Kate Mazur
Sandown,US
Summary
Diligent biotechnology master's student and graduate research assistant seeking to further her passion and knowledge for science through hands-on industry experience.
Overview
1
1
year of professional experience
Work History
Graduate Laboratory Research Assistant
University of New Hampshire
09.2023 - Current
- Conducted research surrounding culturing, maintenance, and microscopy of the PC12 cell line
- Utilized reflectance confocal, stimulated emission depletion (STED) and immunofluorescent microscopy for PC12 differentiation analysis
- Directed a separate independent research pathway for the laboratory
- Managed inventory of laboratory supplies, including media preparation and preparation of both 3D collagen hydrogels and polyacrylamide gels
- Provided mentorship and training for new laboratory research members
Education
Master of Science - Biotechnology: Industrial And Biomedical Sciences
University of New Hampshire
Manchester, NH05.2025
Bachelor of Science - Biomedical Sciences: Medical Laboratory Sciences
University of New Hampshire
Durham, NH05.2024
Skills
- Proficiency in culturing and maintenance of various cell lines
- Microscopy technical experience with reflectance confocal, brightfield, and fluorescent microscopes
- Immunofluorescent staining and immunofluorescent microscopy experience
- Expertise in both collagen gel and polyacrylamide formation
- Proficiency in lab techniques such as flow cytometry, viability and cytotoxicity assays, and Resazurin assays
- Hazardous waste disposal and chemical handling
- Scientific writing experience
Projects
Differentiation of PC12 and NIH/3T3.2 Adherent Cell Lines in 3D Collagen Hydrogels:
- Prepared successful and adherent 3D collagen hydrogels that contained fluorescent polystyrene microbeads
- Seeded and cultured both PC12 and NIH/3T3.2 cell lines in the 3D collagen hydrogels
- Visualized the 3D collagen hydrogel samples in both reflectance confocal and STED microscopy
PC12 and NIH/3T3.2 Cells on Polyacrylamide Gels to Measure Traction Force Microscopy (TFM):
- Formulated polyacrylamide gels coated with either laminin or collagen-I and seeded both NIH/3T3.2 and PC12 cells on the polyacrylamide gels
- Analyzed the preliminary TFM approach through confocal microscopy
Prolonged Exposure of Pure Caffeine on Resulting PC12 Neurogenic Characteristics:
- Managed an individual study of the effects of prolonged caffeine exposure on PC12 neurogenic characteristics
- Treated PC12 cells in chamber slides with pure caffeine, nerve growth factor, or a combination of the two for one week
- Immunostained the PC12 cells with the monoclonal antibodies beta-3 tubulin and nestin and counterstained with actin red and DAPI
- Visualized the PC12 cells with immunofluorescent microscopy
Timeline
Graduate Laboratory Research Assistant
University of New Hampshire
09.2023 - Current
Master of Science - Biotechnology: Industrial And Biomedical Sciences
University of New Hampshire
Bachelor of Science - Biomedical Sciences: Medical Laboratory Sciences
University of New Hampshire
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