Molecular Biology:
· Molecular cloning (Golden Gate, Gibbson, REase); RNAi/shRNA, miRNA
· CRISPR technology, including CRISPR Knock-Out; Knock-In; CRISPR Base Editing, Prime Editing; and CRISPR RNA techniques
· In Vitro transcription and radioactive isotope experiments (32P)
· Protein purification techniques, including baculovirus, and bacterial expression system.
Cell Biology and Cancer Biology:
· Flow cytometry, confocal and fluorescent microscopy
· Stable cell line selection (Pool and single clones)
· DNA extraction, PCR, and Gel Electrophoresis
· RNA extraction and RT-PCR
· Western Blotting, Southern Blotting, Immunoprecipitation, ELISA, ChIP
Virology:
· Lentivirus vector development and virus package
· AAV vector development and Virus package
· Baculovirus system for protein purification
Management skills:
· Management of multiple product pipelines
· Management of small to medium-sized research groups (3-10 members)
· Effective management of time and priorities
Computer Science:
· Offices series (Word, Excel, Powerpoint, Outlook, Access)
· Adobe series (Photoshop, illustrator)
· VBA and Python