My journey in the medical profession is underpinned by a deep-rooted aspiration to become a physician This ambition is not solely about achieving a title, but a testament to my unwavering commitment to healthcare, patient welfare, and a profound understanding of human biology Every research endeavor I've undertaken fortifies this commitment, spotlighting my insatiable curiosity and dedication to the ever-evolving realm of health sciences Parallel to this, my entrepreneurial pursuits, particularly the inception of ROSHOPP, highlight a different facet of my personality This venture into the digital marketplace showcases my adaptability, business acumen, and a keen sense of market trends Moreover, my track record in mentorship and leadership, exemplified by awards from UMass Dartmouth, demonstrates my inherent ability to guide, uplift, and inspire my peers, emphasizing the importance I place on communal growth Above all, my diverse academic laurels from varied institutions underline my belief in lifelong learning, emphasizing a mindset that values continuous personal and professional development
NAOMI NOMMI
Research Associate/Lab Manager
Boston,MA.
Summary
Equipped with dual Bachelor of Science degrees in Biology and Biochemistry, I have carved a niche as a dynamic and accomplished research professional. With over four years of immersive laboratory experience, my expertise encompasses a wide spectrum of research methodologies, including advanced mammalian cell culture techniques and intricate molecular biology procedures. Specializing in the cultivation and management of diverse mammalian cell lines like HEK and murine, I have honed my skills in surgeries on mice, isolating cells from tissues, and developing protocols for cell line generation. My analytical prowess is further highlighted in deploying immunoassays, leveraging Western Blot for protein detection, and conducting ELISA-based high-throughput screening. Delving deeper into genetic analytics, I have mastered qPCR techniques, notably focusing on target genes such as p62, while ensuring method optimization.
Beyond the confines of the lab, my abilities shine in multi-color flow cytometry, comprehensive data analysis using software suites like R, GraphPad, and Excel, underpinning my holistic approach to research. My unwavering dedication to science is reflected in my meticulous attention to detail, robust problem-solving mindset, and the determination to advance the boundaries of knowledge. I pride myself on my adept communication skills, making it seamless to convey intricate results to diverse audiences and showcase critical thinking in experimental designs. Compassionate, punctual, and deeply rooted in mentorship, I am invested in nurturing the growth of junior colleagues, evidenced by my leadership roles and entrepreneurial ventures. As a freelancer, my versatility and adaptability in professional scenarios are apparent, and my commitment to accurate documentation, report writing, and team leadership further underscores my dedication to the field.
Overview
5
5
years of professional experience
3
3
Certifications
2
2
Languages
Work History
Research Associate
Brigham And Women's Pumonary Medicine
Boston, MA
05.2022 - 07.2023
Primary Investigator: Tesfaigzi Yohannes, PhD
2500 hours
- Performed mouse cigarette smoke exposure using CHTech smoke exposure equipment
- Performed mice surgery and airway instillation using SHP62: Oropharyngeal and intratracheal instillation
- Worked with murine models of lung disease relevant to molecular targets, including genotyping
- Detected specific proteins within cultured cells using Western Blot by collecting cells from tracheas, isolating them then isolating the proteins
- Utilized ELISA to detect a variety of inflammatory cells
- Following 6 months of exposure to smoke, mucus from mice was tested using qPCR (Thermo Fisher Taqman) to target genes, specifically p62
- The more mucus present, the more p62 was detected in the sample
- Optimized the methods used for PCR, qPCR and digestion using restriction enzymes
- Created, optimized, and standardized the immunofluorescence protocol used for immunocytochemistry and immunohistochemistry
- Assisted in performing multi-color flow cytometry (up to 10 colors) coupled with Flow jo to detect t-cells within samples
- Prior to performing flow cytometry, t-cells were isolated from blood samples and passaged
- Analyzed Flow cytometry data using GraphPad, Excel and R
- GraphPad and Excel were used to arrange data and R was primarily used to analyze multiple sets of data concurrently
- Performed surgery on mice to collect the tracheas so that cells were able to be isolated from the tracheas
- Once isolated, digestion was performed to remove the cells and then the primary cells were isolated
- The cells were passage from either 5cm or 3cm dishes depending on the size of the mouse the trachea was harvested from
- If larger dishes were used for smaller animal models, the cells would die
- Once the cell count reaches roughly 500,000 cells, they could be passaged to 10 cm and eventually to 15 cm which would then allow them to be stored in cryotubes within nitrogen tanks
- Wrote the entire protocol for stable cell line generation for mice cells as listed above
- Generated stable cell lines from HEK cells delivered to the lab by passaging them and freezing them so they could be used in in-vitro research
- Transfected cells with expression vectors, CRISPR Cas9 delivery in primary cells, and organoid cultures
- Conducted routine care for laboratory colonies of mice (30% time)
- Performed immunohistostaining of tissue samples
- Visualized tissue and cell morphology with fluorescent confocal microscopy
- Submitted and placed orders for supplies, chemicals, and other reagents
- Analyze data on R software and GraphPad.
Research Fellow
McLean Hospital / Harvard Medical School
Belmont, MA
06.2021 - 08.2021
Treatment and Etiology of Neuropsychiatric Disorders in Youth Laboratory. Center for Depression, Anxiety and Fear Research
Director: Kerry Ressler, MD/PhD
400 hours
● Characterized specific cell clusters proximate to the Amygdala, contributing to the broader understanding of neural pathways and connections.
● Expertly generated fresh frozen mouse brain sections utilizing a cryostat, ensuring the preservation of cellular structures and biochemical compounds.
● Employed advanced molecular techniques to effectively label distinct RNA molecules within brain tissue samples.
● Leveraged confocal microscopy to provide detailed visualization and analysis of tissue section morphology.
● Acquired practical experience and insights by shadowing experts during immunohistochemistry procedures, mouse surgical operations, and animal anesthesia administration.
Anesthesia Technician Intern
Beth Israel Lahey Hospital
Winchester, MA
06.2021 - 01.2022
240 hours
● Facilitated surgical procedures by efficiently providing and setting up essential disposable materials for shed blood collection. Depending on the surgical location, adeptly set up equipment for the delivery of salvaged and banked blood products, specifically utilizing Cell Saver and Rapid Infuser systems.
● Demonstrated strict adherence to sterile protocols by aseptically assembling autotransfusion disposables, ensuring patient safety and preventing contamination.
● Actively supported surgical teams by assisting in dressing and securely fastening IVs and Arterial lines, ensuring consistent and uninterrupted medication flow.
● Collaborated closely with Anesthesiologists, providing pivotal assistance during critical processes like intubation and extubation as directed, ensuring procedural precision and patient well-being.
● Maintained surgical suite readiness by diligently restocking all utilized supplies during surgeries, ensuring all equipment and materials were readily available for seamless surgical operations.
Teaching Assistant-STEM Courses
University Of Massachusetts Dartmouth
Dartmouth, MA
09.2020 - 01.2022
200 hours
- Partnered with teacher to plan and implement lessons following school's curriculum, goals, and objectives.
- Tutored struggling students individually and in small groups to reinforce learning concepts.
- Assisted teachers with classroom management and document coordination to maintain positive learning environment.
- Supported classroom activities, tutoring, and reviewing work.
- Worked with teacher to optimize learning plans based on student test grades and evaluations.
- Developed materials for classroom teachers to support instruction, lab work or in-class activities.
Certified Nurse Assistant
IntelyCare
Boston, MA
03.2018 - 01.2022
10,000 hours
● Diligently monitored and documented patient vital signs, ensuring timely interventions as required, and performed biometric assessments for blood glucose levels, ensuring optimal patient health.
● Demonstrated compassion and patient-centric care by offering grooming services to patients and actively engaging in genuine conversations, lending a listening ear to their stories during periods of downtime.
● Accompanied and advocated for patients during doctor appointments, ensuring they understood medical directions, and provided seamless continuity of care.
● Responded promptly to call bells, addressing patient needs and concerns with professionalism and a patient-first approach.
● Expertly assisted patients with their Activities of Daily Living (ADL), including toileting, bathing, and dressing, promoting patient independence and comfort.
● Efficiently collected and labeled various patient samples, such as blood, urine, stool, and sputum, facilitating accurate diagnostics and timely interventions.
Plant Research Assistant
University of Massachusetts Dartmouth
North Dartmouth, MA
10.2020 - 05.2022
700 hours
● Spearheaded research investigations into the nuanced foraging habits exhibited by different plant species in heterogeneous soil conditions, shedding light on inter-species interactions and ecological adaptations.
● Conducted detailed characterization of root responses to spatial variations in soil nutrient levels, providing insights into plant adaptability and soil-root dynamics.
● Employed advanced PCR-Q methodologies to accurately quantify specific genes within the root microbial community, aiding in the understanding of plant-microbial symbiotic relationships.
Research Assistant
University of Massachusetts Dartmouth
North Dartmouth, MA
09.2018 - 01.2022
300 hours
- Performed ELISA-based high throughput screening to detect antigens within large volumes of samples using both 96 and 386 well plates
- Performed Plasmid DNA isolation and DNA amplification using PCR
- Prepared Buffers prior to experiments using precise specifications/ measurements
- Determined DNA topology size using gel electrophoresis
- Separated proteins based on mass using SDS-PAGE
- Cloned human PIB gene into pET vector, and purified recombinant His-tag PIB gene using affinity chromatography
- Conducted environmental monitoring and performed Gram-positive and Gram-negative staining for the identification of bacteria
- Isolated pure cultures using aseptic techniques
- Performed bacterial endotoxin test method validation and monitoring and bioburden test method validation and monitoring
- Conducted ethylene oxide sterilization validation
- Utilized a variety of instruments and equipment including centrifuges, hemagglutination plates, inoculation loops, incubators, roux culture bottles and electron microscope.
Screener/ Technician
COVID, Professional Staffing Group
Boston, MA
07.2019 - 09.2021
- Emmerson College, MCPHS - 400 hours
- Impact Health: Women and Men Lacrosse championship
- Swap students and players for Covid testing
- Set-up testing reagent for COVID-19
- Use PCR (Abid) and Quidel methods to get COVID result.
Medical Secretary
Cross Country Staffing
West Roxbury, MA
07.2020 - 08.2020
- Registered new patients in electronic medical records prior to appointment scheduling.
- Maintained current and accurate medical records for patients.
- Documented patient medical information, case histories, and insurance details to facilitate smooth appointments and payment processing.
- Maintained office supplies inventory by checking stock, anticipating department needs, and placing and expediting orders.
- Answered telephone calls to offer office information, answer questions, and direct calls to staff.
- Enhanced office productivity by handling high volume of callers per day.
Learning Commons Assistant
Claire. T Library
North Dartmouth
09.2019 - 01.2020
- University of Massachusetts Dartmouth -200 hours
- Schedule study rooms for students
- Answer calls from students and other university
- Connect alumni and students to WIFI and help with technical issues.
Student Tutor
American Reads
North Dartmouth, MA
09.2018 - 01.2019
Total Hours Dedicated: 200
- Developing academic materials and lessons relevant to course work
- Help students understand course materials by using university resources
- Re-direct student concerns about course work to designated professor, University of Massachusetts Dartmouth
- Employed enthusiastic and caring approach to reach and motivate students.
- Teaching kids how to write, spell and pronounce
- Teaching the kids how to draw and count
Education
Bachelor of Science - Biology + Biochemistry
UMass Dartmouth
North Dartmouth, MASkills
Cell Biology Expertise: Proficient in mammalian cell culture techniques, adept at generating stable cell lines, and ensuring meticulous cell line maintenance, showcasing comprehensive cellular biology understanding
Additional Information
- AWARDS , Office of Undergraduate Research Award; UMass Dartmouth December 2021
- Won Second Place MMHRSP Final Presentation; Harvard July 2021
- Grant from Council on Undergraduate Research; News Wise June 2021
- Dean’s List; UMass Dartmouth 2018 - 2019
- Chancellors List; UMass Dartmouth 2019 – 2022
- Leadership and Mentorship Award; UMass Dartmouth May 2022
Accomplishments
Zeihun.N, Naomi.N, Yohannes.T (2023, May). Loss of B-cell interacting killer gene augments Cigarette smoke-induced lung inflammation and Emphysema in Mice. Paper sent for review to JCI Insight Journal
Zerihun.N, Jocelyn.R, Fhad.A, Naomi.N, Mizan.R, Yohannes.T (2023, March). B-cell modifying factor (Bmf) causes increased p62 levels to protect from cigarette smoke and allergen-induced inflammation. Paper sent for review to JCI Insight Journal
Naomi, Nommi, Tara Rajaniemi, Diana Barette, Chisom Unegbu, Emily Silva (2022, May). Root Response to
Microbial Community. Paper sent for review and will be presented at the Department of Biology and Other Conferences at UMass Dartmouth, North Dartmouth, MA.
Naomi, Nommi, Olga Ponomareva, Kerry Ressler. (2021, August 13). Characterization of Neurotransmitters Colocalized with Foxp2 in the Infralimbic Cortex and Amygdala. Poster presented at the McLean Mental Health Research Summer Program Symposium at McLean Hospital, Belmont, MA.
Naomi, Nommi, Olga Ponomareva, Kerry Ressler. (2021, October 19). Characterization of Neurotransmitters
Colocalized with Foxp2 in the Infralimbic Cortex and Amygdala. Poster presented at the Society for the Advancement of Chicanos/Hispanics and Native Americans in Science (SACNAS)
Naomi, Nommi, Olga Ponomareva, Kerry Ressler. (2021, October 22). Characterization of Neurotransmitters
Colocalized with Foxp2 in the Infralimbic Cortex and Amygdala. Poster presented at the Annual Biomedical Research Conference for Minority Students (ABRCMS)
Affiliations
- American Marketing Association
- The American society of cell Biology
- Society for Advancement of Chicanos/Hispanics & Native Americans in Science
- Annual Biomedical Research Conference for Minorities Scientists
Certification
Certified Nursing Assistant License with ARC/State July 2020-January 2025
Leadership
Committee for Student Involvement; UMass Dartmouth -400 Total hours Sept 2018- May 2022
President of Pre-Health Society (2021-2022) -200 hours
● Directed and curated an enriching speaker series, personally securing professionals and experts from various health domains to address and inspire our society's members.
● Held the key responsibility of making final decisions on essential club matters, such as approving the budget, overseeing advertisement strategies, and greenlighting office appointment proposals, ensuring the society's smooth and efficient operations.
● Fostered a collaborative environment by working closely with a dedicated team of board members, making collective decisions that impacted the club's direction, events, and strategies, reinforcing the society's influence and presence on campus.
Vice President of Biology Association (2020 - 2022) – 200 hours
● Played an instrumental role in orchestrating club activities, ensuring events were relevant, engaging, and beneficial for all members, fostering a vibrant community of biology enthusiasts.
● Spearheaded networking initiatives with other campus clubs, creating synergies, joint events, and shared resources that enhanced the overall student experience.
● Took the initiative to invite esteemed guest speakers, providing valuable insights into the ever-evolving world of biology, and penned influential proposals to the Student Senate, ensuring the club's objectives were well-aligned with the broader campus goals and regulations.
Interests
Singing, Yoga, Dancing
Outside of my rigorous academic and professional pursuits, I immerse myself in activities that fuel my soul and maintain my well-being. I have a deep-seated passion for singing, which offers me both a form of expression and a connection to various musical genres and rhythms. This passion extends to the realm of dance, where movement and rhythm allow me to express emotions and stories in a dynamic and energetic manner. Additionally, my practice of yoga serves as a testament to my commitment to mental clarity and physical health. These hobbies, combined, provide a harmonious balance to my life, ensuring I remain grounded while ambitiously chasing my dreams.
Volunteer
Donor Ambassador; American Red Cross; Boston, MA
July 2020- Present|100 hours
● Greeted and guided donors upon arrival, establishing an immediate rapport and ensuring they experienced a seamless and welcoming intake process.
● Oversaw the reception and hospitality zones, vigilantly upholding the organization's cleanliness and presentation standards to guarantee a pleasant and professional ambiance for all attendees.
● Took the initiative in managing donor communications, adeptly handling reminder calls and proactively rescheduling appointments to optimize donor availability and retention.
● Liaised between donors and medical staff, facilitating any necessary pre-donation medical checks or screenings.
● Demonstrated strong attention to detail by maintaining up-to-date records of donor interactions, ensuring accurate and timely data capture for the organization's database.
● Collaborated closely with the wider Red Cross team, contributing to strategy discussions and process improvement initiatives aimed at enhancing the overall donor experience.
Student Volunteer at Massachusetts Public Interest Research Group; Boston, MA
July 2020-2021|80 hours
● Engaged in phone banking, reaching out to potential supporters and disseminating crucial information.
● Spearheaded and organized events on campus, rallying student organizations to join vote coalitions and promote civic participation.
● Actively supported causes by signing petitions focused on alleviating hunger and combating homelessness in the community.
Peer Mentor for CAS 101 Biology students; North Dartmouth, MA
September 2021-May 2022|150 hours
● Conducted personalized one-on-one mentoring sessions, meeting with students weekly to ensure they grasped complex biological concepts and effectively addressed their academic concerns.
● Diligently documented each session by overseeing student check-ins and check-outs, ensuring a structured approach to mentoring.
● Compiled detailed report forms post-session, providing comprehensive summaries and insights into each student's understanding and areas of improvement.
● Assessed student’s needs and tailored mentorship sessions, crafting individual plans to offer targeted support.
● Continuously monitored and evaluated student progress, focusing on both short-term understanding and long-term academic goals.
● At the end of each semester, provided comprehensive write-ups summarizing the overall progress and achievements of each student.
Medical Observation
Medical Observation; Brigham and Women’s Hospital -ICU
Physician: Dr Elizabeth GayDecember 2022- Present|4 hours
● Procedural Observations: Attended various surgeries including cholecystectomies, spinal surgeries, and knee replacements, gaining insights into the intricacies of each procedure and the collaborative approach involved in patient care.
● Rounds Participation: Joined medical rounds with Dr. Gay and other physicians contributing to discussions, asking pertinent questions, and gaining a deeper understanding of patient care and treatment planning.
● Inquisitive Learning: Demonstrated a proactive approach in learning by asking insightful questions during procedures, and pursuing follow-up queries to clarify doubts and expand knowledge on surgical practices and patient management.
● Patient Interaction: Engaged with patients during consultations, ensuring their comfort, addressing their concerns, and observing the empathetic communication and rapport-building between physicians and patients.
● Documentation & Reflection: Diligently documented observations, insights, and learnings from each session, reflecting on the experiences to assimilate knowledge and identify areas for further inquiry and learning.
Virtual Pre-medicine Shadowing; Medical School Headquarters October 2020-September 2021|35 hours
● Diverse Medical Perspectives: Engaged in virtual sessions with seasoned professionals, including medical doctors, nurse practitioners, and gynecologists, gaining firsthand insights into their unique experiences, challenges, and triumphs in the medical field.
● Proactive Participation: Leveraged the opportunity to interact with healthcare experts by posing in-depth questions about the medical school journey. Topics included the intricacies of the MCAT process, effective study strategies, and actionable advice on enhancing my academic and extracurricular profile for medical school applications.
● Reinforced Learning: Demonstrated comprehension and retention of knowledge by successfully completing a rigorous 30-minute quiz at the end of each shadowing session, ensuring a deeper understanding of the discussed topics and continual self-assessment.
Medical Observation; Beth Israel Lahey Winchester Hospital -OR
Physician: Dr. Jude Telfort June 2021-August 2021|40 hours● Anesthesiology Immersion: Gained a deep understanding of anesthetic practices by closely shadowing several anesthesiologists during complex procedures, observing patient sedation, monitoring, and pain management techniques in real-time.
● Diverse Surgical Observations: Witnessed a multitude of surgical procedures, including cholecystectomies, spinal surgeries, and knee replacements, gaining insights into surgical techniques, team collaboration, and patient care dynamics.
● Engaged Learning Approach: Actively engaged during observations by asking pertinent questions to clarify and expand understanding, ensuring thorough documentation of insights and learnings in a dedicated notebook for future reference and reflection.
● Holistic Patient Consultations: Observed comprehensive patient consultations alongside multiple physicians, taking detailed notes on diagnostic, treatment planning, and communication processes to understand the holistic approach to patient care.
● Post-Operative Patient Care: Prioritized patient welfare by ensuring comfort during the critical post-operative recovery phase, providing necessary assistance, and offering refreshments such as snacks, water, and drinks to facilitate smoother recuperation.
Timeline
Research Associate
Brigham And Women's Pumonary Medicine
05.2022 - 07.2023
Research Fellow
McLean Hospital / Harvard Medical School
06.2021 - 08.2021
Anesthesia Technician Intern
Beth Israel Lahey Hospital
06.2021 - 01.2022
Plant Research Assistant
University of Massachusetts Dartmouth
10.2020 - 05.2022
Teaching Assistant-STEM Courses
University Of Massachusetts Dartmouth
09.2020 - 01.2022
Medical Secretary
Cross Country Staffing
07.2020 - 08.2020
Learning Commons Assistant
Claire. T Library
09.2019 - 01.2020
Screener/ Technician
COVID, Professional Staffing Group
07.2019 - 09.2021
Research Assistant
University of Massachusetts Dartmouth
09.2018 - 01.2022
Student Tutor
American Reads
09.2018 - 01.2019
Certified Nurse Assistant
IntelyCare
03.2018 - 01.2022
Bachelor of Science - Biology + Biochemistry
UMass Dartmouth
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