORATORY ANIMALS (AALAS)
- Assistant Laboratory Animal Technician (ALAT)
LANGUAGES
English: Fluent
French: Proficient
German: Basics
Posters and Publications
- Shen W, Macauley J,and Heiland T. (2024) A UNITE-based self-amplifying RNA vaccine advances anti-tumor immunity in a murine triple-negative breast cancer model. Poster presentation (The Society for Immunotherapy of Cancer (SITC) annual meeting, Houston
- Amit S Adhikari , Juliete Macauley , Yoshimi Johnson , Mike Connolly , Timothy Coleman , Teri Heiland. Development and Characterization of an HCMV Multi-Antigen Therapeutic Vaccine for Glioblastoma Using the UNITE Platform (2022), Front.Oncol., Volume 12 :2022
- Shen W, Macauley J, Wilson G, Heiland T. (2019) Glypican-3-preluminal-LAMP: a novel DNA vaccine for liver cancer. Poster presentation (The International Liver Cancer Association 2019 annual meeting, Chicago
- Shen W, Marketon A, Su Y, Macauley J, Heiland T. (2016) Development of LAMP-based vaccine for cashew allergy immunotherapy. Poster presentation (Immunology 2016 annual meeting, Seattle).
- Belete Teferedegne, Juliete Macauley, Gideon Foseh, Eugenia Dragunsky, Konstantin Chumakov, Haruhiko Murata, Keith Peden, Andrew M. Lewis Jr. MicroRNAs as Potential Biomarkers for VERO Cell Tumorigenicity (under Review).
- Lauren R Brinster, Romelda L Omeri, Gideon S Foseh, Juliete N Macauley, Philip J Snoy, Joel J Beren, BeleteTeferedegne, Keith Peden and Andrew M. Lewis Jr. (2013).Failure-to-Thrive Syndrome Associated with Tumor Formation by Madin-Darby Canine Kidney Cells in NewBornoce Mice. Comparative Medicine, 2013, 63:323-330
- Murata H, Macauley Juliete, Lewis Andrew Jr, Peden K. Plaque Purification as a Method to Mitigate the Risk of AdventitiousAgent Contamination in Influenza Vaccine Virus Seeds. Vaccine. 2011, 29:3155-3161.
- Teferedegne, B., Radke, J.J., Macauley,B.J., Wei, T., Foseh, G., Cook, J., and Lewis, A. Jr (2014). MIR-376a regulates tumorigenic VERO cell expression of MICB, a ligand of the natural killer cell receptor NKG2D. In preparation
Computer competencies
- Detailed record keeping with DoccoLabs, Sharepoint
- Microsoft Word, Excel,PowerPoint, GraphPad Prism, Kaluza, Flowjo for data analysis and presentation
- Outlook and Teams for Communication.
Equipment competencies
- IBind Western Blot system, Turbo Trans-Blot System, Gbox9 imager, Cytoflex, Little Lunatic, Necleocounter-200, Epoch Plate washer and Reader, Gel Electrophoresis, BioRad Gel Transfer system.
- Autoclaves, BSL-3, BSL-4 Hoods, fumed Hoods, cell culture incubators, Centrifuge.
- Microscopes
Timeline
Senior Research Associate/Lab Manager - Immunomic Therapeutics
01.2015 - 01.2026
ORISE Fellow - Food and Drug Administration FDA/CBER
01.2008 - 01.2014
Lab Animal Technician - Priority One Services
01.2006 - 01.2008
Prince Georges Community College - Pre-Nursing, Biology, Chemistry, Anatomy
Schiller International University - Bachelor of Science, International Business