Summary
Overview
Work History
Education
Skills
Pupblications
Accomplishments
LANGUAGE
Volunteering
RELATED COURSEWORK
Timeline
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LEONIE PFISTERER

Basking Ridge,NJ

Summary

Internationally trained life science graduate with an M.Sc. in Life Science Research (U.S.) and a B.Sc. in Biology (Germany), with practical research experience in cell culture, PCR, CRISPR/Cas9, and extracellular vesicle analysis. Supported sample preparation, protein analysis, and data review under GMP practices while maintaining accurate documentation and sterile technique. Research background spans microbial pathways and cancer biology including a poster presentation on adipocyte behavior in the tumor microenvironment. Balanced strong academic performance with competitive Division II field hockey, building discipline, teamwork, and adaptability.

Overview

3
3
years of professional experience

Work History

Research Assistant

University of Marburg
Marburg, Germany
03.2026 - 07.2026
  • Contributed to research on the crosstalk between adipocytes, extracellular vesicles, and tumor biology within a translational cancer research group.
  • Cultured and differentiated adipocytes, maintained mammalian cell lines under sterile conditions to support experiments on adipocyte biology in tumor microenvironment
  • Isolated, characterized, and analyzed extracellular vesicles (EVs) to elucidate their role as mediators of intercellular communication in cancer
  • Applied a broad range of wet lab techniques including sample preparation, protein analysis, and biochemical assays to support ongoing projects
  • Presented poster "De-differentiation of adipocytes in tumor microenvironment modulates extracellular vesicle biogenesis machinery" at UCT Science Day, June 2026

Intern

ImmuChrom GmbH
Heppenheim
08.2025 - 09.2025
  • Gained hands-on experience with HPLC and learned to become highly proficient in performing ELISA assays for routine analyses
  • Conducted data review and analysis to support quality control processes within the guidelines of GMP practices
  • Assisted with administrative and office tasks, including preparing 10-15 delivery notes and invoices per day

Student Researcher

Lincoln Memorial University
Harrogate, TN
08.2024 - 05.2025
  • Interpreted and analyzed Western blot data to confirm protein elimination
  • Used ImageJ and Microsoft Excel to prepare and manipulate extensive databases
  • Designed customized gene-editing experiments using CRISPR/Cas9 technology on rhabdomyosarcoma cells, enabling precise manipulation of the gene CNTRL

Graduate Assistant

Carnegie-Vincent Library
Harrogate, TN
08.2024 - 05.2025
  • Assisted over 10 patrons daily with technology access at the library. with use and access to technology, resources, and materials
  • Improved library organization by accurately shelving books and other materials according to the Library of Congress Classification system
  • Answered general questions in person, by phone, and over email

Tutor

Schuelerhilfe
Weyhe
01.2023 - 04.2023
  • Collaborated with groups of 5+ students to complete homework assignments, identify lagging skills, and correct weaknesses
  • Created tailored lesson plans which addressed individual learning styles for 10-20 students and increased average assignment grade by 1.2 points within the first 2 months
  • Tutored diverse groups of students to strengthen subject comprehension, boost confidence, and build important learning skills

Education

M.Sc. - Life Science Research

Lincoln Memorial University
Harrogate, TN
05-2025

B.Sc. - Biology

Carl-von-Ossietzky University Oldenburg
Oldenburg, Germany
08-2023

High School Diploma -

KGS Leeste
Leeste, Germany
06-2019

Skills

Molecular & Wet-Lab Techniques:

  • PCR, CRISPR-Cas9, SDS-PAGE, Western Blot, Protein purification, Immunohistochemistry, Fluorescent Microscopy, Cell Culture, Anaerobic Medium Preparation, DNA/RNA extraction

Scientific & Administrative:

  • Research Report Writing, Protocol Development, Literature Review, Scientific Methodology, Data Collection & Laboratory Notebook Management

Computational & Technical:

  • R/R Studio, MATLAB, Adobe Illustrator, ImageJ, Microsoft Office (Word, Excel, PowerPoint)

Soft Skills:

  • Teamwork, Prioritization Techniques, Organization, Time Management, Attention to detail to detail scheduling, Communication, Independent Work

Pupblications

  • Pfisterer, L., Boyle, C., Cole, A., et al. (2026)
    Disruption of the centriolin/Cep110 gene (CNTRL) with CRISPR/Cas9 leads to cell cycle arrest and cell death of rhabdomyosarcoma cells in vitro. Molecular Biology of the Cell, 37(2).
    https://doi.org/10.1091/mbc.E25-08-0365

Accomplishments

  • Student-Athlete as part of the womens Field hockey team at LMU and Team Leader
  • Academic Before Everything (A.B.E.) Award for academic excellence as a student-athlete at LMU in Fall 2023
  • Poster Presentation of Disruption of the centrosome protein Centriolin inhibits rhabdomyosarcoma cell proliferation at the 134th annual meeting of the Tennessee Academy of Science
  • Poster Presentation on De-differentiation of adipocytes in the tumor microenvironment modulates the extracellular vesicle biogenesis machinery at the UCT Science Day in June 2026

LANGUAGE

German Native Fluency, English Advanced Proficiency (104 TOEFL)

Volunteering

Red Cross, Volunteer, Weyhe, Germany, 07/2019 to 07/202

  • Supported engaging, fun, and smooth-running events by helping with organization and planning for over 10 classroom activities
  • Used strong interpersonal communication skills to convey information to parents, Boosted children's social and emotional development through positive reinforcement and consistent encouragement

RELATED COURSEWORK

Microbiology, Neurobiology, Introduction to Biological Data Analysis in Matlab, Biochemistry, Molecular Biology, Physiology, Immunology, Molecular Genetics, Cellular Biology, Statistics for Biologists

Timeline

Research Assistant

University of Marburg
03.2026 - 07.2026

Intern

ImmuChrom GmbH
08.2025 - 09.2025

Student Researcher

Lincoln Memorial University
08.2024 - 05.2025

Graduate Assistant

Carnegie-Vincent Library
08.2024 - 05.2025

Tutor

Schuelerhilfe
01.2023 - 04.2023

M.Sc. - Life Science Research

Lincoln Memorial University

B.Sc. - Biology

Carl-von-Ossietzky University Oldenburg

High School Diploma -

KGS Leeste
LEONIE PFISTERER