Work History
Education
Skills
References
Timeline
Generic

Ryan Day

Rockville,MD

Work History

Student Assistant for Montgomery College Biotech department

07.2024 - 08.2024

Student Assistant for the NISTCHO project

09.2024 - 05.2024
  • Managed two student projects (Banking/Thawing and Bioreactors), Presented research done on NISTCHO project at the Maryland Collegiate Stem Conference

Education

AAS Biotechnology -

Montgomery College
05.2024

Skills

  • Cell Culture Aseptic technique (CHO cell culture, suspension and adherent, NISTCHO, CHO K1), media and buffer preparation
  • Cell banking (thawing vials, expanding cell lines, freezing cells in DMSO)
  • Microbiology aseptic technique and basic bacterial staining (gram staining, endospore staining, acid fast staining), Microscope use, compound light and dissecting
  • Bioreactor; batch and fed batch (growing seed stock, setup of vessel, seeding, monitoring, sampling, feeding, harvesting)
  • FPLC (buffer prep, sample prep, affinity and ion exchange chromatography), Tangential Flow Filtration, Ultrafiltration, Monoclonal Antibody purification
  • Student Project management
  • Total RNA extraction, cDNA synthesis, PCR and RT qPCR, Nanodrop use, Restriction Enzyme digest and DNA cloning, agarose gel electrophoresis
  • Tissue homogenization, BCA and Coomassie blue assays, SDS PAGE, ELISA and Western Blot

References

References available upon request

Timeline

Student Assistant for the NISTCHO project

09.2024 - 05.2024

Student Assistant for Montgomery College Biotech department

07.2024 - 08.2024

AAS Biotechnology -

Montgomery College
Ryan Day