Na'Taja Hill

As a fellow, I worked for ORISE, serving as a microbiologist at the CDC. I was responsible for working with the Mumps virus by testing the numerous genotypes (A, C, H, K, and D). The different genotypes were propagated so that there would be a good quantity and quality of virus to work with when conducting test runs. When propagating viruses, plaque assays were conducted to visualize how well the virus would plaque. Viruses that did plaque well were measured at random to estimate the expected ranges for the titer of the other valves. Titers helped determine how much virus one valve holds after collecting and flash-freezing at -80 degrees. I was trained on plaque reduction neutralization assays; this assay measures how many antibodies can be neutralized by the virus. PRN assays were used in response to the input of the virus for the number of viral plaques expected to show based on the respectable titer. I accomplished the proper training and experience working with federal agencies as a professional scientist.

• Assisted department with special projects and tasks to meet deadlines.
• Assisted in day-to-day maintenance and responsibilities to facilitate operations of assigned department.
• Produced comprehensive research questions and potential hypotheses.
• Evaluated potential risks associated with conducting experiments involving hazardous materials.
• Utilized specialized equipment such as microscopes, spectrometers, chromatographs and centrifuges to conduct experiments.
• Designed protocols for new experiments and implemented them in the lab setting.
• Conducted investigations into outbreaks of food-borne illness or infectious disease.

During my academic years, I conducted research on human cytomegalovirus (HCMV) infection at the maternal-fetal interface, focusing on signaling responses, various innate immune pathways, and the immune system's reaction to HCMV. I utilized several assays, including sandwich ELISA, which was particularly suited to our research goals due to its specificity and reliability. I was proficient with Western blot techniques to analyze protein activity within these pathways when exposed to infection. I quickly mastered the technique with the support of my team, and soon performed the procedures independently, with precision and confidence. I had prior experience with qPCR; however, it presented challenges that required extensive troubleshooting. This process involved recalculating my primer concentrations, adjusting the amount of cDNA, verifying concentrations using a NanoDrop, and ensuring proper mixing by centrifuging the plate.

This research experience not only deepened my understanding of viral infection mechanisms, but also allowed me to enhance my technical skills, including troubleshooting complex processes, and employing a range of laboratory techniques with confidence. Here, I accomplished gaining knowledge of a common clinical infection for pregnant women, and advanced strategies for scientific processes.

• Learning how to troubleshoot effectively, based on experimental needs.
• Practice and promote health equity among communities.
• Take on the role of a leader by helping to assist other students with their project.

As an assistant researcher under a master's student and academic advisor, I contributed to a project focused on the characteristics of the PAH1 phosphatase and its differentiated effects on gene expression and lipid biosynthesis. My responsibilities included preparing YPD broth, isolating and purifying DNA, and analyzing protein curves. I also received training on various laboratory instruments, such as the autoclave, mega and mini centrifuges, and techniques for accurately preparing broth and analyzing protein data.

This opportunity significantly enhanced my skill set in biotechnology, allowing me to grow both scientifically and technically. My contributions helped generate valuable results for the master's student's defense, and the successful acceptance of the article for publication. I accomplished the ability to bring more opportunities for students who were interested in expanding their scientific skills.

• Holding seminars to discuss my role as an assistant researcher.
• Generating plans with my team to secure more funding for student opportunities.
• Introducing ideas to the department to bring light to student work, but in specialized laboratories.

During my internship, I conducted research on the effects of dietary supplements on Type 2 diabetes, using Zucker fatty rat models. The study involved euthanizing the animals, and homogenizing their tissues for further analysis. The processed tissue samples were then tested using a Qubit fluorometer to quantify DNA, RNA, and protein levels. The Qubit was particularly effective in distinguishing between intact and degraded RNA, ensuring the reliability and accuracy of the results.

I utilized relevant literature to guide the selection of appropriate methodologies, which contributed to improving the efficiency of the project. My efforts led to the achievement of exceptional results, with significant findings related to proactive prevention factors for Type 2 diabetes. These were supported by both preliminary data and the final outcomes of the project. This experience not only expanded my technical skills but also enhanced my ability to analyze complex data in a research setting. Accomplished project efficiency by more than 10%:

• Keeping team communication open about deadlines and objectives.
• Conduct routine maintenance (monthly).
• Evaluation of lab processes.