Kristina Thanasi

Work in a histology laboratory conducting multi-step multiplex spatial genomics experiments.

• Conduct hands-on multi-step experiments involving RNA/DNA extraction, Sample Quality Control (QC), cDNA beads purification, library preparation, and Next-Generation Sequencing (NGS) to support spatial genomics and multi-omics research, aligning with cutting-edge technologies like Stereo-seq Spatial Transcriptomics.
• Collaborate with cross-functional teams, including scientists and assay developers, to optimize experimental workflows and contribute to new assay development projects.
• Play a key role in developing and validating innovative laboratory protocols for tissue sample processing, DNA/RNA purification, and sequencing, helping to refine research strategies in genomics .
• Maintain meticulous sample tracking across all stages, from quality control to analysis, ensuring data integrity and reproducibility for research projects.
• Contribute valuable feedback on experimental procedures, actively participating in process improvements and protocol development.
• Improved laboratory operations by 25% through efficient inventory management, streamlined equipment maintenance, enhanced safety protocols, and optimized space planning, ensuring smooth lab function and more effective use of resources
• Lead training initiatives for new team members, including designing training materials and developing leadership skills in laboratory settings.
• Present research findings in team meetings, collaborating with colleagues to align goals and drive project success.

• Maintained strict aseptic fields when collecting biological samples, significantly reducing the risk of staff and patient infections.
• Labeled transfer specimen tubes accurately and adhered to precise directions for handling and storing specimens for safe transport. Proficiently centrifuged blood samples following clinical protocols.
• Identified and resolved unexpected results, directing secondary testing measures or collection efforts, ensuring the accuracy and reliability of diagnostic data.

• Thawing cryopreserved cells, seeding them in culture vessels, maintaining optimal growth conditions, passaging to prevent over-confluence, and assessing cell viability and density using hemocytometry and trypan blue staining.
• Performed RT-PCR tests with precision, contributing to the timely and accurate analysis of nucleic acid samples.
• Prepared buffers for various laboratory techniques, enabling optimal conditions for experiments and analyses.
• Proficiently prepared media a for laboratory use, guaranteeing that the appropriate growth conditions were maintained for microbial cultures and cell lines.

• Phlebotomy and Patient Interaction
• Sample Collection and Labeling
• Exam Room Maintenance
• Sample Handling and Analysis
• Aseptic Technique and Sterility

• Complete Blood Count (CBC) Analysis: Proficiency in analyzing blood cell counts, hemoglobin levels, and hematocrit.
• Blood Smear Preparation and Evaluation: Expertise in preparing and interpreting peripheral blood smears.
• Coagulation Testing: Experience with tests such as PT, APTT, and INR.
• Cell Morphology Identification: Ability to identify various types of blood cells and abnormalities.
• Phlebotomy Skills: Competence in drawing blood samples from donors.
• Blood Component Separation: Familiarity with techniques for separating blood into components like plasma, platelets, and red blood cells.