Mohammad Shafiq

1. Efficiently manage the project and able to complete in limited resources and time. Completed several projects during my research which are evidenced by the published 11 research articles. 2. Creative and problem- solver, I have established many protocols and troubleshooting which are routinely used in the lab and involved in training of new students. 3. Good team player, I have worked in collaboration with different research groups within the institute as well as in other institutes that. I also train the new students coming in the lab.

Driven Postdoctoral Research Associate in [Area of expertise] with experience at [Type] institution. Detail-oriented and systematic with strong desire to develop abilities useful in [Type] employment.

• Gene Editing and Molecular Biology, Proficient in CRISPR-Cas9 for precise gene editing and genetic manipulation., Expertise in molecular cloning, Western blotting, RNA isolation, electrophoresis, and quantitative PCR (qPCR) for gene expression analysis.
• Cell Biology, Skilled in flow cytometry for cell sorting and analysis., Experienced in using Seahorse flux analyzer for metabolic profiling., Proficient in immunocytochemistry for cellular localization of proteins.
• Biochemistry, Expertise in spectrophotometry, fluorimetry, and enzyme assays for biochemical analysis., Experienced in ROS estimation to assess oxidative stress levels.
• Microscopy, Proficient in differential interference contrast microscopy, fluorescence microscopy, and confocal microscopy for high-resolution imaging.
• Animal Cell Culture, Skilled in maintaining transformed and primary cell cultures (Cardiomyocyte) for experimental studies.
• Histopathology, Expertise in immunohistochemistry, H&E staining, Picrosirius Red, WGA, Masson trichrome, and DAB staining for tissue analysis.
• In Vitro Screening, Conducted in vitro screening of curcumin derivatives synthesized by CSIR-CDRI for anticancer activity.
• Animal Models and Physiology, Developed animal models of pulmonary hypertension using subcutaneous administration of monocrotaline in rats and mice., Proficient in non-invasive blood pressure measurement using the tail-cuff method (Columbus Instruments)., Experienced in invasive blood pressure measurement via carotid artery cannulation (ADInstruments)., Skilled in right ventricle catheterization for measuring right ventricle pressure in rats and mice., Assessed cardiac hypertrophy using 2-D echocardiography (VisualSonics Fujifilm).
• Endothelial Function, Isolated pulmonary artery and aorta to assess pulmonary and aortic endothelial function using myograph (AD-Instrument) and isolated organ bath system (Charles-River Laboratories).
• Herbal Extracts and Nanoparticles, Evaluated the therapeutic efficacy of herbal extracts in spontaneously hypertensive rats (SHR)., Synthesized selenium-heparin nanoparticles and characterized them using dynamic light scattering (DLS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM).
• Biochemical Estimation, Conducted assays for MDA, GSH, ROS, and nitrite to evaluate oxidative stress and biochemical parameters.
• Animal Handling and Dose Administration, Experienced in handling various animal models including rats, mice, hamsters, guinea pigs, and rabbits., Proficient in multiple dose administration methods: per oral (p.o.), intravenous (IV), intraperitoneal (IP), intramuscular (IM), intraduodenal (ID), and subcutaneous (s.c.).

• Vascular Discovery: From Genes to Medicine - Scientific Sessions 2024 American Heart Association, Chicago, IL, 05/15/24 to 05/18/24
• American Heart Association's Scientific Session 2024, Chicago, IL, 11/16/24 to 11/18/24
• Activation of Endothelial GCN2 Kinase Contributes to Pulmonary Vascular Remodeling and Pulmonary Arterial Hypertension, Maggie Mengqi Zhu, Mohammad Shafiq, Jingbo Dai, Yi Peng, You-Yang Zhao, Newport Harbor Island Resort, Newport, RI, United States, 10/15/23 to 10/19/23
• Inhibition of MAP Kinase-Activated Protein Kinase 2 (MK2) Prevents Development of Pulmonary Hypertension, Shafiq M, Hanif K., Lima, Peru, 01/24/20 to 01/26/20
• Nutraceutical from Microbial Origin, Pyrroloquinoline Quinone (PQQ), Decreases Right Ventricular Pressure and Hypertrophy in Rat Model of Pulmonary Hypertension, Shafiq M, Kashif Hanif, 10th National Leh Symposium, CSIR-Institute of Genomics and Integrative Biology, 11/15/19
• Inflammation in Pulmonary Hypertension: A Study on the Involvement of p38MAPK/MAP Kinase-Activated Protein Kinase 2 Pathway, Shafiq M, Kashif Hanif, International Conference on 'Translational Research in Cardiovascular Science', Bengaluru, 02/15/19 to 02/17/19
• An Investigation on the Role of Mitogen-Activated Protein Kinase (MAPK)-Activated Protein Kinase 2 (MK2) in Pulmonary Hypertension, Shafiq M, Jagavelu K, Kashif Hanif, National Symposium on 'Molecular Medicine for Lifestyle Disorders,' CDRI, 11/17/17 to 11/18/17
• Optimization Techniques for Western Blotting, Immunohistochemistry (IHC), and Immunocytochemistry (ICC), CSIR-CDRI, Lucknow, 12/04/18
• 7th Annual Conference of the Society for Mitochondrial Research & Medicine, CSIR-CDRI, Lucknow, 11/28/18 to 11/30/18
• Optimization Techniques for Western Blotting, Immunohistochemistry (IHC), and Immunocytochemistry (ICC), CSIR-CDRI, Lucknow, 11/25/16
• Confocal Microscopy, CSIR-CDRI, Lucknow, 11/08/16 to 11/09/16
• Bio-entrepreneurship and Bio-enterprise Creation, Biotech Park, Lucknow, 09/16/16 to 09/17/16
• Scientific and Technical Awareness Program in Animal Experimentation, National Laboratory Animal Center (NLAC), CSIR-CDRI, Lucknow, 08/19/15 to 08/21/15
• Role of Biotechnology in Forensic Science, Department of Biotechnology/Microbiology, Adhunik Institute of Education and Research, Ghaziabad, 12/08/12
• Role of Bioinformatics in Biomedicine, Department of Biotechnology/Microbiology, Adhunik Institute of Education and Research, Ghaziabad, 03/29/12

• Incentive Award for Excellence in Research Publications, CSIR-Central Drug Research Institute, Lucknow, 2021
• Qualified National Level Junior Research Fellowship (JRF) Examination, Council of Scientific and Industrial Research (CSIR), India, 06/01/16
• Qualified National Level DBT-Junior Research Fellowship (DBT-JRF) Examination, Department of Biotechnology (DBT), Government of India, 2015
• Qualified National Level DBT-Junior Research Fellowship (DBT-JRF) Examination, Department of Biotechnology (DBT), Government of India, 2014
• Qualified Common University Entrance Test (CUET) for Ph.D., 2014
• Qualified Combined Entrance Exam for Biotechnology (CEEB), Jawaharlal Nehru University, New Delhi, 2012
• Qualified All India Entrance Exam (AIEE) for PG Program, Indian Council of Agricultural Research (ICAR), 2012

• European Society of Cardiology, 2023 - present
• Association for Molecular Pathology, 2023 - present
• Pulmonary Hypertension Association, 2023 - present
• American Thoracic Society, 2022 - present
• Canadian Cardiovascular Society, 2022 - present

• Postdoctoral Research Associate, Department of Pediatrics, Ann & Robert H. Lurie Children's Hospital of Chicago, 10/01/22, Present
• Senior Research Fellow, Department of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, India, 08/01/17, 06/30/21
• Junior Research Fellow, Department of Pharmacology, CSIR-Central Drug Research Institute, Lucknow, India, 08/01/15, 08/31/17
• Project Assistant, Department of Molecular and Structural Biology, CSIR-Central Drug Research Institute, Lucknow, India, 11/01/14, 08/31/15
• Master's Dissertation, Department Nanotherapeutics, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Lucknow, India, 01/01/14, 06/30/14

• Inhibition of Mitogen-Activated Protein Kinase (MAPK)-Activated Protein Kinase 2 (MK2) is Protective in Pulmonary Hypertension, Hypertension, 04/01/21, 10.1161/HYPERTENSIONAHA.120.15229
• Involvement of mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MK2) in endothelial dysfunction associated with pulmonary hypertension, Life Sci., 12/01/21, 10.1016/j.lfs.2021.120075
• Pyrroloquinoline quinone (PQQ) improves pulmonary hypertension by regulating mitochondrial and metabolic functions, PulmPharmacolTher, 08/24/22, 10.1016/j.pupt.2022.102156
• Inhibition of poly (ADP-ribose) Polymerase-1 (PARP-1) improves endothelial function in pulmonary hypertension, Pulm Pharmacol Ther, 06/01/23, 10.1016/j.pupt.2023.102200
• Cissus quadrangularis extract mitigates diabetic cardiomyopathy by inhibiting RAAS activation, inflammation and oxidative stress, Biomarkers, 08/03/22, 10.1080/1354750X.2022.2107703
• Antihypertensive Effect of a Novel Angiotensin II Receptor Blocker Fluorophenyl Benzimidazole: Contribution of cGMP, Voltage-dependent Calcium Channels, and BKCa Channels to Vasorelaxant Mechanisms, Front Pharmacol, 03/30/21, 10.3389/fphar.2021.611109
• Ethanolic extract of Cissus quadrangularis improves vasoreactivity by modulation of eNOS expression and oxidative stress in spontaneously hypertensive rats, Clin Exp Hypertens, 01/02/22, 10.1080/10641963.2021.1991942
• Naringin ameliorates type 2 diabetes mellitus-induced steatohepatitis by inhibiting RAGE/NF-κB mediated mitochondrial apoptosis, Life Sci., 09/15/20, 10.1016/j.lfs.2020.118118
• Involvement of fatty acid synthase in right ventricle dysfunction in pulmonary hypertension, Exp Cell Res, 10/15/19, 10.1016/j.yexcr.2019.111569
• Diethyl-4,4'-dihydroxy-8,3'-neolign-7,7'-dien-9,9'-dionate exhibits antihypertensive activity in rats through increase in intracellular cGMP level and blockade of calcium channels, Eur J Pharmacol, 03/15/17, 10.1016/j.ejphar.2017.01.044
• Discovery of monocarbonyl curcumin hybrids as a novel class of human DNA ligase I inhibitors: in silico design, synthesis and biology, RSC Adv., 2016
• Inhibition of MAP Kinase-Activated Protein Kinase 2 (MK2) Prevents Development of Pulmonary Hypertension, Pulm Circ, 03/26/21, 10.1177/2045894020976949
• Cellular and Molecular Mechanisms in Metabolic Disorders: Role of Inflammation and Oxidative Stress, Frontiers in Pharmacology