Overview
Work History
Education
Areas of Expertise
Timeline
Generic

Michael Vincent Pollante

San Diego,CA

Overview

7
7
years of professional experience

Work History

Scientist (Assay/Product Development)

Stemson Therapeutics
La Jolla
11.2023 - Current
  • Spoke as a panelist at the Oxford Global Organ Modeling & 3D Cell Culture Conference in London, UK, sharing insight to solutions on navigating the challenges of 3D cell culture model technologies
  • Incorporated cell-based assay approaches for in-process QC, providing 100% confidence in the ability to assess cell viability of Stemson's tissue engineered follicular units in-vitro prior to transplantations
  • Streamlined the workflow of tissue engineered construct fabrication by implementing new protocols, resulting in a 30% reduction in process time and achieving a 95% success rate in fabrication

Associate Scientist (Platform Development)

Stemson Therapeutics
La Jolla
02.2023 - 11.2023
  • Generated over 20 unique fluorescent and luciferase reporter lines for various cell types, enabling the tracking of individual cell contributions to hair follicle outcomes with 95% accuracy
  • Established a multi-target flow cytometry assay for a cutoff criteria of 99% triple positive for pluripotency to comprehensively profile over 10 wild type and 20 reporter iPSC lines
  • Cross-functionally collaborated on small molecule screenings of over 200 drugs, resulting in the identification of top-performing compounds to improve the cell identities for hair growth outcomes

Research Associate III (Reprograming/Engineering)

Fate Therapeutics
Poway, CA
11.2021 - 01.2023
  • Presented findings at 2022 Fate Annual Science Fair with positive reception from 500+ attendees & demonstrated 99% efficacy of Fate's iPSC Master Cell Bank platform for clinical applications
  • Optimized iPSC CRISPR engineering protocol which increased targeted mutations 2X-fold enabling faster experiments and accelerating research productivity
  • Developed proof of concept data for multi-loci targeted editing to demonstrate the feasibility of advancing Fate's iPSC-derived NK and T cell product candidates, resulting in a 90% success rate in R&D studies

Scientist I (Custom Cell Line Engineering)

Thermo Fisher Scientific Inc.
Carlsbad, CA
09.2019 - 11.2021
  • Established successful partnerships with 20 collaborators, leading to the successful completion of 15 large-scale and top level projects generating $6M in profits
  • Devised a comprehensive system to expedite custom CRISPR IVT gRNA orders worth over $600K annually, reducing order turnaround time from 2 weeks to 1 week
  • Spearheaded CRISPR Tools Custom Services Team to facilitate gRNA primer design & synthesis for over 200 projects from universities & industry labs, driving up revenue to $1.2M in the first year

CIRM Intern (Lawrence S.B Goldstein Lab, UCSD)

California Institute for Regenerative Medicine
La Jolla, CA
08.2018 - 09.2019
  • Independently presented findings at 2019 International Society for Stem Cell Research Conference reaching over 3000 attendees
  • Received full-time internship in the Goldstein Lab (UCSD) at the Sanford Consortium for Regenerative Medicine with a $43,000 stipend to support research initiatives
  • Utilized hiPSC derived neurons to develop a novel model of Alzheimer's Disease, leading to the identification of a key finding involved in APOE-HDL trafficking

LCMS Service Technician

Precision Diagnostics
San Diego, CA
01.2017 - 12.2022
  • Resolved over 100 technical issues with LC-MS/MS instrumentation to ensure optimal performance, achieving 99.7% uptime rate of laboratory operations to fulfill 24-hour turnaround of results
  • Developed and validated laboratory waste sterilization and reuse method and recommendations for cost-effective implementation, yielding savings of $500k+ annually
  • Instructed 35+ clinical lab technicians in solvent formulation, sample preparation, liquid automation handling & LCMS chromatography reading techniques to improve accuracy of results

Education

High School Diploma -

Lincoln High School
San Diego, CA
06-2013

Areas of Expertise

Cell Culture: Mammalian cell culture (iPSCs, Primary), 2D/3D cell culture, cell counting, thawing, passaging, cryopreservation, cell viability and cytotoxicity assays, tissue engineering

Gene Editing: CRISPR gene editing (KO, KI, SNP, multi-loci), CRISPR gRNA design and synthesis, PCR primer design, lentiviral transduction, transient adenoviral reprogramming

Characterization: Multi-color flow cytometry, FACS sorting, Western Blot, ELISA,  immunocytochemistry (ICC), sequencing (NGS, Sanger), PCR (RT-PCR, qPCR, ddPCR), gel electrophoresis

Software/Instrumentation: FlowJo, VectorNTI, Intergrative Genomics Viewer, ImageJ, Prism, IonTorrent, LC-MS/MS (Agilent, Sciex, Shimadzu, Thermo), Attune, Cytoflex, Novocyte, Cytation 5, Neo2, Gen 5

Timeline

Scientist (Assay/Product Development)

Stemson Therapeutics
11.2023 - Current

Associate Scientist (Platform Development)

Stemson Therapeutics
02.2023 - 11.2023

Research Associate III (Reprograming/Engineering)

Fate Therapeutics
11.2021 - 01.2023

Scientist I (Custom Cell Line Engineering)

Thermo Fisher Scientific Inc.
09.2019 - 11.2021

CIRM Intern (Lawrence S.B Goldstein Lab, UCSD)

California Institute for Regenerative Medicine
08.2018 - 09.2019

LCMS Service Technician

Precision Diagnostics
01.2017 - 12.2022

High School Diploma -

Lincoln High School

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Michael Vincent Pollante