Sweta Khanal
2501 West 7th St. #106, Hattiesburg,MS
Summary
- Enthusiastic, organized and efficient professional with 7+ years of hands-on experience in research.
- Specialized in molecular biology, small RNA biology, RNAi and NGS data analysis.
- Self-motivated, able to work independently and within team, strong knowledge, planning and problem solving skills, trained and mentored undergraduate students, able to establish and maintain good working relations with multi-disciplinary/cultural national/international people
Overview
8
8
years of professional experience
Work History
Graduate Research Assistant
University Of Southern Mississippi
08.2017 - Current
- Investigated role of non-canonical microRNA, mir-1017, in Drosophila melanogaster utilizing CRISPR/Cas9 system
- Studied small RNA population in marine polychaete
- Gained experience in small RNA isolation, bioanalyzer and NGS data analysis
- Investigating processing of mammalian mirtron mir-5010 in HEK cells using siRNA mediated knockdown and northern blot
- Exploring function of somatic piRNAs in Capitella teleta
- Experiment design, plan, trouble shooting and data analysis
- Trained summer interns, undergraduate student and honors student and in the lab
- Mississippi INBRE Research Scholars instructor (2021-2022)
Teaching Assistant
University Of Southern Mississippi
08.2021 - 05.2022
- Lab instructor for BIO 110L and Histology 461/561
- Prepared lessons according to course outline to convey required material and deepen student understanding of subject matter
- Partnered with teacher to plan and implement lessons following school's curriculum, goals and objectives.
- Checked assignments, proctored tests, and provided grades according to university standards.
- Streamed classes online and uploaded videos for later viewing by distance and absent students.
Lecturer, Biology
Universal Science College, Kathmandu, Nepal
01.2014 - 12.2015
- Take course on clinical biochemistry and bioinformatics
- Conduct and guide on dry and wet lab for undergraduate students
- Designed and distributed lecture handouts to round out material and increase student understanding.
Lab Instructor
Lord Buddha Education Foundation, Kathmandu, Nepal
08.2013 - 11.2013
- Conduct and guide laboratory works for undergraduate and graduate students
- Discuss the protocols, results and interpretation of laboratory activities
- Observed students to assess progress with laboratory work and assign grades.
- Demonstrated skills and safety procedures to students during laboratory sessions.
- Evaluated and supervised student activities and performance levels to provide reports on academic progress.
Education
Ph.D. - Cellular And Molecular Biology
University of Southern Mississippi
Hattiesburg, MS06.2023
Master of Science - Biotechnology
South Asian University
New Delhi, India05.2013
Skills
- DNA isolation, PCR, Agarose electrophoresis, cloning
- RNA isolation, qPCR, In vitro transcription, Northern blot
- Mammalian cell culture, stable cell line development, transfection, western blot, Immunoprecipitation
- Next Generation Sequencing data analysis
- CRISPR, Microinjection
Publications
- Khanal S, Zancanela BS, Peter JO, Flynt AS. The Small RNA Universe of Capitella teleta. Front Mol Biosci. 2022 Feb 25;9:802814. doi: 10.3389/fmolb.2022.802814. PMID: 35281272; PMCID: PMC8915122.
- Kumar S, Gupta P, Khanal S, Shahi A, Kumar P, Sarin SK, Venugopal Sk. Over-expression of microRNA-30a inhibits HBX-induced autophagosome formation in hepatic cells. FEBS (DOI: 10.1111/febs.13209)
Scientific Presentations
- Poster presentation on “Positional Significance of Branch Point In Maturation of Tailed Mirtrons” on Mississippi Academy of Sciences, 2018, March 31, Hattiesburg, Mississippi, U.S.A
- Poster presentation on “Sequence elements in the tail of 3’ tailed mirtron is critical for its biogenesis” on Mississippi Academy of Sciences, 2019, The University of Southern Mississippi, Hattiesburg, Mississippi, U.S.A
- Oral presentation on “Modulation of Acetylcholine receptor activity by a non-canonical microRNA” on IDeA Conference, 2019, Hilton Jackson, Mississippi, U.S.A
- Virtual Poster Presentation on “small RNA Universe of Capitella teleta” on SEiDeA 2021, Nov 14, Puerto Rico, USA
- Oral presentation on “small RNA Universe of Capitella teleta” on Mississippi Academy of Sciences, 2022, March 31, Gulfcoast Convention centre, Biloxi, Mississippi, U.S.A
- Poster Presentation “small RNA Universe of Capitella teleta” Experimental biology 2022, Apr 2-5, Philadelphia, PA, USA
- Oral presentation on “ Regulation of Acetylcholine receptors by a non-canonical miRNA in Drosophila melanogaster ” on IDeA Conference, July 28, 2022, Hattiesburg, Mississippi, U.S.A
Awards and Scholarship
- South Asian University Scholarship; 2011-2013
- Graduate school travel award, USM, Apr 2022
- BEES Travel award, USM, Spring 2022
Timeline
Teaching Assistant
University Of Southern Mississippi
08.2021 - 05.2022
Graduate Research Assistant
University Of Southern Mississippi
08.2017 - Current
Lecturer, Biology
Universal Science College, Kathmandu, Nepal
01.2014 - 12.2015
Lab Instructor
Lord Buddha Education Foundation, Kathmandu, Nepal
08.2013 - 11.2013
Ph.D. - Cellular And Molecular Biology
University of Southern Mississippi
Master of Science - Biotechnology
South Asian University
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