Summary
Overview
Work History
Education
Skills
Websites
Abstracts Presented
Publications
Instruments Of Expertise
Research
Timeline
Generic

Vaishnavi Beena Valsan

San Francisco,USA

Summary

Cell biologist with several years of experience in cellular research seeking to contribute expertise in cell-based assay development, cell binding assays, cell line development. Analytical chemist and immunologist with comprehensive knowledge in proteomics (experimental design, sample preparation, mass spectrometry, and data analysis). Performed several cell-based assay development techniques at pre-clinical team and drug discovery team, experiments include gene expression, Western blot, ELISA. Familiar with a variety of research techniques integral to genomics, molecular biology, immunology and biochemistry; adept at applying critical thinking about current literature to troubleshoot research difficulties; talented at learning new techniques; thrives when multitasking between multiple projects and meet deadlines under pressure with positive attitude; self- motivated and able to work independently or in a team; good at giving presentations.

Overview

4
4
years of professional experience

Work History

Senior Research Associate I

Lycia Therapeutics
South San Francisco, CA
11.2023 - Current
  • Maintained a variety of mammalian cell lines (Hep G2, SNU899, Human primary hepatocytes, NS-1, 293T, different kinds of KO and clonal cell lines
  • Performed live cell imaging for various kinds of mammalian cell lines to understand the uptake, lysosomal degradation of different LYTAC compounds by using different fluorescence reagents via Incucyte live cell imaging machine
  • Performed in-vitro cell-based assays via flow cytometry to understand the cellular uptake of different LYTAC compounds in different mammalian cells
  • Performed in-vitro cell surface staining of NS-1 clones with fluorescent dyes and antibodies to characterize the knock-out NS-1 clones
  • Served as ex-vivo lead for PK/PD and efficacy studies to achieve crucial milestones for different projects by performing different types of ELISA
  • Supported in-vitro studies by optimizing flow cytometry, live cell imaging, ELISA and MSD assays in various kinds of mammalian cell lines
  • Designed and executed the study, generated data and slide decks presented to the project team
  • Performed MSD assays to test different LYTAC compounds
  • Maintained ELN by writing experiment objectives, methods, results and detailed protocol and submitted the data to the company’s database
  • Contributed to other project team by performing different in-vitro studies
  • Performed screening of cell lines by performing HiBiT assays in different Knock-in/Knock out cells to observe the receptor expression and uptake
  • Maintained the cell line inventory by expanding different cell lines and generating cell banks, updated the database for the biology team in the company
  • Aliquoted FBS, Pen/Strep, managed to check the cell line inventory sheet when needed
  • Coordinate activities across functional groups and project teams
  • Analyze, document, and report data concisely and rigorously
  • Communicate within interdisciplinary team by presenting experimental results, analysis, and interpretations at internal meetings

Research Associate

Kezar Life Sciences
South San Francisco, CA
01.2023 - 10.2023
  • Optimized and performed sample preparation of LC-MS proteomics samples utilizing secretome and subcellular fractionation-based methods
  • Utilized Proteome Discoverer 3.0 and ChimerysTM search engine for analysis of mass spectrometry data
  • Maintained a variety of cancer and immunological cell lines (H929, Jurkat, U266, CAL27, SK-BR-3, BxPC-3, MiaPaCa2, SNU899, T cells)
  • Performed in-vitro time-dependent and dose-dependent dosing and transfection of cell lines
  • Prepared and processed samples for target validation via the JESSTM system (western blot) and QuantStudio 6 (qRT- PCR)
  • Updated experiments protocols/documentation on ELN
  • Communicate within interdisciplinary team by presenting experimental results, analysis, and interpretations at internal meetings

Research Associate II

Mantra Bio
South San Francisco, CA
09.2021 - 01.2023
  • Maintained a variety of human and mouse cell lines (HUVEC, MRMEC, NIH3T3, MKGEC, HRMEC, RAW264.7)
  • Performed in-vitro time-dependent and dose-dependent dosing and transfection of cell lines
  • Performed in vitro cell based assay development to obtain pVEGF/VEGF, pSTAT/STAT3 signals through ELISA
  • Performed in-vitro studies utilizing the JESSTM (western blot) and QuantStudio 6 (qRT-PCR) systems
  • Performed LPS induced RAW 264.7 cells time point dosing, collection, and gene expression studies by various genes
  • Conducted media adaptation studies for different kinds of cell lines
  • Updated the cell bank and media lot information in database through POSTICO and DATABASE API system
  • Assisted other departments by maintaining cells for different kinds of cell based and vFC flow cytometric analysis
  • Updated experiments protocols/documentation on confluence page (JIRA CARDS)
  • Communicate within interdisciplinary team by presenting experimental results, analysis, and interpretations at internal meetings

Graduate Instrument Specialist for BD FACS Melody Cell Sorter & FACS Calibur Flow Cytometry

University of Illinois in Chicago
Chicago, USA
04.2021 - 08.2021
  • Helped graduate students in performing flow and sorter-based experiments
  • Maintenance of sterility (Long term shutdown and Daily shutdown - ethanol wash), change of sheath fluid & waste tank caps, cleaning of sorter nozzles by using ethanol and distilled water for the machine safety
  • Weekly checkup of machine performance and software checking
  • Troubleshooting the machine software with the help of BD instructor
  • Reported to BD instructor for machine repairment when needed

COVID-19 saliva collection staff

University of Illinois in Chicago
Chicago, USA
04.2021 - 05.2021
  • Processed saliva samples for SARS-COVID19 testing
  • Collected saliva samples manually, scanned and transferred into sterile test tubes and send it to SHIELD lab for performing RT-qPCR
  • All tasks were performed under strict safety guidelines instructed by university (Biohazard bags, Gloves & Masks)

Education

M.S. - Engineering Management

Trine University
Arizona, USA
12-2026

M.S. - Medical Biotechnology

University of Illinois
Chicago, USA
08.2021

B.TECH. - Biotechnology

SRM University
Chennai, India
05.2019

Skills

  • Mammalian cell culture technique
  • Plasmid design/construction
  • Plasmid transfection
  • Cell based assay development
  • Cell binding assays
  • Cellular uptake assay development
  • RNA extraction
  • RT
  • CDNA conversion
  • Q-RT-PCR
  • LC-MS/MS
  • Proteomics
  • Excel
  • GraphPad Prism
  • Confocal microscopy
  • Live cell imaging
  • ELISA
  • Western Blot
  • JESS
  • Cytotoxicity essays
  • Recombinant protein expression
  • Flow cytometry
  • Cell sorting
  • Mass spectrometry sample preparation method development
  • Cytokine bead array
  • Immunostaining
  • Nanoparticle characterization techniques

Abstracts Presented

  • Exploring implant-derived wear particle mediated macrophage polarization: TLR-2 and TLR-4 co-activation, Research week 2021, University of Illinois at Rockford
  • Exploring implant-derived wear particle mediated macrophage polarization: TLR-2 and TLR-4 co-activation, Third Annual UIC Bioengineering Research Symposium, University of Illinois in Chicago

Publications

  • Akshad Balde, Poulomi Ghosh, Parthasarathi Aishwaraya, Valsan Vaishnavi, Rasool Abdul Nazeer, Utilization of diamondback puffer (Lagocephalus guentheri) biomass for the production of bioactive oligopeptides and their inflammation suppressing effects in vitro, 2024
  • Holly, R. W., Hu, Y. Z., Valsan, V. B., Kougentakis, C., Petronikolou, N., Chen, T., ... & Shurtleff, M. J., Monitoring lysosomal catabolism: a sensitive probe for assessing targeted lysosomal degradation of extracellular proteins, 2024

Instruments Of Expertise

  • BD FACS Melody Cell Sorter
  • FACS Calibur Flow Cytometry
  • Protein Simple JESS
  • GEN 5 Plate Reader
  • BioTek Plate Washer
  • Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer
  • Tecan Plate Reader
  • Fluorescence Microscope
  • Keyence Microscope
  • QPCR (Quantstudio 3, Quantstudio 6)
  • Thermo Scientific Nanodrop
  • Neon Transfection System
  • ExplorisTM 480- Vanquish Neo-FAIMS Pro Duo
  • Sony cell sorter
  • Incucyte live cell imaging machine

Research

  • Identification of Cellular Uptake Mechanisms Induced by CoCrMo Nanoparticle, 05/01/20, 08/31/21, Cellular uptake studies using cryo-TEM images and cytotoxicity assays.
  • Evaluating the reconstitution level of immune cells after adoptive transfer of lymphocytes, 01/01/21, 04/30/21, Tail snip was performed to draw blood samples from mouse every week.
  • Inhibitory effect of marine fish peptides and their expression on in vitro and in vivo models, 12/01/18, 05/31/19, The puffer fish muscle and visceral mass samples were hydrolyzed.
  • Antioxidant activity of encapsulated muscle protein hydrolysate from Red snapper fish, 12/01/17, 05/31/18, The objective of this study was to carry out encapsulation and characterization.

Timeline

Senior Research Associate I

Lycia Therapeutics
11.2023 - Current

Research Associate

Kezar Life Sciences
01.2023 - 10.2023

Research Associate II

Mantra Bio
09.2021 - 01.2023

Graduate Instrument Specialist for BD FACS Melody Cell Sorter & FACS Calibur Flow Cytometry

University of Illinois in Chicago
04.2021 - 08.2021

COVID-19 saliva collection staff

University of Illinois in Chicago
04.2021 - 05.2021

M.S. - Engineering Management

Trine University

M.S. - Medical Biotechnology

University of Illinois

B.TECH. - Biotechnology

SRM University
Vaishnavi Beena Valsan