Summary
Overview
Work History
Education
Skills
Websites
Affiliations
Awards
Publications
References
Hobbies and Interests
Timeline
Generic

RAFAEL E. FLORES-OBANDO

Brooklyn,NY

Summary

Talented Postdoctoral Associate with over six years of research experience eager to advance field knowledge of lipid-related mechanisms of pulmonary diseases. Resourceful and adaptable individual with expertise in implementing isolation, culture, and genetic manipulation of primary rodent brain cells. Strong track record in designing, executing, and publishing intricate research projects, utilizing a diverse range of cellular and molecular biology tools, including confocal microscopy.

Overview

18
18
years of professional experience

Work History

Postdoctoral Associate

State University of New York, Downstate Medical Center
07.2019 - Current
  • Contributed to and actively participated in research conception, design, and execution to assess interaction of two putative brain proteins (PKMζ and KIBRA) involved in memory using fluorescence-based assays such as Bimolecular Fluorescence Complementation (BiFC) leading to 1 publication in Science Advances.
  • Conducted immunofluorescence staining and confocal microscopy imaging on brain sections to detect changes in the amount and distribution of PKMζ in brain sections leading to 1 publication in the European Journal of Neuroscience.
  • Performed isolation, culture, and genetic manipulation of primary hippocampal neurons to study PKMζ dynamics using live cell imaging with photoswitchable fluorescent proteins such as Dendra2.

Postdoctoral Associate

State University of New York, Downstate Medical Center
01.2018 - 06.2019
  • Implemented isolation and culture of primary oligodendrocytes, microglia, and astrocytes from rodents to examine their interaction in cell culture model of ischemic stroke as well as mechanisms that link cerebrovascular disease and Alzheimer's Disease.

Graduate Research Assistant

State University of New York, Downstate Medical Center
01.2011 - 01.2017
  • Designed and conducted experiments in a mouse model of leukodystrophy to study mutations in Connexin47 (Cx47) in primary mouse oligodendrocytes leading to 1 publication in Molecular and Cellular Neuroscience.
  • Developed protocols to isolate, culture, and genetically manipulate primary mouse oligodendrocytes leading to 1 publication in the Journal of Visualized Experiments.
  • Collaborated in 2 projects that studied heteromeric channel formation by mutant Connexin47 using cell culture, transfection, and immunofluorescence leading to 2 publications in Glia and the Journal of Neurology.
  • Mentored undergraduate and middle-school students, providing guidance on research methodologies and techniques.

Research Assistant

State University of New York, Downstate Medical Center
01.2009 - 01.2011
  • Performed laboratory maintenance (instruments check-up, ordering of reagents and materials).
  • Performed genotyping of DNA polymorphisms in clinical samples for ongoing case-control studies and SNP prioritization for Whole Genome Association Study leading 8 publications in journals like Carcinogenesis and Prostate.
  • Prepared IRB research protocols, designed research proposals, and meta-analyses, and trained graduate and undergraduate students.
  • Reviewed technical and professional publications and journals to stay current on recent literature and make more strategic research decisions.

Graduate Student Researcher

University of Pittsburgh, Graduate School of Public Health
01.2006 - 01.2009
  • Performed Fluorescence in situ hybridization, qRT-PCR, western blot, tissue culture, flow cytometry, clonogenic cell survival analysis, cell proliferation assays, siRNA, and foci formation procedures to investigate Fanconi Anemia pathway in head and neck cancer cell lines as well as small molecule inhibitors as therapeutic agents.

Education

PhD in Molecular and Cellular Biology - Dept. of Neurology and Pathology

State University of New York, Downstate Medical Center
Brooklyn, NY
12.2017

Master of Science in Human Genetics - Department of Human Genetics

University of Pittsburgh, Graduate School of Public Health
Pittsburgh, PA
06.2009

Bachelor of Science in Clinical Bioanalysis -

Universidad Nacional Autόnoma De Nicaragua
Managua, Nicaragua
01.1996

Skills

  • Cell culture
  • Immunofluorescence
  • Confocal microscopy
  • Working with rodents
  • Tissue processing
  • Western Blot
  • qPCR
  • Data Collection and Analysis
  • GraphPad Prism
  • Lab Safety
  • Project Management
  • Experimental design

Affiliations

  • New York Academy of Sciences (NYAS)
  • Society for Advancement of Chicanos and Native Americans in Science (SACNAS)
  • Society for Neuroscience (SfN)

Awards

  • Trainee Professional Development Award (TPDA) / Society for Neuroscience, August 2023.
  • Annual Research Day Poster Full Travel Award. SUNY Downstate Medical Center. Title: The role of mutations in Cx47 in myelinating cells of the central nervous system, May 2017.
  • Dean's Day Award at the 33rd Annual Honors Convocation of the University of Pittsburgh for the research project: Smoking, Chromosome Damage and an Abnormal DNA Damage Response (DDR) in Head and Neck Cancer (SCCHN), 2009.
  • Fulbright Scholarship Award, Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pennsylvania, 2006-2008.
  • BARSA Scholarship Award. Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pennsylvania, 2006-2008.
  • Hugo Arechiga Fellowship. Ricardo Miledi Neuroscience Training Program. Neurobiology Institute, Universidad Nacional Autonoma de Mexico (UNAM), Campus Queretaro, Mexico, Summer 2005.

Publications

  • KIBRA anchoring the action of PKMζ maintains the persistence of memory, Tsokas P†, Hsieh C†, Flores-Obando RE†, Bernabo M, Tcherepanov A, Hernández AI, Thomas C, Bergold PJ, Cottrell JE, Kremerskothen5, Shouval HZ, Nader K, Fenton AA, Sacktor TC, Science Advances, In Press, 2024-06 († Co-first authors).
  • Activation of the unfolded protein response by Connexin47 mutations associated with Pelizaeus-Merzbacher-like disease, Flores-Obando RE, Freidin MM, Hernández AI, Abrams CK, Mol Cell Neurosci, 120, 103716, 2022-05, 10.1016/j.mcn.2022 103716.
  • Persistent increases of PKMζ in memory-activated neurons trace LTP maintenance during spatial long-term memory storage, Hsieh C, Tsokas P, Grau-Perales A, Lesburguères E, Bukai J, Khanna K, Chorny J, Chung A, Jou C, Burghardt NS, Denny CA, Flores-Obando RE, Hartley BR, Rodríguez Valencia LM, Hernández AI, Bergold PJ, Cottrell JE, Alarcon JM, Fenton AA, Sacktor TC, Eur J Neurosci, 54, 8, 6795-6814, 2021-10, 10.1111/ejn 15137.
  • Investigating oligodendrocyte connexins: Heteromeric interactions between Cx32 and mutant or wild-type forms of Cx47 do not contribute to or modulate gap junction function, Abrams CK, Flores-Obando RE, Dungan GD, Cherepanova E, Freidin MM, Glia, 69, 8, 1882-1896, 2021-08, 10.1002/glia.23999.
  • Interactive effect of TLR SNPs and exposure to sexually transmitted infections on prostate cancer risk in Jamaican men, Dubey B, Jackson M, Zeigler-Johnson C, Devarajan K, Flores-Obando RE, McFarlane-Anderson N, Tulloch-Reid M, Aiken W, Kimbro K, Reed D, Kidd LR, Gibbs D, Kumar S, Ragin C, Prostate, 80, 15, 1365-1372, 2020-11, 10.1002/pros.24067.
  • Proceedings from the Albert Charitable Trust Inaugural Workshop on white matter and cognition in aging, Sorond FA, Whitehead S, Arai K, Arnold D, Carmichael ST, De Carli C, Duering M, Fornage M, Flores-Obando RE, Graff-Radford J, Hamel E, Hess DC, Ihara M, Jensen MK, Markus HS, Montagne A, Rosenberg G, Shih AY, Smith EE, Thiel A, Tse KH, Wilcock D, Barone F, Geroscience, 42, 1, 81-96, 2020-02, 10.1007/s11357-019-00141-8.
  • Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes, Flores-Obando RE, Freidin MM, Abrams CK, J Vis Exp, 135, 10.3791/57543, 2018-05-21.
  • Interactive effect of TLR SNPs and exposure to sexually transmitted infections on prostate cancer risk in Jamaican men, Dubey B, Jackson MD, Zeigler-Johnson C, Devarajan K, Flores-Obando RE, McFarlane-Anderson N, Tulloch-Reid MK, Aiken W, Kimbro K, Jones DZ, Kidd LR, Ragin C, Gene, 636, 96-102, 2017-12-15, 10.1016/j.gene.2017.09.034.
  • A new mutation in GJC2 associated with subclinical leukodystrophy, Abrams CK, Flores-Obando RE, Dungan GD, Cherepanova E, Freidin MM, J Neurol, 261, 10, 1929-1938, 2014-10, 10.1007/s00415-014-7432-7.
  • Melatonin and the Charlson Comorbidity Index (CCI): the Treviso Longeva (Trelong) study, Gallucci M, Flores-Obando R, Mazzuco S, Ongaro F, Di Giorgi E, Boldrini P, Durante E, Frigato A, Albani D, Forloni G, Zanardo A, Siculi M, Caberlotto L, Taioli E, Int J Biol Markers, 2014-02-13.
  • HPV-associated lung cancers: an international pooled analysis, Ragin C, Obikoya-Malomo M, Kim S, Chen Z, Flores-Obando R, Gibbs D, Koriyama C, Aguayo F, Koshiol J, Caporaso NE, Carpagnano GE, Ciotti M, Dosaka-Akita H, Fukayama M, Goto A, Spandidos DA, Gorgoulis V, Heideman DA, van Boerdonk RA, Hiroshima K, Iwakawa R, Kastrinakis NG, Kinoshita I, Akiba S, Landi MT, Eugene Liu H, Wang JL, Mehra R, Khuri FR, Lim WT, Owonikoko TK, Ramalingam S, Sarchianaki E, Syrjanen K, Tsao MS, Sykes J, Hee SW, Yokota J, Zaravinos A, Taioli E, Carcinogenesis, 2014-02-26.
  • The impact of genetic variants in inflammatory-related genes on prostate cancer risk among men of African Descent: a case control study, Jones DZ, Ragin C, Kidd NC, Flores-Obando RE, Jackson M, McFarlane-Anderson N, Tulloch-Reid M, Kimbro KS, Kidd LR, Hered Cancer Clin Pract, 11, 1, 19, 2013-12-23, 10.1186/1897-4287-11-19.
  • Polymorphisms in CYP17 and CYP3A4 and prostate cancer in men of African descent, Taioli E, Sears V, Watson A, Flores-Obando RE, Jackson M, Ukoli FA, de Syllos Cólus IM, Fernandez P, McFarlane-Anderson N, Ostrander EA, Rodrigues IS, Stanford JL, Taylor JA, Tulloch-Reid M, Ragin CC, Prostate, 73, 6, 668-676, 2013-05, 10.1002/pros.22619.
  • Multi-institutional prostate cancer study of genetic susceptibility in populations of African descent, Taioli E, Flores-Obando RE, Agalliu I, Blanchet P, Bunker CH, Ferrell RE, Jackson M, Kidd LC, Kolb S, Lavender NA, McFarlane-Anderson N, Morrison SS, Multigner L, Ostrande EA, Park JY, Patrick AL, Rebbeck TR, Romana M, Stanford JL, Ukoli F, Vancleave TT, Zeigler-Johnson CM, Mutetwa B, Ragin C, Carcinogenesis, 32, 9, 1361-1365, 2011-09, 10.1093/carcin/bgr135.
  • Polymorphisms in DNA damage response genes and head and neck cancer risk, Flores-Obando RE, Gollin SM, Ragin CC, Biomarkers, 15, 5, 379-399, 2010-08, 10.3109/1354750X.2010.494051.
  • The human promyelocytic leukemia protein is a tumor suppressor for murine skin carcinogenesis, Virador VM, Flores-Obando RE, Berry A, Patel R, Zakhari J, Lo YC, Strain K, Anders J, Cataisson C, Hansen LA, Yuspa SH, Mol Carcinog, 48, 7, 599-609, 2009-07, 10.1002/mc.20507.
  • Genomic and proteomic approaches for Chagas' disease: critical analysis of diagnostic methods, Huete-Pérez JA, Flores-Obando RE, Ghedin E, Caffrey CR, Expert Rev Mol Diagn, 5, 4, 521-530, 2005-07, 10.1586/14737159.5.4.521.
  • Allele frequencies for markers CSF1PO, TPOX, TH01, F13A01, FESFPS, vWA, D16S539, D7S820, D13S317 in the general population of Nicaragua, Flores-Obando RE, Budowle B, Huete-Pérez JA, J Forensic Sci, 49, 2, 416-417, 2004-03.

References

  • Todd C. Sacktor, MD, Distinguished Professor Physiology and Pharmacology Neurology, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY, 11203, (718) 270-3933, todd.sacktor@downstate.edu
  • Alejandro Ivan Hernandez, Ph.D., Assistant Professor of Pathology, SUNY Downstate Medical Center, 450 Clarkson Ave, Rm 4-109, Box 25, Brooklyn, NY, 11203, (718) 270-4561, ivan.hernandez@downstate.edu
  • Frank C. Barone, Ph.D., Professor of Neurology, Professor of Physiology & Pharmacology Director of Basic Research, Cerebrovascular Division, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY, 11203, (718) 221-5745, frank.barone@downstate.edu
  • Charles K. Abrams MD, Ph.D., Professor, Department of Neurology and Rehabilitation, University of Illinois at Chicago, (312) 355-5859, cabrams1@uic.edu

Hobbies and Interests

  • Photography
  • Running
  • Guitar

Timeline

Postdoctoral Associate

State University of New York, Downstate Medical Center
07.2019 - Current

Postdoctoral Associate

State University of New York, Downstate Medical Center
01.2018 - 06.2019

Graduate Research Assistant

State University of New York, Downstate Medical Center
01.2011 - 01.2017

Research Assistant

State University of New York, Downstate Medical Center
01.2009 - 01.2011

Graduate Student Researcher

University of Pittsburgh, Graduate School of Public Health
01.2006 - 01.2009

PhD in Molecular and Cellular Biology - Dept. of Neurology and Pathology

State University of New York, Downstate Medical Center

Master of Science in Human Genetics - Department of Human Genetics

University of Pittsburgh, Graduate School of Public Health

Bachelor of Science in Clinical Bioanalysis -

Universidad Nacional Autόnoma De Nicaragua
RAFAEL E. FLORES-OBANDO