Summary
Overview
Work History
Education
Skills
Certification
Accomplishments
Timeline
Generic

Rohini Bhattacharya

Union City,CA

Summary

Results-driven professional with 9 years of experience in Molecular Biology, Immuno-oncology, and Bioinformatics research. Skilled in both academic and industry settings, with exceptional decision-making abilities and a talent for building client relationships. Proficient in multitasking, time management, and conflict resolution. Demonstrates leadership through leading by example and fostering collaboration, resulting in positive team dynamics and the professional growth of junior scientists. Passionate about mentoring and guiding team members towards success, continuously striving for innovation and excellence in all projects.

Overview

10
10
years of professional experience
1
1
Certification

Work History

Bio Assay Scientist

Eurofins Scientific
03.2022 - Current
  • Mammalian cell culture with a variety of cancer cell types.
  • Development of cell-based assays for analyzing drug activity.
  • Design and execution of relative potency assays.
  • Analysis of pharmacology data from cell-based assays using GraphPad Prism or similar software.
  • Analysis of data from relative potency assays using PLA 3.0, SoftMax Pro or similar software package.
  • Running cell-based cytotoxicity assays using CAR-T or other primary cells such as PBMCs or T Cells.
  • Optimization of culturing and freezing conditions for large scale manufacture of assay-ready cells.
  • Data entry into relational databases, such as FileMaker, for capture of manufacturing and experimental details.
  • Ability to write detailed SOPs to document new assays/methods.

Senior Associate Scientist

Mersana Therapeutics
09.2021 - 03.2022
  • Lead, design, and execute in vitro studies to characterize antibody-drug conjugate (ADC) bystander activity and support identification and validation of novel ADC targets.
  • Advance Dolasynthen and Immunosynthen ADC projects from antibody discovery through IND submission.
  • Performed specialized experiments and coordinated with external CROs to support revised treatment strategies.
  • Derive treatment-resistant cell lines and explore combination strategies to support unmet patient need.
  • Spearhead Research Journal Club where inter-departmental discourse on antibody and ADC innovations inform data interpretation and pipeline considerations.
  • Led cross-functional teams to achieve project milestones on time and within budget constraints.

Senior Research Technician (Dr. Luis Diaz lab)

Memorial Sloan Kettering Cancer Center
12.2019 - 09.2021
  • Created and implemented new research methods to explore mechanisms of biological processes.
  • Applied good documentation practices when recording data and managing source documentation.
  • Authored and co-authored publications in peer-reviewed journals, showcasing the validity and importance of conducted research.
  • Leveraged statistical analysis tools to interpret complex data sets, providing key insights for decisionmakers.
  • Presented research findings at conferences, increasing visibility of the organization''s work within the scientific community.

Research Analyst II

Charles River Laboratories
08.2016 - 09.2019
  • Performed laboratory analyses, including Ames assay, chromosomal aberration assay, HPBL, and TK6 screening assays and in vitro micronucleus assays under supervision of the Study Director / Principal Investigator.
  • Optimized mammalian cell splitting and cleansing schedule and provided reference guides, ensuring sufficient cultures were available for weekly assays.
  • Gained insights into drug development and safety assessment through continuous education courses.
  • Record project data in accordance with GLP regulations.
  • Generate recombinant cell lines including preparing expression constructs, performing transfection, pool selection, clone screening, selection and cell banking.

Graduate Research Assistant

DePaul University
09.2014 - 07.2016
  • Immunology Research
  • Mutation of SH3 binding site in chicken B cells
  • Experience in tissue culture, changing media, feeding cells
  • Single cell cloning
  • Experience developing and using parallel purification system for mAbs
  • Expert in purification from mammalian cell supernatant, cell lysates and inclusion bodies
  • Molecular biology and protein expression
  • Molecular cloning and expression vector construction
  • Transfection of prokaryotic cells into eukaryotic cells
  • Expression in inclusion bodies and protein refolding
  • Western blotting, ELIZA
  • Immunofluorescence using primary and secondary antibodies
  • Other analytical techniques

Education

Master's degree - Biology, Immunology

DePaul University
01.2016

Bachelor's degree - Biotechnology

Manipal Institute of Technology
01.2012

Skills

  • Cell Line Development
  • Immune cell isolation
  • multi-color flow cytometry
  • IncuCyte
  • Octet
  • Luminex
  • Genome sequencing
  • library prep
  • PCR, DNA & protein isolation & quantification
  • ELISA, cell culture
  • FISH
  • Transfection
  • , Western blot
  • Micronucleus (HPBL, CHO, TK6, in vivo)
  • Chromosome Aberration
  • Neutral Red Uptake
  • Mouse Lymphoma
  • CHO/HPRT
  • Comet
  • High-throughput screens

Certification

  • Project management
  • Analyzing and Visualizing Data

Accomplishments

    PUBLICATIONS, ABSTRACTS, POSTERS & PRESENTATIONS.

  • R.Bhattacharya, C.ovies, D.Williamson, S.Mitchell and P.E.Funk, Sh3 Dependent cell death signaling of the Avian chB6 Allonantigen, Cell. Immunol.
  • Wells M.; Lorenz M.; Somer-James J.; Kaye S.L.; Gromer K.D.; Bhattacharya R.; Oco K.R.; KWOK V.Y.; Farabaugh C.S.; Stankowski L.F. Jr, 56 th Annual SOT meeting and Toc Expo Validation and comparison of the In Vitro Micronucleus Assay in TK6 and CHO-WBL cells ( Abtr), 2017.
  • Funk, P.E, Bhattahcharya, R. Mutagenic Analysis of SH3 binding within chB6 alloantigen. J. Immunol. 194: 61.1 (Abstr.), 2016
  • Bhattacharya.R, Funk,P.E, 43rd autumn auto immunology conference “Mutagenic Analysis of SH3 binding within chB6 alloantigen”(Abstr) 2015.
  • Simultaneous assessment of somatic mutations from multiple biofluids (Manuscript in progress)
  • Induction of antitumor immunity by tailored chemical mutagenesis (Manuscript in progress)

Timeline

Bio Assay Scientist

Eurofins Scientific
03.2022 - Current

Senior Associate Scientist

Mersana Therapeutics
09.2021 - 03.2022

Senior Research Technician (Dr. Luis Diaz lab)

Memorial Sloan Kettering Cancer Center
12.2019 - 09.2021

Research Analyst II

Charles River Laboratories
08.2016 - 09.2019

Graduate Research Assistant

DePaul University
09.2014 - 07.2016

Bachelor's degree - Biotechnology

Manipal Institute of Technology

Master's degree - Biology, Immunology

DePaul University

Similar Profiles

CREATE PROFILE
Rohini Bhattacharya