Kazutaka Fukumura

A large-scale genomic characterization of germline and somatic alterations in gliomas (a lead member for massive dataset generation)

• Managed over 5,000 clinical specimens for projects on gliomas
• DNA extraction from massive clinical specimens
• Carried out whole-exome sequencing and SNP array

Dissecting radiotherapy-induced immunogenicity in brain metastasis (a project leader)

• Managed over 400 clinical specimens of brain metastasis
• DNA and RNA extraction from FFPE materials
• Carried out whole-exome sequencing, RNA-sequencing and
  TCR-sequencing
• Integration and analysis of multiomic datasets

Characterization of spatial genome heterogeneity in diffuse gliomas (a project leader)

• DNA extraction from frozen glioma and blood specimens.
• Next-generation sequencing library preparation for whole-exome sequencing
• Methylation array
• Data analysis

Genomic characterization of familial gliomas (a lead member for data generation)

• DNA extraction from FFPE and blood materials
• Next-generation sequencing library preparation for whole-exome sequencing
• Methylation array

Multiomic characterization of brain metastasis (a project leader)

• DNA, RNA, and protein extraction from frozen brain metastasis tissue and blood samples
• Carried out whole-exome sequencing, RNA-sequencing and Reverse Phase Protein Array
• Immunofluorescence targeting immune cells
• Drug evaluation for brain metastasis mouse models and cell lines
• Mitochondrial analysis
• In vivo and ex vivo tumor burden evaluation with bioluminescence