Summary
Accomplishments
Work History
Education
Skills
Techniques
Industrial product development
Peer reviewed publications
Languages
Work Preference
Interests
Timeline
Generic
Lipeng Wu

Lipeng Wu

Rockville,MD

Summary

Over 21 years of research experience in Molecular Biology, Cell Biology and Cancer Biology in both academic and industrial sectors.

Served as a product manager and R&D leader, specializing in CRISPR, cloning, lentivirus, and stable cell lines.

Skilled in product development and strategic management.

Accomplishments

· Achieved 15-20% annual revenue growth from 2021 to 2023.

· Developed and launched 10 products across various areas.

· Published 4 papers in the peer-reviewed journal Molecular Cell.

Work History

Senior Scientist & Product Manager

Origene Technologies Inc
01.2019
  • Manufacturing Management and R&D CRISPR Products and Research: CRISPR KN1.0; KN2.0, CRISPRa, CRISPRi, Lenti-CRISPR Knock-out, Knock-in; CRISPR Base Editing and Prime Editing Development of Lenti-Virus and AAV virus system; Stable cell line selection for CRISPR Knock-Out / Knock-In; Tet-On Expression system: All-in-one Tet-On Expression; Lenti-Tet-On Expression; Lenti-Tet-On shRNA;

CTRA fellow

National Institute of Health
01.2013 - 01.2019
  • Investigated the topic of DNA topology and its relationship with epigenetics
  • Expertise in specialized fields, including Radioactive isotope experiments and DNA topology skills.

Research Scientist

University of Michigan
01.2008 - 01.2013
  • Explored the function of MOF and MSL2 in leukemogenesis and the underlying mechanisms
  • Published 4 peer-reviewed papers in the distinguished journal Molecular Cell (Impact Factor 19.4).

Education

Ph.D. - Biochem And Molecular Biology

Peking University Health Science Center
Beijng, China
07.2008

Skills

Molecular Biology Techniques

Cell and Cancer Biology Techniques

Genomic Editing including CRISPR

Lentivirus and AAV development

Scientific Writing

Personal Blogger

Techniques

Molecular Biology:

· Molecular cloning (Golden Gate, Gibbson, REase); RNAi/shRNA, miRNA

· CRISPR technology, including CRISPR Knock-Out; Knock-In; CRISPR Base Editing, Prime Editing; and CRISPR RNA techniques

· In Vitro transcription and radioactive isotope experiments (32P)

· Protein purification techniques, including baculovirus, and bacterial expression system.

Cell Biology and Cancer Biology:

· Flow cytometry, confocal and fluorescent microscopy

· Stable cell line selection (Pool and single clones)

· DNA extraction, PCR, and Gel Electrophoresis

· RNA extraction and RT-PCR

· Western Blotting, Southern Blotting, Immunoprecipitation, ELISA, ChIP

Virology:

· Lentivirus vector development and virus package

· AAV vector development and Virus package

· Baculovirus system for protein purification

Management skills:

· Management of multiple product pipelines

· Management of small to medium-sized research groups (3-10 members)

· Effective management of time and priorities

Computer Science:

· Offices series (Word, Excel, Powerpoint, Outlook, Access)

· Adobe series (Photoshop, illustrator)

· VBA and Python

Industrial product development

  • All-in-one CRISPRa system
  • All-in-one CRISPRi system
  • All-in-one Lenti-CRISPR Knock-out system for stable cell line selection
  • All-in-one CRISPR RNA (CRSPRr) system
  • All-in-one Lenti-CRISPR RNA (CRSPRr) system
  • MSX/GS Selection System on HEK293T cells
  • Optimized In vitro RNA Transcription System
  • All-in-one Tet-On vector system with extremely low background noise
  • All-in-one Lenti-Tet-On-Expression (LTOE) system
  • All-in-one Lenti-Tet-On-shRNA (LTOR) system

Peer reviewed publications

  • H2B ubiquitylation promotes RNA Pol II processivity via PAF1 and pTEFb, Wu, L., Li, L., Zhou, B., Qin, Z., Dou, Y., Molecular cell, 54, 6, 920-31, 2014
  • ASH2L Regulates Ubiquitylation Signaling to H3 K4 methylation: transregulation of the MLL complex in higher eukaryotes, Wu, L., Lee, S., Zhou, B., Nguyen, U.T., Tan, S., Muir, T., Cierpicki, T., Dou, Y., Molecular cell, 49, 6, 1108-20, 2013
  • The RING finger protein MSL2 in the MOF complex is an E3 ubiquitin ligase for H2B K34 and is involved in crosstalk with H3 K4 and K79 methylation, Wu, L., Zee, B.M., Wang, Y., Garcia, B.A., Dou, Y., Molecular cell, 43, 1, 132-44, 2011
  • Two mammalian MOF complexes regulate transcription activation by distinct mechanisms, Li, X., Wu, L., Corsa, C.A., Kunkel, S., Dou, Y., Molecular Cell, 36, 290-301, 2009
  • Histone deacetylase inhibitor depsipeptide activates silenced genes through decreasing both CpG and H3K9 methylation on the promoter, Wu, L.P., Wang, X., Li, L., Zhao, Y., Lu, S., Yu, Y., Zhou, W., Liu, X., Yang, J., Zheng, Z., Molecular and cellular biology, 28, 3219-3235, 2008
  • The advancement of biological application and detection in the DNA methylation field, Wu, L., Zhu WG., Chinese Journal of Laboratory Medicine, 2004
  • Introduction of Epigenetics, Wu, L., Song, J., Zhu WG., Advanced Molecular and Cellular Biology, 2005

Languages

English
Full Professional
Chinese (Mandarin)
Native or Bilingual

Work Preference

Work Type

Full Time

Work Location

On-SiteRemoteHybrid

Important To Me

Career advancementCompany CulturePersonal development programs

Interests

Meditation

Reading

Running

Timeline

Senior Scientist & Product Manager

Origene Technologies Inc
01.2019

CTRA fellow

National Institute of Health
01.2013 - 01.2019

Research Scientist

University of Michigan
01.2008 - 01.2013

Ph.D. - Biochem And Molecular Biology

Peking University Health Science Center
Lipeng Wu